Detection of Meloidogyne graminicola (RRKN) in Kumaon region of the Indian Himalayas

ASHISH KUMAR SINGH; AMIT U PASCHAPUR; AMAR KANT KUSHWAHA; K K MISHRA; LAKSHMI KANT

doi:10.56093/ijas.v93i7.135351

Authors

ASHISH KUMAR SINGH ICAR-Vivekanada Parvatiya Krishi Anusandhan Sansthan, Almora, Uttarakhand 263 601, India
AMIT U PASCHAPUR ICAR-Vivekanada Parvatiya Krishi Anusandhan Sansthan, Almora, Uttarakhand 263 601, India
AMAR KANT KUSHWAHA ICAR-Central Institute for Subtropical Horticulture, Lucknow, Uttar Pradesh
K K MISHRA ICAR-Vivekanada Parvatiya Krishi Anusandhan Sansthan, Almora, Uttarakhand 263 601, India
LAKSHMI KANT ICAR-Vivekanada Parvatiya Krishi Anusandhan Sansthan, Almora, Uttarakhand 263 601, India

https://doi.org/10.56093/ijas.v93i7.135351

Keywords:

Distribution patterns, Kumaon hills, Meloidogyne graminicola, Rice, Surveillance

Abstract

Rice root-knot nematode (RRKN), Meloidogyne graminicola is a serious pest of rice (Oryza sativa L.) reported globally. In India, RRKN has been reported from various parts of the rice growing region and causing 17–30% yield loss. In the present study, RRKN population infesting rice growing region of Kumaon hills of Uttarakhand, Indian Himalayan region was isolated during 2019–20, characterized using integrative taxonomy by combining molecular analysis using ITS, morphology of male, juveniles, adult female and morphometry measurements of different stages. The data presented in the comparative analysis of morphometry parameters for Meloidogyne graminicola isolate from the Kumaon region of the Indian Himalayas, as compared to the original description by Golden and Birchfield (1965) from Louisiana, USA, reveals some differences in the morphological characteristics of adult females, adult males, and 2nd stage juveniles (J2) of M. graminicola. The ITS sequence from the present study appeared as a single clade between the Fujian isolate (KM111531.1) and the Brazilian M. graminicola isolate (KY962651). To our knowledge, this is the first authentic characterization and report of RRKN from the Hilly region of Uttarakhand state. The detection of M. graminicola in the mid Indian Himalayan region provides valuable information for further studies on the natural population dynamics and distribution patterns of this nematode species. The findings highlight the need for regular monitoring and surveillance of nematode populations in the region to better understand their distribution and potential impact on rice production.

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References

Bogale M, Baniya A and DiGennaro P. 2020. Nematode identification techniques and recent advances. Plants 9(10): 1260. DOI: https://doi.org/10.3390/plants9101260

Da Silva Mattos V, Cares J, Gomes C, Almeida M R, Gomes A C, Monteiro J and Carneiro R. 2017. First report of, and additional information on, Meloidogyne oryzae (Nematoda: Meloidogyninae) parasitizing rice crop in South of Brazil. NCBI KY962651.1

Dutta T K, Ganguly A K and Gaur H S. 2012. Global status of rice root-knot nematode, Meloidogyne graminicola. African Journal of Microbiology Research 6(31): 6016–21. DOI: https://doi.org/10.5897/AJMR12.707

Fanelli E, Cotroneo A, Carisio L, Troccoli A, Grosso S, Boero C and De Luca F. 2017. Detection and molecular characterization of the rice root-knot nematode Meloidogyne graminicola in Italy. European Journal of Plant Pathology 149(2): 467–76. DOI: https://doi.org/10.1007/s10658-017-1196-7

Felsenstein J. 1985. Confidence limits on phylogenies: An approach using the bootstrap. Evolution 39: 783–91. DOI: https://doi.org/10.1111/j.1558-5646.1985.tb00420.x

Gaur H S. 2023. Root-knot nematode disease: an expanding problem in rice-based cropping systems. Integrated Pest Management in Diverse Cropping Systems, pp. 183–208. Apple Academic Press. DOI: https://doi.org/10.1201/9781003304524-7

Golden A M and Birchfield W. 1965. Meloidogyne graminicola (Heterodera), a new species of root knot nematode from grass. Proceedings of the Helminthological Society of Washington 32: 228–31.

