Incidence and epidemiology of rice (Oryza sativa) false smut caused by Ustilaginoidea virens in northern India

PRAHLAD  MASURKAR; RAKESH KUMAR  SINGH; MANAS KUMAR  BAG; HAUSILA PRASAD  SINGH; MOHAMMAD EKRAM  HUSSAIN

doi:10.56093/ijas.v93i12.136310

Authors

PRAHLAD MASURKAR Institute of Agricultural Sciences, Banaras Hindu University, Varanasi, Uttar Pradesh 221 005 India
RAKESH KUMAR SINGH Institute of Agricultural Sciences, Banaras Hindu University, Varanasi 221005 India
MANAS KUMAR BAG ICAR-National Rice Research Institute, Cuttack 753006
HAUSILA PRASAD SINGH BRD PG College, Deoria, 274001 India
MOHAMMAD EKRAM HUSSAIN Centre for Plant Breeding and Genetics, Tamil Nadu Agricultural University, Coimbatore, 641003

https://doi.org/10.56093/ijas.v93i12.136310

Keywords:

False smut, ITS markers, Prevalence, Varieties, Weather

Abstract

False smut of rice (Oryza sativa L.) caused by Ustilaginoidea virens (Cooke) Takah., 1896 is an important emerging disease of rice. An experiment was conducted during 2018–19 and 2019–20 at Institute of Agricultural Sciences, Banaras Hindu University, Varanasi, Uttar Pradesh to study the U. virens prevalence and morphological variability in northern India with the effect of weather variables on the percentage disease incidence (PDI) of false smut of rice. The study revealed that the false smut incidence was higher in Varanasi district of Uttar Pradesh (53.64%). The lowest percentage (20.68–27.55%) of infected tillers was found in Kangra district of Himachal Pradesh, and Tehri Garhwal district of Uttarakhand. The grain replacement percentage was highest in the Moti cultivar (51.17%), while the highest yield loss percentage was in the Pusa Basmati (57.11 %) variety. The majority of the isolates in our investigation had fluffy, raised, and irregular colony morphology. The correlation between the false smut disease incidence and weather has been evaluated in the Varanasi district, and found that the temperature of 20.36°C with an RH of 85.13% and low rainfall of 8.92 mm was conducive to disease initiation and positively correlated with the PDI. Other epiphytotic factors like sunshine hours, and wind speed were all found to be negatively linked with PDI. In this investigation, for the first time, the viability of spores was also checked. It was found that 2-year-old spores were not viable because they did not retain any Brilliant Cresyl blue colour dye, which stains the stored food material in spores, while 1-month-old spores were found to be 100% viable. All the isolates were further confirmed with the specific ITS marker.

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References

Alace S, Nagaraja A, Pramesh D and Prasanna Kumar M K. 2021. Influence of weather parameters on false smut disease development in rice. Mysore Journal of Agriculture Science 55(4): 320–25.

Anbazhagan P, Theradimani M, Ramamoorthy V, Vellaikumar P and Oviya R. 2022. Survey and surveillance on rice false smut disease severity in Tamil Nadu and the influence of wind velocity on disease progression under field conditions. Journal of Applied and Natural Science 143: 938–45.

Bag M K, Ray A, Masurkar P, Devanna B N, Parameswaran C, Baite M, Rath P C and Nayak A. 2021. Genetic diversity and population structure analysis of isolates of the rice false smut pathogen Ustilaginoidea virens in India. Plant Pathology 705: 1085–97.

Bag M K, Yadav M K and Mukherjee A K. 2017. Changing disease scenario with special emphasis on false smut of rice. SATSA Mukhapatra-Annual Technical Issue 21: 219–24.

Baite M S, Raghu S, Lenka S, Mukherjee A K, Prabhukarthikeyan S R and Jena M. 2017. Survey of rice false smut caused by Ustilaginoidea virens in Odisha. The Bioscan 12: 2081–85.

Baite M S, Raghu S, Prabhukarthikeyan S R, Mukherjee A K, Bag M K, Lenka S and Jena M. 2019. Yield loss assessment in rice Oryza sativa due to false smut infection. The Indian Journal of Agricultural Sciences 90(2): 361–64.

