Advances in propagation and conservation of medicinal plant species: A review


187

Authors

  • Tai Kharat Regional cum Facilitation Centre-Western Region (National Medicinal Plant Board, Ministry of Ayush, Government of India), Savitribai Phule Pune University, Pune , Maharashtra 411 007, India
  • D N Mokat Regional cum Facilitation Centre-Western Region (National Medicinal Plant Board, Ministry of Ayush, Government of India), Savitribai Phule Pune University, Pune , Maharashtra 411 007, India

https://doi.org/10.56093/ijas.v96i4.174617

Keywords:

Conservation, Dormancy, Medicinal Plants, Propagation, Tissue culture

Abstract

Many medicinal plants are on the verge of extinction from their natural habitats, due to global climate change, over-exploitation, anthropogenic activities, destructive harvesting, natural calamities, low quality seed set, low seed viability, pests and diseases etc. Hence there is an urgent need for medicinal plants propagation and conservation. Considering this, the current review is written on propagation methods of medicinal plants. The finding revealed that root and stem cutting treated with different plant growth regulators was the most effective practice for propagation of medicinal plants. This review examines the propagation of medicinal plants through sexual (seed), vegetative (stem and root cuttings) and in vitro micropropagation methods. Application of plant growth regulators has been shown to significantly improve propagation efficiency: IBA (1000 ppm) achieved 86–96% rooting and establishment, NAA and IAA (500–2000 ppm) yielded 70–90% rooting success and GA₃ (200–1000 ppm) enhanced seed germination to 85–97%.

             Promoting propagation and ex-situ conservation can help ensure sustainable supply, preserve genetic diversity and support commercialization of endangered medicinal plant species.

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References

1. Aadhan K and Anand S P. 2017. Documentation of rare endangered and threatened medicinal plants species in Sadhuragiri Hills, Tamil Nadu, India. International Journal of Botany Studies. 2 (1)32-37.

2. Ali M, Malik A R and Sharma K R. 2008. Vegetative propagation of Berberis aristata DC. An endangered Himalayan shrub. Journal of Medicinal Plants Research. 2(12) 374-377.

3. Amusa T O. 2011. Effects of three pre-treatment techniques on dormancy and germination of seeds of Afzelia africana (Sm. ex Pers). Journal of Horticulture and Forestry. 3(4) 96-103.

4. Aparna P M, Suryanarayana M A, Rajasekharan P E, Bhanuprakash K, Umesha K and Maruthi Prasad B N. 2018. Seed propagation studies in Embelia ribes Burm. F. Journal of Pharmacognosy and Phytochemistry. 3 380-383.

5. Arisdason W and Lakshminarasimhan P. 2016. Status of plant diversity in India: An overview. Central National Herbarium, Botanical Survey of India, Howrah.

6. Arumugam G, Sinniah U R, Swamy M K and Lynch P T. 2020. Micropropagation and essential oil characterization of Plectranthus amboinicus (Lour.) Sprengel, an aromatic medicinal plant. In Vitro Cellular & Developmental Biology – Planthttps://doi.org/10.1007/s11627-020-10056-1.

7. Arumugam KS and Thirumal S. 2011. Regeneration of In-vitro Plantlets in Hemidesmus indicus (L.) R. Br. through nodal and leaf explants. International Multidisciplinary Research Journal. 1(10)41-45.

8. Ayanoglu F, Mert A, Erdogan C and Kaya A. 2008. Propagation of some native grown medicinal plants by stem cuttings. Journal of Herbs, Spices & Medicinal Plants. 9 (4) 405-411.

9. Azhdari G, Tavili A and Zare M. 2010. Effects of various salts on the germination of two cultivars of Medicago sativa. Frontiers of Agriculture in China. 4 (1)63-68.

10. Bais H P, Sudha G, Suresh B and Ravishankar G A. 2000. Silver nitrate influences in vitro root formation in Decalepis hamiltonii Wight & amp; Arn. Current Science. 79(6) 894 -898.

11. Bapat V A, Yadav S R and Dixit G B. 2008. Rescue of endangered plants through biotechnological applications. National Academy Science Letters. 31 (7-8) 201-210.

