Genetic variability and association study of SSR markers for yield and powdery mildew disease in pea (Pisum sativum)

PHEIRIM  REGINAH; KONJENGBAM NOREN  SINGH; T  RAJESH; MAHANTA  MAYURAKSHEE; SAMUTHIRAPANDI  S; ANDREAN ALLWIN  LYNGDOH; THEMMEICHON  CHAMROY

doi:10.56093/ijas.v94.i3.140129

Authors

PHEIRIM REGINAH College of Post-Graduate Studies in Agricultural Sciences (Central Agricultural University, Imphal, Manipur), Umiam, Meghalaya 793 103, India
KONJENGBAM NOREN SINGH College of Post-Graduate Studies in Agricultural Sciences (Central Agricultural University, Imphal, Manipur), Umiam, Meghalaya 793 103, India
T RAJESH College of Post-Graduate Studies in Agricultural Sciences (Central Agricultural University, Imphal, Manipur), Umiam, Meghalaya 793 103, India
MAHANTA MAYURAKSHEE College of Post-Graduate Studies in Agricultural Sciences (Central Agricultural University, Imphal, Manipur), Umiam, Meghalaya 793 103, India
SAMUTHIRAPANDI S College of Post-Graduate Studies in Agricultural Sciences (Central Agricultural University, Imphal, Manipur), Umiam, Meghalaya 793 103, India
ANDREAN ALLWIN LYNGDOH College of Post-Graduate Studies in Agricultural Sciences (Central Agricultural University, Imphal, Manipur), Umiam, Meghalaya 793 103, India
THEMMEICHON CHAMROY Lovely Professional University, Phagwara, Punjab

https://doi.org/10.56093/ijas.v94.i3.140129

Keywords:

Genetic analysis, Pisum sativum, Powdery mildew, Yield

Abstract

Makhyatmubi, one of the most popular local varieties of field pea (Pisum sativum L.) in Manipur has morpho- physiological bottlenecks such as tall nature and high susceptibility to fungus Erysiphe pisi causing powdery mildew disease. Therefore, an experiment was conducted during winter (rabi) seasons of 2018–19 and 2020–21 at College of Post-Graduate Studies in Agricultural Sciences (Central Agricultural University, Imphal, Manipur), Umiam, Meghalaya to generate genetic variability by hybridization of Makhyatmubi with Prakash variety which is a well-known powdery mildew resistant variety in India and possesses several contrasting characters. Fruitful heterosis was estimated with highest record in number of pods per plant (114.75%) and seed yield per plant (108.04%). These two characters also showed high PCV, GCV, heritability and GAM. Number of pods per plant (0.95) was found to have highest positive significant correlation with seed yield per plant. MP-5 and MP-119 recorded superior performance in several characters under study. For the association study of simple sequence repeats (SSR) markers, easily scorable polymorphic markers were selected for surveying association with the traits. Results revealed that SSR marker AA122 may be associated with seed yield and number of pods per plant while marker AA136 with number of seeds per pod. A5 marker was found to be associated with the powdery mildew disease. Hence, it can be employed for marker assisted selection (MAS) to identify and select pea genotypes exhibit resistance to powdery mildew disease.

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References

Abdel-Rahman M M, Bayoumi S R and Barakat M N. 2016. Identification of molecular markers linked to Fusarium ear rot genes in maize plants Zea mays L. Biotechnology and Biotechnological Equipment 30(4): 692–99.

Bobkov S V and Selikhova T N. 2021. Introgession of powdery mildew resistance into cultural pea from wild accession of P. fulvum. IOP Conference Series: Earth and Environmental Science 650: 012091.

Burstin J, Gallardo K, Mir R R, Varshney R K and Duc G. 2011. Improving protein content and nutrition quality. Biology and Breeding of Food Legumes, pp. 314–28. Pratap A and Kumar J (Eds). Wallingford, CT: CAB International.

Cazzola F, Bermejo C J and Cointry E. 2020. Transgressive segregations in two pea F2 populations and their respective F2:3 families. Pesquisa Agropecuária Brasileira 55: e01623.

Dixon G R. 1987. Powdery mildew of vegetables and allied crops. Powdery Mildew. Speaure D M (Ed). Academic Press, San Diego.