Boedijn K B. 1956. Trypan blue as a stain for fungi. Stain Technology 31: 115–16.

Fan J, Yang J, Wang Y Q, Li G B, Li Y, Huang F and Wang W M. 2016. Current understanding on Villosiclava virens a unique flower-infecting fungus causing rice false smut disease. Molecular Plant Pathology 179: 1321–30.

Husson O, Tano B F and Saito K. 2022. Designing low-input upland rice-based cropping systems with conservation agriculture for climate change adaptation: A six-year experiment in Mbe Bouake Cote d’Ivoire. Field Crops Research 277: 108418.

Jaglan M S, Chaudhary O P, Ahlawat S and Yadav J. 2023. Bio- efficacy of chlorantraniliprole 0.4% GR against Cnaphalocrocis medinalis and Scirpophaga incertulas in rice (Oryza sativa). The Indian Journal of Agricultural Sciences 93(2): 157–62. https://doi.org/10.56093/ijas.v93i2.119318

Jia Q, Lv B, Guo M, Luo C, Zheng L, Hsiang T and Huang J. 2015. Effect of rice growth stage temperature relative humidity and wetness duration on infection of rice panicles by Villosiclava virens. European Journal of Plant Pathology 141: 15–25.

Ladhalakshmi D, Laha G S, Singh R, Karthikeyan A, Mangrauthia S K, Sundaram R M, Thukkaiyannan P and Viraktamath B C. 2012. Isolation and characterization of Ustilaginoidea virens and survey of false smut disease of rice in India. Phytoparasitica 40: 171–76.

Ladhalaxmi D, Madamsetty S P, Vellaichamy P, Donempudi K, Banda S, Singh R, Prasad V, Lore J S, Jain J, Mariappan S and Laha G S. 2019. Geographic distribution of false smut disease of rice in India and efficacy of selected fungicides for its management. International Journal of Pest Management 65(2): 177–85.

Lindemann U, Helleman S, Filippova N, Krieglsteiner L and Pennanen M. 2014. Micropeziza curvatispora sp nov M fenniae sp nov and M zottoi sp nov Helotiales–three new species of the genus Micropeziza from Western Siberia Finland Germany and Belgium.

Masurkar P, Bag M K, Ray A, Singh R K, Baite M S and Rath P C. 2022. Genetic diversity and population structure analysis of rice false smut pathogen in North India using molecular markers. Journal of Phytopathology 170(2): 124–40. https:// doiorg/101111/JPH13061

Raji P, Sumiya K V, Renjisha K, Dhanya S and Narayanankutty M C. 2016. Evaluation of fungicides against false smut of rice caused by Ustilaginoidea virens. International Journal of Applied and Natural Sciences 52: 77–82.

Rush M C, Shahjahan A K M Jones J P and Groth D E. 2000. Outbreak of false smut of rice in Louisiana. Plant Diseases 84: 100.

Saha M, Chakraborty A and Bhattacharya K. 2020. Aerobiology epidemiology and disease forecasting of false smut disease of rice in West Bengal India. Aerobiologia 36(2): 299–304.

Sanghera G S, Ahanger M A, Kashyap S C, Bhat Z A, Rather A G and Parray G A. 2012. False smut of rice Ustilaginoidea virens under temperate agro-climatic conditions of Kashmir India. Elixir Biology Technique 49: 9827–31.

Sarker M M, Haque A H M M, Nessa B, Salam M U, Islam M M and Muqit A. 2016. Status of rice false smut disease in Natore district of Bangladesh. Bangladesh Rice Journal 202: 31–37.

Singh A K and Pophaly D J. 2010. An unusual rice false smut epidemic reported in Raigarh district Chhattisgarh India. International Rice Research Notes 35: 1–3.

Singh H P, Basandrai D, Rathour R and Basandrai A K. 2021. Inheritance and allelic relationship between genes conferring resistance to Pyricularia oryzae in rice. Indian Phytopathology 1–8.