12. Bhattacharya A K, Lahiri A K and Basu R N. 1991. Improvement of germination ability of Eucalyptus spp. by pre-germination treatments. Indian forester. 117662-663.

13. Biswas S, Rawat M S, Tantray F A and Sharma S. 2017. Medicinal plants conservation and development areas (MPCDAs)-An initiative towards conservation of medicinal plants. Medicinal plants. 9 (3) 143-149.

14. Chandrakar A K. 2014. Conservation of Medicinal plant Diversity in Gujarat. International Journal of Environment and Natural Sciences. 1 (2) 44-64.

15. Chauhan H K, Bisht A K, Bhatt I D and Bhatt A. 2020. Protocol for vegetative propagation of Trillium govanianum Wall ex D. Don. Journal of Applied Research on Medicinal and Aromatic Plants.16100233.

16. Chen B. 2009. In vitro propagation of a medicinal plant: Tripterygium wilfordii Hook f. Forestry studies in China. 11 (3)174–178.

17. Dakah A, Zaid S, Suleiman M, Abbas S and Wink M. 2014. In vitro propagation of the medicinal plant Ziziphora tenuior L. and evaluation of its antioxidant activity. Saudi Journal of Biological Sciences. 21 (4) 317–323.

18. Deepak K G K, Suneetha G and Surekha C H. 2016. A simple and effective method for vegetative propagation of an endangered medicinal plant Salacia oblonga Wall. Journal of Natural Medicines. 70(1) 115-9.

19. Denton O A, Oyekale K O, Nwangburuka C C, Daramola D S, Adeyeye J A Olukayode O O. 2013. Influence of high dry heat temperature on seed germination, seedling emergence and seedling vigour of three cultivars of Corchorus olitorius seeds. American Journal of Research Communication. 1(5) 98-114.

20. Deshpande R S, Neelakanta N T and Hegde N. 2006. Cultivation of crops and aromatic crops as a means of diversification in agriculture. Research Report: IX/ADRT/115.

21. Erica E B. 2000. In vitro Plant Recalcitrance: An Introduction. In Vitro Cellular & Developmental Biology Plant. 36 (3) 141-148.

22. Farnsworth N R and Soejarto D D. 1985. Potential consequences of plant extinction in the United States on the current and future availability of prescription drugs. Econ. Bot. 39(3) 231-240.

23. FRLHT. 2018. Medicinal plants of India- Guidelines for National policy of Environment and Forests, Govt. of India, New Delhi.

24. Ghosh S N and Sen S K. 1988. Effect of seed treatment on germination, seedling growth and longivity of Ber (Ziziphus mauritiana Lank) seeds. South Indian horticulture. 36 260-261.

25. Gopinath B, Gandhi K and Saravanan S. 2014. In vitro-propagation of an important medicinal plant Artemisia annua L. from axillary bud explants. Advances in Applied Science Research. 5(1) 254-258.

26. Goraya G S and Ved D K. 2017. Medicinal Plants in India: An Assessment of their Demand and Supply. National Medicinal Plants Board, Ministry of AYUSH, Government of India, New Delhi and Indian Council of Forestry Research & Education, Dehradun.

27. Govinden-Soulange J, Boodia N, Dussooa C, Gunowa R, Deensah S, Facknath S and Rajkomar B. 2009. Vegetative propagation and tissue culture regeneration of Hibiscus sabdariffa L. (Roselle). World Journal of Agricultural Sciences. 5 (5)651-661.

28. Gupta V. 2003. Seed germination and dormancy breaking techniques for indigenous medicinal and aromatic plants. Journal of Medicinal and Aromatic Plant Sciences. 23(2) 402- 407.

29. Hartmann H T and Kester D E. 1979. Plant Propagation Principles and Practices. Fourth Edition, Prentice Hall of India, Ltd., New Delhi.

30. Hartmann H T, Kester D E and Davies F T. 1990. Plant propagation Principles and practice. Prentice-Hall International: London 647 199-255.

