Cultural, morphological and molecular variability in Fusarium moniliformeisolates causing bakanae disease of aromatic rice in Haryana, India

VIKRAM  SINGH; ASHWANI  KUMAR; PROMIL  KAPOOR; MAHAVEER SINGH  BOCHALYA; RAKESH  KUMAR

doi:10.56093/ijas.v95i6.137127

Authors

VIKRAM SINGH Chaudhary Charan Singh Haryana Agricultural University, Hisar, Haryana 125 004, India
ASHWANI KUMAR Chaudhary Charan Singh Haryana Agricultural University, Hisar, Haryana 125 004, India
PROMIL KAPOOR Chaudhary Charan Singh Haryana Agricultural University, Hisar, Haryana 125 004, India
MAHAVEER SINGH BOCHALYA Chaudhary Charan Singh Haryana Agricultural University, Hisar, Haryana 125 004, India
RAKESH KUMAR Chaudhary Charan Singh Haryana Agricultural University, Hisar, Haryana 125 004, India

https://doi.org/10.56093/ijas.v95i6.137127

Keywords:

Aromatic rice, Bakanae, Cultural variability, Fusarium moniliforme, Molecular variability

Abstract

Bakanae disease caused by Fusarium moniliforme has become a serious problem in the successful cultivation of aromatic rice under north-west plain zone of India. The present study was carried out during rainy (kharif) season of 2020 and 2021 at Chaudhary Charan Singh Haryana Agricultural University, Hisar, Haryana to assess cultural, morphological and molecular variability among 26 selected isolates of Fusarium moniliforme collected from different locations and 11 districts of Haryana from roving survey conducted which includes bakanae infected plants of aromatic rice varieties namely PB 1121, PB 1718, PB 1401, PB 1509 and Basmati 521. On potato dextrose agar (PDA) media, colony colour of different isolates varied from white, milky white to creamy white. The microscopic observations showed that only three isolates showed macro-conidia, whereas the others 23 had micro-conidia. The macro and micro conidia size ranged between (22.36–28.09) × (3.01–4.71) and (4.80–9.92) × (1.42–2.71) μm in length and breadth, respectively. The isolates were divided into 3 groups based on cultural characteristics, macro and micro conidial size range. The precision molecular characterization of different isolates was done using specific primers, viz. β-tubulin, Vertf and (rp 32 and rp 33) primers which were found positive for 20, 22 and 9 isolates, respectively. The present investigations confirm that, there is variability among 26 isolates on the basis of morphological characteristics and further precision technique molecular markers confirm the same in Fusarium moniliforme causing bakanae disease and they may be useful in breeding programme for bakanae resistant genotypes.

Downloads

Download data is not yet available.

References

Amoah B K, Rezanoor H N, Nicholson P and Mac-Donald M V. 1995. Variation in the Fusarium section Liseola: Pathogenicity and genetic studies of Fusarium moniliforme Sheldon from different hosts in Ghana. Plant Pathology 44: 563–72.

Aris A, Hasan Z A E, Shohaimi S, Saidi N B and Zainudin N A I M. 2020. Morphological, phylogenetic and pathogenicity characterisation of Fusarium species associated with wilt disease of pumpkin (Cucurbita pepo Linnaeus). Asian Journal of Agriculture and Biology 8(1): 75–84.

Asmaul H, Asaduzzaman M M and Nor N M I M. 2020. Rice bakanae disease: an emerging threat to rice production in Bangladesh. Asian Journal of Medical and Biological Research 6(4): 608–10.

Bag D, Khilari K, Yadav A K, Singh A and Singh P. 2022. Morphological and molecular characterization of Fusarium spp. causing Bakanae disease of rice (Oryza sativa) in western Uttar Pradesh, India. The Indian Journal of Agricultural Sciences 92(5): 582–86.

Bashyal B M, Aggarwal R, Rawat K, Sharma S, Gupta A K, Choudhary R, Bhat J, Krishnan S G and Singh A K. 2020. Genetic diversity and population structure of Fusarium fujikuroi causing Bakanae, an emerging disease of rice in India. Indian Journal of Experimental Biology 58(1): 45–52.

Bashyal B M, Aggarwal R, Sharma S, Gupta S, Rawat K, Singh D, Singh A K and Gopala K S. 2015. Occurrence, identification and pathogenicity of Fusarium species associated with bakanae disease of basmati rice in India. European Journal of Plant Pathology 144: 457–66.