Hartman K M and Sasser J N. 1985. Identification of Meloidogyne species on the basis of differential host test and perineal pattern morphology. An advanced treatise on Meloidogyne 2: 69–77.

Jain R K, Khan M R and Kumar V. 2012. Rice root-knot nematode (Meloidogyne graminicola) infestation in rice. Archives of Phytopathology and Plant Protection 45(6): 635–45. DOI: https://doi.org/10.1080/03235408.2011.588059

Jaiswal R K, Singh K P and Srivastava A N. 2011. Pathogenic effect of Meloidogyne graminicola on growth of rice seedlings and susceptibility of cultivars. Annals of Plant Protection Sciences 19(1): 174–77.

Jones J T, Haegeman A, Danchin E G, Gaur H S, Helde J and Jones M G. 2013. Top 10 plant-parasitic nematodes in molecular plant pathology. Molecular Plant Pathology 14(9): 946–61. DOI: https://doi.org/10.1111/mpp.12057

Khan M R and Ahamad F. 2020. Incidence of root-knot nematode (Meloidogyne graminicola) and resulting crop losses in paddy rice in northern India. Plant Disease 104(1): 186–93. DOI: https://doi.org/10.1094/PDIS-12-18-2154-RE

Kumar S, Stecher G, Li M, Knyaz C and Tamura K. 2018. MEGA X: molecular evolutionary genetics analysis across computing platforms. Molecular Biology and Evolution 35: 1547–49. DOI: https://doi.org/10.1093/molbev/msy096

Kumar V, Khan M R and Walia R K. 2020. Crop loss estimations due to plant-parasitic nematodes in major crops in India. National Academy Science Letters 43: 409–12. DOI: https://doi.org/10.1007/s40009-020-00895-2

Prasad J S, Vishakanta and Gubbaiah. 2001. Outbreak of root knot nematode, Meloidogyne graminicola in Mandya district, Karnataka state and farmers' perceptions. (In) National Congress on Centenary of Nematology in India - Appraisal and Future Plans, New Delhi, December 5–7, 2001.

Pokharel R R, Abawi G S, Duxbury J M, Smat C D, Wang X and Brito J A. 2010. Variability and the recognition of two races in Meloidogyne graminicola. Australasian Plant Pathology 39: 326–33. DOI: https://doi.org/10.1071/AP09100

Singh P, Mishra K K and Patnaik A. 2019. Harnessing the powerful potential of genomics in nematology. (In) National Seminar on "Recent Advances in Agriculture for Sustainable Rural Development, BAU Bihar, March, ISBN: 978-93-85675-11-9.

Tamura K and Nei M. 1993. Estimation of the number of nucleotide substitutions in the control region of mitochondrial DNA in humans and chimpanzees. Molecular Biology and Evolution 10: 512–26.

Tian Z L, Maria M, Barsalote E M, Castillo P and Zheng J W. 2018. Morphological and molecular characterization of the rice root-knot nematode, Meloidogyne graminicola, Golden and Birchfield, 1965 occurring in Zhejiang, China. Journal of Integrative Agriculture 17(12): 2724–33. DOI: https://doi.org/10.1016/S2095-3119(18)61971-9

Torrini G, Roversi P F, Cesaroni C F and Marianelli L. 2020. Pest risk analysis of rice root-knot nematode (Meloidogyne graminicola) for the Italian territory. EPPO Bulletin 50(2): 330–39. DOI: https://doi.org/10.1111/epp.12666

Zhou X, Liu G K, Xiao S and Zhang S S. 2015. First report of Meloidogyne graminicola infecting banana in China. Plant Disease 99(3): 420. DOI: https://doi.org/10.1094/PDIS-08-14-0810-PDN