31. Hartmann H T, Kester D E, Devies F T and Geneve R L. 2007. Plant propagation principles and practices. Seventh Edition, Prentice Hall of India Pvt. Ltd., New Delhi.

32. Isah T and Umar S. 2020. Influencing in vitro clonal propagation of Chonemorpha fragrans (moon) Alston by culture media strength, plant growth regulators, carbon source and photo periodic incubation. Journal of Forestry Research. 31(1) 27–43.

33. Kharat T D and Mokat D N. 2018. Floristic and Ecological studies of Mayureshwar and Rehekuri wildlife sanctuaries. International Journal of Botany Studies. 3(2) 29-37.

34. Kharat T D and Mokat D N. 2020. Histochemical and phytochemical study of drug Indian Sarsaparilla Hemidesmus indicus (L.) R. Br. ex Schult. International Journal of Botany Studies. 5(6) 344-350.

35. Kharat T D and Mokat D N. 2020. Pharmacognostic and phytochemical studies on Hemidesmus indicus and its substitute Decalepis hamiltonii – review. International Journal of Botany Studies. 5(4)224-231.

36. Kharat T D and Mokat D N. 2022. Comparative Study of Essential Oil Found in Roots of Wild and Cultivated Drug Anantmool. J. Essent. Oil-Bear. Plants, 24 (6):1227 – 1234.

37. Kharkwal A C, Kushwaha R, Prakash O, Ogra R G, Bhattacharya A, Nagar P K and Ahuja P S. 2008. An efficient method of propagation of Podophyllum hexandrum: An endangered medicinal plant of the Western Himalayas under ex situ conditions. Journal of Natural Medicines. 62 (2) 211–216.

38. Kirthikar KR and Basu BD1980 Indian medicinal plants. Vol. 1-4. Bishen Singh Mahendra Pal Singh, Dehradun, India.

39. Kumar S. 2007. Germination and seed storage behaviour in Pongamia pinnata L. Current science. 93(7) 910-911.

40. Kundu M, Tiwari S and Haldkar M. 2020. Collection, germination and storage of seeds of Saraca asoca (Roxb.) Willd. Journal of Applied Research on Medicinal and Aromatic Plants. 16 100231.

41. Lalithkumar B V B. 2008. Standardization of seed testing procedures and storage studies in selected medicinal crops. M.Sc. (Agri.) Thesis, Univ. Agric. Sci., Dharwad (India).

42. Ma Claudia C, Ernesto T, Jessica del Pilar J and Jaqueline B. 2020. Growth and development of Stevia cuttings during propagation with hormones in different months of the year. Plants. 9 294.

43. Madhushree S I, RavirajaShetty G, Souravi K, Rajasekharan P E, Ganapathi M and Ravi C S. 2018. Standardization of seed and vegetative propagation techniques in Saraca asoca (Roxb.) De Wilde: An endangered medicinal plant. International Journal of Current Microbiology and Applied Sciences. 7(04) 1327-1335.

44. Masoodi T H and Masoodi N A. 2000. Germination and growth behaviour of endangered multipurpose tree species- Ulmus wallichiana. Ann. For. 8(1) 45-52.

45. Mathew G, Skaria B P and Joseph A. 2011. Standardization of conventional propagation techniques for four medicinal species of genus Ficus Linn. Indian Journal of Natural Products and Resources. 2(1) 88-96.

46. Misiha A and El-Ashry A. 1991. Seed germination and seedling growth of Magnolia randiflora L. Bull. Fac. Agric. Cairo Univ. 42 (3) 869-879.

47. Mokat D N and Kharat T D. 2022. Essential Oil Composition in Leaves of Ocimum Species Found in Western Maharashtra, India. J. Essent. Oil-Bear. Plants, 25 (1):1 – 8.

48. Mokat D N, Salvi P P, Kamble P N, Awale M S, Kharat T D and Mahadam P D. 2022. Propagation studies on threatened and medicinally important Rauvolfia serpentina (L.) Benth. ex Kurz through stem and root cuttings. International Journal of Botany Studies. 7(6):69-78.