Desjardins A E, Manandhar H K, Plattner R D, Manandhar G G, Poling S M and Maragos C M. 2000. Fusarium species from Nepalese rice and production of mycotoxins and gibberellic acid by selected species. Applied and Environment Microbiology 66(3): 1020–25.

Egerci Y, Teksür P K and Morca A U. 2022. Identification of Fusarium andiyazi associated with the bakanae disease of rice in Turkey. Current Microbiology 79(10): 291.

Glass N L and Donaldson G C. 1995. Development of primer sets designed for use with the PCR to amplify conserved genes from filamentous ascomycetes. Applied and Environmental Microbiology Journal 61: 1323–30.

Gupta A K, Singh Y, Jain A K and Singh D. 2014. Prevalence and incidence of bakanae disease of rice in northern India. Journal of Agriculture Search 1(4): 233–37.

Jeon Y A, Yu, S H, Lee, Y Y, Park, H J, Lee S, Sung J S, Kim Y G and Lee H S. 2013. Incidence, molecular characteristics and pathogenicity of Gibberella fujikuroi species complex associated with rice seeds from Asian countries. Mycobiology 41(4): 225–33.

Leslie J F and Summerell B A. 2006. The Fusarium Laboratory Manual, pp. 388. Blackwell Publishing Ltd, UK.

Mohiddin F A, Majid R, Bhat A H, Dar M S, Shikari A B, Sofi N

R, Nabi S U, Hamid A, Ahanger M A, Bhat F A and Hussain

A. 2021. Molecular phylogeny, pathogenic variability and phytohormone production of Fusarium species associated with bakanae disease of rice in temperate agro-ecosystems. Molecular Biology Reports 48: 3173–84.

Murray M G and Thompson W F. 1980. Rapid isolation of high molecular weight plant DNA. Nucleic Acids Research 8: 4321–25.

Nosratabadi M, Kachuei R, Rezaie, S and Harchegani A B. 2018. Beta-tubulin gene in the differentiation of Fusarium species by PCR-RFLP analysis. Infez Med 26(1): 52–60.

Ouattara Y K, Kouamé-Sina S M, Coulibaly K J, Casimir B Y, Beni C K, Sabine V N, Touré A O and Dadié A. 2021. Detection of a gene Involved in fumonisin production in Fusarium strains isolated from Moringa (Moringa oliefera) sold in the markets of Abidjan (Ivory Coast). American Journal of Microbiological Research 9(3): 96–102.

Patino B, Mirete S, Ganzalez T, Mule G, Rodriguez T M and Vazquez C. 2004. PCR detection assay of fumonisin-producing Fusarium verticillioides strains. Journal of Food Protection 6: 1278–83.

Proctor R H, Brown D W, Plattner R D, and Desjardins A E. 2003. Coexpression of 15 contiguous genes delineates a fumonisins biosynthetic gene cluster in Gibberella moniliformis. Journal of Fungal Genetics and Biology 38: 237–49.

Procter R H, Plattner R D, Brown D W, Seo J A and Lee Y W. 2004. Discontinuous distribution of fumonisin biosynthetic genes in the Gibberella fujikuroi species complex. Mycological Research 108(7): 815–22.

Puyam A, Pannu P P S, Kaur J and Sethi S. 2017. Cultural, morphological and molecular variability of Fusarium moniliforme Sheld. causing foot rot disease of basmati rice in Punjab. Journal of Mycology and Plant Pathology 47(4): 369–81.

Qiu J, Lu Y, He D, Lee Y W, Ji F, Xu J and Shi J. 2020. Fusarium fujikuroi species complex associated with rice, maize and soybean from Jiangsu Province, China: Phylogenetic, pathogenic and toxigenic analysis. Plant Disease 104(8): 2193–2201.

Singh R and Sunder S. 2012. Foot rot and bakanae of rice: an overview. Review of Plant Pathology 5: 566–604.

Zidan L, Jawdat D and Naffaa W. 2020. Morphology, pathogenicity and molecular identification of some Fusarium species within the Gibberella fujikuroi species complex from wheat in Syria. Current Research in Environmental and Applied Mycology 10: 156–166.