Ek M, Eklund M, Von P R, Dayteg C, Henriksson T, Weibull P, Ceplitis A, Isaac P and Tuvesson S. 2005. Microsatellite markers for powdery mildew resistance in pea (Pisum sativum L.). Hereditas 142: 86–91.

ICAR-IIPR. 2020. ICAR-Indian Institute of Pulses Research. http://dalhangyanmanch.res.in/eng/content_desc.php?crop_id=MjA=&subject_id=MjM=&subject_matter_id=Mzc. Accesed on October 10, 2022.

Lakic Z, StankovicS, Pavlovic S, Krnjajic S and Popovic V. 2019. Genetic variability in quantitative traits of field pea (Pisum sativum L.) genotypes. Czech Journal of Genetics and Plant Breeding 55(1): 1–7.

Loridon K, Mc Phee K, Morin J, Dubreuil P, Pilet-Nayel M L, Aubert G, Ramea C, Baranger A, Coyne C, Lejeune-Henaut I and Burstin J. 2005. Microsatellite marker polymorphism and mapping in pea (Pisum sativum L.). Theoretical and Applied Genetics 111: 1022–31.

Macwilliam S, Wismer M and Kulshreshtha S. 2014. Life cycle and economic assessment of western Canadian pulse systems: The inclusion of pulses in crop rotations. Agricultural Systems 123: 43–53.

Munjal R L, Chenulu V V and Hora T S. 1963. Assessment of losses due to powdery mildew (Erysiphe polygoni) on pea. Indian Phytopathology 19: 260–67.

Patil B S, Bharadwaj C and Vijaykumar A G. 2023. Genetic enhancement of yield traits in chickpea (Cicer arietinum L.): An analysis of selection in early segregating generations. Legume Research 46(5): 579–83.

Reddy L D C, Preethi B, Wani M A, Aghora T S, Aswath C and Mohan N. 2015. Screening for powdery mildew (Erysiphe pisi D C) resistance gene linked SCAR and SSR markers in five breeding lines of Pisum sativum (L.). The Journal of Horticultural Science and Biotechnology 90(1): 78–82.

Saari E E and Prescott J M. 1975. A scale for appraising foliar intensity of wheat diseases. Plant Diseases Reporter 59: 377–80.

Saxena R R, Vidayakar V, Vishkarma M V, Yadav P S, Meena M L and Lal G M. 2014. Genetic variability and heritability analysis for some quantitative traits in field pea (Pisum sativum L.). The Bioscan 9(2): 895–98.

Shivakumar M S, Kalimath P M, Biradar S S, Timmanna P O and Shridevi O. 2013. Assesment of variability and identification of transgressive segregants for yield and yield component traits in early segregating generations of chickpea. Legume Genomics and Genetics 4(3): 22–26.

Singh S, Verma V, Singh B, Sharma V R and Kumar M. 2019. Genetic variability, heritability and genetic advance studies in pea (Pisum sativum L.) for quantitative characters. The Indian Journal of Agricultural Research 53: 542–47.

Srivastava R K, Mishra S K, Singh A K and Mohapatra T. 2012. Development of a coupling-phase SCAR marker linked to the powdery mildew resistance gene ‘er1’ in pea (Pisum sativum L.). Euphytica 186: 855–66.

Sunil J, Atul K, Sandeep K L, Jameel A, Muraleedhar A, Gyan P M and Shaily J. 2022. SCAR marker development for quick detection of Fusarium oxysporum f. sp. lentis. The Indian Journal of Agricultural Sciences 92(2): 278–80.

Sulima A S and Zhukov V A. 2022.War and peas: Molecular bases of resistance to powdery mildew in pea (Pisum sativum L.) and other legumes. Plants 11: 339.

Singh M, Rani S, Malhotra N, Katna G and Sarker A. 2018. Transgressive segregations for agronomic improvement using interspecific crosses between C. arietinum L. × C. reticulatum Ladiz. and C. arietinum L. × C. echinospermum Davis species. PLOS ONE 13(9): e0203082.

Sun S, Deng D, Wu W, He Y, Luo G, Du C, Duan C and Zhu Z. 2022. Molecular characterizations of the er1 alleles conferring resistance to Erysiphe pisi in three chinese pea (Pisum sativum L.) landraces. International Journal of Molecular Sciences 23(19): 12016.