Comparative effect of auxin analogues on induction of polyhaploids in triticale and triticale wheat hybrids through wheat maize system

ADITYA PRATAP; HARINDER K CHAUDHARY

doi:10.56093/ijas.v82i1.13879

Authors

ADITYA PRATAP CSK Himachal Pradesh Agricultural University, Palampur 176 062
HARINDER K CHAUDHARY CSK Himachal Pradesh Agricultural University, Palampur 176 062

https://doi.org/10.56093/ijas.v82i1.13879

Keywords:

Auxins, Chromosome elimination, Haploids, Picloram, 2, 4-D, Triticale Ã— wheat hybrids

Abstract

The study was designed to evaluate two auxins, 2,4-D and picloram for their effectiveness for induction of polyhaploids in triticale and triticale × wheat crosses and to standardize their optimum dose. Ten F₁ and parental genotypes pollinated with maize were subjected to in vivo auxin treatment at three concentrations (100, 250 and 400 mg/l) by injecting at the tiller base of the crossed spikes so as to fill the hollow of their uppermost internode. Both auxins restulted in formation of embryo-carrying seeds, though the performance differed significantly for the three parameters of haploid induction at different concentrations. The highest seed formation, embryo formation, and regeneration frequencies in 2,4-D were recorded at 250, 400 and 250 mg/ l concentration, respectively, whereas, 400, 400 and 250 mg/ l concentration of picloram, respectively, were best for above traits. All the genotypes led to regeneration of haploid plants at 250 mg/l of both the auxins. On the basis of the three haploid induction parameters and overall success rates, the 250 mg/l concentration of both auxins was optimum.

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