49. Mokat D N, Thorat A P, Mane A V, Desai J S and Bhave S D. 2012. Vegetative propagation in Salacia chinensis L. (Saptarangi). International Journal of forest Usufructs Management. 13(1) 18-21.

50. Murashige T and Skoog F. 1962. A revised medium for rapid growth and bio assays with Tobacco tissue cultures. Physiologia plantarum. 5(3) 473-497.

51. Murthy R and Pullaiah T. 2005. Vegetative propagation of Hemidesmus indicus R.Br. by stem cutting. In Indian Forester.131(11)505-1508.

52. Naidu CV and Mastan M. 2001. Seed pre-treatment methods to improve germination in Pterocarpus santalinus Linn. Indian Journal of Forestry. 24(3) 342-343.

53. Pal S K and Shukla Y. 2003. Herbal Medicine: Current status and the future. Asian Pacific Journal of Cancer Prevention. 4281-288.

54. Pandey A K. 2012. Cultivation technique of an important medicinal plant Gymnema sylvestre R. Br. (Gurmar). Academic Journal of Plant Sciences. 5(3) 84-89.

55. Pascaline J, Charles M, George O, Lukhoba C, Ruth L N and Manani S D. 2010. Ethnobotanical survey and propagation of some endangered medicinal plants from south Nandi district of Kenya. Journal of Animal & Plant Sciences. 8(3) 1016- 1043.

56. Patil H, Tank R V, Bennurmath P and Patel M. 2018. Effect of seed treatment on germination and seedling growth of Jamun (Syzygium cuminii L.) International Journal of Current Microbiology and Applied Sciences. 7(2) 2654-2659.

57. Phatak R S, Hegde N K, Gangadharappa P M and Hegde L. 2017. Effect of seed treatment on germination in Sarpagandha (Rauvolfia serpentina Benth). International Journal of Current Microbiology and Applied Sciences. 6(12) 135-140.

58. Rakshe A T and Mokat D N. 2018. On vegetative propagation through stem cuttings in medicinally lucrative Tinospora species. Journal of Pharmacognosy and Phytochemistry. 7(2) 2313-2318.

59. Ramakrishnan R, Periyasamy R, Lakkakula S, Subramani P, Arockiam S and Manikandan R. 2017. In Vitro propagation and conservation of useful endangered medicinal plants with anticancer activity. Journal of Molecular Biology and Biotechnology. 2 (3)8.

60. Rao P S, Venkaiah K, Murali V and Satyanarayana V V V. 2000. Macro propagation of some important medicinal plants of Andhra Pradesh. Indian Forester. 126 (12) 1265-1269.

61. Rasebeka L, Mathowa T and Mojeremane W. 2014. Effect of Seed Pre-sowing Treatment on Germination of Three Acacia Species Indigenous to Botswana. International Journal of Plant & Soil Science. 3(1)62-70.

62. Reddy B O, Giridhar P and Ravishankar G A. 2001. In vitro rooting of Decalepis hamiltonii Wight Arn., an endangered shrub, by auxins and root promoting agents. Current Science. 81(11) 1479-1482.

63. Renard H A and Cleark P. 1978. Levier de dormance par les gibberellins chez quate especes. Impatience balsamina, Lavendula anguistifolia, Brassica rapa, et viola odorata. Seed Science and Technology. 6 661-677.

64. Salvi P R, Patil R.R and Mokat D N. 2015. Seed Germination Studies in Rauvolfia serpentina (L.) Benth. ex Kurz. European Journal of Medicinal Plants. 10(1)1-7.

65. Sedghi M, Ali N and Esmaielpour B. 2010. Effect of seed priming on germination and seedling growth of two medicinal plants under salinity. Emirates Journal of Food and Agriculture. 22(2) 130-139.

66. Semwal D K, Chauhan A, Kumar A, Aswal S, Semwal B R and Kumar A. 2019. Status of Indian medicinal plants in the International Union for Conservation of Nature and the future of Ayurvedic drugs: Shouldn’t think about Ayurvedic fundamentals? Journal of Integrative Medicine. 17 (4) 238-243.

