A simple modified method of DNA extraction from seeds for PCR amplifications

RAMWANT GUPTA; U S CHANDRASHEKAR; S K CHAKRABARTY; M DADLANI

doi:10.56093/ijas.v82i1.13882

Authors

RAMWANT GUPTA Indian Agricultural Research Institute, New Delhi 110 012
U S CHANDRASHEKAR Indian Agricultural Research Institute, New Delhi 110 012
S K CHAKRABARTY Indian Agricultural Research Institute, New Delhi 110 012
M DADLANI Indian Agricultural Research Institute, New Delhi 110 012

https://doi.org/10.56093/ijas.v82i1.13882

Keywords:

DNA, Genetic purity, PCR, Seed lots

Abstract

Rapid and reliable protocols for testing genetic purity of seed are an important requirement for quality assurance. This method was applied to Indian mustard, rice, wheat and cotton. Yield and quality of DNA was good for PCR analysis. DNA yield ranged from 16.4 to 76.22 ng/mg seed depending on plant species and varieties. The A260/280 ratio ranged from 1.65-1.91, indicating that the isolated DNA was fairly clean and free from protein and RNA contaminations. By adopting this method nearly 200–300 sample can be extracted in 24 hr, which will be useful for assessment of genetic purity of seed lots during seed multiplication as well as in breeding programmes that requires rapid screening of large population.

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