Genetic diversity and mating-type distribution within populations of Ascochyta rabiei in Turkey

GÖKSEL  ÖZER; HARUN BAYRAKTAR; FATMA SARA DOLAR

doi:10.56093/ijas.v82i1.13850

Authors

GÖKSEL ÖZER Ankara University, Faculty of Agriculture, Department of Plant Protection, Ankara 06110
HARUN BAYRAKTAR Ankara University, Faculty of Agriculture, Department of Plant Protection, Ankara 06110
FATMA SARA DOLAR Ankara University, Faculty of Agriculture, Department of Plant Protection, Ankara 06110

https://doi.org/10.56093/ijas.v82i1.13850

Keywords:

Ascochyta rabiei, Chickpea, Genetic diversity, Mating type, RAPD

Abstract

Genetic diversity and mating-type distribution were studied within five populations of Ascochyta rabiei in Turkey. Of 122 isolates tested for mating type, 52.5% were identified as MAT 1-2 and 47.5% as MAT 1-1. Both mating types were observed in all populations, supporting the hypothesis of a randomly mating population. RAPD analysis was performed on 81 isolates of A. rabiei representing different pathotypes and geographic origins with 54 primers. Results of RAPD analysis clustered all isolates into nine groups an arbitrary level of 73% similarity. Total gene diversity (HT=0.221) was mostly attributable to diversity within populations (HS=0.213). Only 4% of the total variability were attributable to variation among populations. The results revealed low genetic differentiation and high gene flow among populations of A. rabiei in Turkey.

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References

Armstrong C L, Chongo G, Gossen B D and Duczek L J. 2001. Mating type distribution and incidence of the teleomorph of Ascochyta rabiei (Didymella rabiei) in Canada. Canadian Journal of Plant Pathology 23: 110–3.

Barve M P, Arie T, Salimath S S, Muehlbauer F J and Peever T L. 2003. Cloning and characterization of the mating type (MAT) locus from Ascochyta rabiei (teleomorph: Didymella rabiei) and a MAT phylogeny of legumes-associated Ascochyta spp. Fungal Genetics and Biology 39: 151–67.

Bayraktar H, Dolar F S and Tör M. 2007a. Determination of genetic diversity within Ascochyta rabiei (Pass.) Labr., the pathogen of ascochyta blight of chickpea in Turkey. Journal of Plant Pathology 89: 341–7.

Bayraktar H, Dolar F S and Maden S. 2007b. Mating type groups of Ascochyta rabiei (teleomorph: Didymella rabiei), the causal agent of chickpea blight in Central Anatolia. Turkish Journal of Agriculture and Forestry 31: 41–6.

Chongo G, Gossen B D, Buchwaldt L, Adhikari T and Rimmer S R. 2004. Genetic diversity of Ascochyta rabiei in Canada. Plant Disease 88: 4–10.

Kaiser W J and Küsmeno lu I. 1997. Distribution of mating types and the teleomorph of Ascochyta rabiei on chickpea in Turkey. Plant Disease 81: 1284–7.

Milgroom M G. 1996. Recombination and the multilocus structure of fungal populations. Annual Review of Phytopathology 34: 457–77.

Miller M P. 1997. Tools for Population Genetic Analysis. Version 1.3. Department of Biological Sciences, Northern Arizona University, Flagstaff, AZ.

Peever T L, Salimath S S, Su G, Kaiser W J and Muehlbauer F J. 2004. Historical and contemporary multilocus population structure of Ascochyta rabiei (teleomorph: Didymella rabiei) in the Pacific Northwest of the United States. Molecular Ecology 13: 291–309.

Phan H T T, Ford R and Taylor P W J. 2003. Population structure of Ascochyta rabiei in Australia based on STMS fingerprints. Fungal Diversity 13: 111–29.

Rhaiem A, Cherif M, Dyer P S and Peever T L. 2007. Distribution of mating types and genetic diversity of Ascochyta rabiei populations in Tunisia revealed by mating-type specific PCR and random amplified polymorphic DNA (RAPD) markers. Journal of Phytopathology 155: 596–605.

Rhaiem A, Cherif M, Peever T L and Dyer P S. 2008. Population structure and mating system of Ascochyta rabiei in Tunisia: evidence for the recent introduction of mating type 2. Plant Pathology 57: 540–51.

Rohlf F I. 1998. NTSYS-pc. Numerical Taxonomy and Multivariate Analysis System. Version 2.0. Applied Biostatistics, New York, USA.

Santra D K, Singh G, Kaiser W J, Gupta V S, Ranjekar P K and Muehlbauer F J. 2001. Molecular analysis of Ascochyta rabiei (Pass.) Labr., the pathogen of Ascochyta blight in chickpea. Theoretical and Applied Genetics 102: 676–82.

Trapero-Casas A and Kaiser W J. 1992. Development of Didymella rabiei, the teleomorph of Ascochyta rabiei, on chickpea straw. Phytopathology 82: 1261–6.

Türkan M and Dolar F S. 2009. Determination of pathogenic variability of Didymella rabiei, the agent of ascochyta blight of chickpea in Turkey. Turkish Journal of Agriculture and Forestry 33: 585–91.

Udupa S, Weigand F, Saxena M and Kahl G. 1998. Genotyping with RAPD and microsatellite markers resolves pathotype diversity in the ascochyta blight pathogen of chickpea. Theoretical and Applied Genetics 97: 299–307.

Weising K, Kaemmer D, Epplen J T, Weigand F, Saxena M and Kahl G. 1991. DNA fingerprinting of Ascochyta rabiei with synthetic oligodeoxynucleotides. Current Genetics 19: 483–9.

Wilson A D and Kaiser W J. 1995. Cytology and genetics of sexual incompatibility in Didymella rabiei. Mycologia 87: 795–804.

Yeh F C, Yang R C, Boyle T J, Ye Z H and Mao J X. 1999. Popgene, the user-friendly shareware for population genetic analysis. Molecular Biology and Biotechnology Centre, University of Alberta, Edmonton, Canada.