67. Shankar D. 2019. The FRLHT-TDU story of conservation and sustainable use of medicinal flora. Journal of Medicinal Plant Conservation. 25(1) 45-63.

68. Silva O B, Goelzer A, Santos C C, Torales E P, Zárate N A H and Vieira M C. 2020. Propagation of Aloysia citriodora Palau Using Different Cutting Types under Light Intensity. European Journal of Medicinal Plants. 31(3)1-9.

69. Singh S and Soni S L. 1974. Effect of acid and water soaking periods on seed germination of guava. Punjab Horticultural Journal. 14(3/4) 122-124.

70. Soares A N, Novembre A D L, Martins A R, Piedade S M S and Appezzato da-Glória B. 2011. Propagation studies in Smilax fluminensis Steud. (Smilacaceae).Ciência Rural, Santa Maria 4(10) 1762-1768.

71. Somashekhar B S and Sharma M. 2000. Propagation techniques of commercially important medicinal plants. Training manual on. pp118. Published by Foundation for Revitalisation of Local Health Traditions, Bangalore.

72. Somashekhar B S and Sharma M. 2002. Propagation techniques of commercially important medicinal plants. Published by Foundation for Revitalisation of Local Health Traditions, Bangalore.

73. Srikun N. 2017. In vitro propagation of the aromatic herb Strobilanthes tonkinensis Lindau. Agriculture and Natural Resources. 51 15-19.

74. Srivastava N, Sharma V, Anoop K, Dobriyal Kamal B, Gupta S and Jadon V S. 2011. Influence of pre-sowing treatments on in vitro seed germination of Ativisha (Aconitum heterophyllum Wall) of Uttarakhand. Biotechnology. 10 (2) 215-219.

75. Sudha C G and Seeni S. 2001. Establishment and analysis of fast-growing normal root culture of Decalepis arayalpathra, a rare endemic medicinal plant. Current Science. 81 (4) 371-374.

76. Tewari D N. 2003. Report of the Task Force on Conservation and Sustainable use of Medicinal Plants. Pub. Government of India, Planning Commission. 2000.

77. Vanangamudi K and Vanangamudi M. 2003. Response of Tamarind (Tamarindus indica) to pre-sowing seed treatment with growth stimulants. Journal of Tropical Forest Science. 15(1)6-11.

78. Veeraiah S and Reddy K J. 2012. Current strategic approaches in ethno medicinal plants of Tinospora cordifolia and Gloriosa superba– A review. International Journal of Pharma and Bio Sciences. 3 (2) 320-326.

79. Venkatesh A, Vanangamudi M, Vanangamudi K, Umarani R and Parthiban K T. 1997. Improving seed germination and seedling growth of Pongamia pinnata (L) Pierre through pre-sowing seed treatment. Journal of Tree Science. 16 75–80.

80. Vijendra P D, Jayannaa S G, Kumar V, Gajula H, Rajashekar J, Torankumar S, Basappa G and Anuradha C M. 2017. Rapid in vitro propagation of Lucas aspera Spreng. A potential multipurpose Indian medicinal herb. Industrial Crops & Products. 107 281–287.

81. Waman A A, Smitha G R and Bohra P. 2019. A review on clonal propagation of medicinal and aromatic plants through stem cuttings for promoting their cultivation and conservation. Current Agriculture Research Journal. 7(2)122-138.

82. Wilson P J. 1993. Propagation characteristics of Eucalytus globules Labill. Spp. globules stem cutting in relation to their original position in the parent shoot. Journal of Horticultural Sciences. 68(5)715-724.

83. Yadav P K. 2019. Trade in Medicinal and Aromatic plants of India: An overview. TRAFFIC’s newsletter. 31 7-16.

Submitted

2025-12-27

Published

2026-04-10

Issue

Section

Review Article

How to Cite

Kharat, T., & Mokat, D. (2026). Advances in propagation and conservation of medicinal plant species: A review. The Indian Journal of Agricultural Sciences, 96(4). https://doi.org/10.56093/ijas.v96i4.174617
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