Influence of spermidine priming on rice (Oryza sativa) seed germinability and vigour under heat stress

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  • H R ARCHANA ICAR-Indian Agricultural Research Institute, New Delhi 110 012, India
  • DUNNA VIJAY ICAR-Indian Agricultural Research Institute, New Delhi 110 012, India
  • MANJUNATH PRASAD C T ICAR-Indian Agricultural Research Institute, New Delhi 110 012, India
  • DILSHAD AHMAD ICAR-Indian Agricultural Research Institute, New Delhi 110 012, India
  • ARUN KUMAR M B ICAR-Indian Agricultural Research Institute, New Delhi 110 012, India
  • PROLAY KUMAR BHOWMICK ICAR-Indian Agricultural Research Institute, New Delhi 110 012, India
  • SUBODH KUMAR SINHA ICAR-National Institute of Plant Biotechnology, New Delhi
  • D K SHARMA ICAR-Indian Agricultural Research Institute, New Delhi 110 012, India
  • L C SUSHMITHA ICAR-Indian Agricultural Research Institute, New Delhi 110 012, India


α-Amylase activity, High-temperature stress, PEG, Seed Priming, Thermotolerance


Polyamines (PAs) play a vital role in plants' response to various abiotic stresses, including high temperature (HT) stress. The present study was carried out during 2020–23 at the Indian Agricultural Research Institute, New Delhi to explores how spermidine treatment affects rice (Oryza sativa L.) seeds' ability to withstand heat and the availability of sugars for seedling growth during the radicle stage in heat-susceptible (IR64) and tolerant (N22) varieties. The seeds were primed with 1 mM spermidine using PEG (-1 MPa) as the priming medium, followed by exposure to heat stress. Results showed that in unprimed seeds, heat stress significantly reduces the seed germination, vigour and sugar availability in both the varieties and to a greater extent in IR64 compared to N22. This difference resulted in a substantially lower percentage of normal seedlings and seed vigour index in IR64 than in N22. However, spermidine seed priming enhanced thermotolerance in both varieties, more in IR64, equating the normal seedling percentage and seedling growth with control, i.e. without heat stress. The priming treatment also notably augmented α-amylase activity and reducing sugar availability, particularly in N22, enabling better seedling growth under heat stress conditions. This study underscores the importance of seed priming with spermidine to allow the seedlings to tolerate elevated temperatures and maintain better seedling growth due to the enhanced availability of reducing sugars during germination and early seedling growth.


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Aktar N and Islam M R. 2017. Heat stress effects and management in wheat-A review. Agronomy for Sustainable Development 37: 37.

Azharudheen T M, Yadava D K, Singh N, Vasudev S and Prabhu K V. 2013. Screening Indian mustard [Brassica juncea (L.) Czern and Coss)] germplasm for seedling thermo-tolerance using a new screening protocol. African Journal of Agricultural Research 8(38): 4755–60.

Basra A S, Singh B and Malik C P. 1994. Priming-induced changes in polyamine levels in relation to vigor of aged onion seeds. Botanical Bulletin of Academia Sinica 35(1): 19–23.

Bewley J D, Bradford K, Hilhorst H and Nonogaki H. 2012. Seeds: Physiology of Development, Germination and Dormancy, 3rd edn. Springer, New York.

Chen Y S, David Ho T H, Liu L, Lee D H, Lee C H, Chen Y R, Lin S Y, Lu C A and Yu S M. 2019. Sugar starvation-regulated MYBS2 and 14-3-3 protein interactions enhance plant growth, stress tolerance, and grain weight in rice. Proceedings of the National Academy of Sciences 116(43): 21925–35.

Collado-Gonzalez J, Pinero M C, Otalora G, Lopez-Marin J and del Amor F M. 2020. Exogenous spermidine modifies nutritional and bioactive constituents of cauliflower (Brassica oleracea var. botrytis L.) florets under heat stress. Scientia Horticulturae 277: 109818.

Ella E S, Dionisio-Sese M L and Ismail A M. 2011. Seed pre- treatment in rice reduces damage, enhances carbohydrate mobilization and improves emergence and seedling establishment under flooded conditions. AoB Plants 2011: plr007.

Farooq M, Aziz T, Rehman H, Rehman A, Cheema S A and Aziz T. 2011. Evaluating surface drying and re-drying for wheat seed priming with polyamines: effects on emergence, early seedling growth and starch metabolism. Acta Physiologiae Plantarum 33: 1707–13.

Fu Y, Zhang Z, Liu J, Chen M, Pan R, Hu W and Hu J. 2020. Seed priming with spermidine and trehalose enhances chilling tolerance of rice via different mechanisms. Journal of Plant Growth Regulation 39: 669–79.

Hussain S, Khaliq A, Ali B, Hussain H A, Qadir T and Hussain S. 2019. Temperature extremes: Impact on rice growth and development. Plant Abiotic Stress Tolerance: Agronomic, Molecular and Biotechnological Approaches, pp. 153–71.

Hasanuzzaman M, Hakeem K R, Nahar K and Alharby H F (Eds). Springer Nature, Switzerland.

ISTA Rules. 2022. International Rules for Seed Testing. International Seed Testing Association, Zurich, Switzerland.

Jagadish S V, Muthurajan R, Oane R, Wheeler T R, Heuer S, Bennett J and Craufurd P Q. 2010. Physiological and proteomic approaches to address heat tolerance during anthesis in rice (Oryza sativa L.). Journal of Experimental Botany 61(1): 143–56.

Kaneko M, Itoh H, Ueguchi-Tanaka M, Ashikari M and Matsuoka M. 2002. The α-amylase induction in endosperm during rice seed germination is caused by gibberellin synthesized in epithelium. Plant Physiology 128(4): 1264–70.

Kilasi N L, Singh J, Vallejos C E, Ye C, Jagadish S K, Kusolwa P and Rathinasabapathi B. 2018. Heat stress tolerance in rice (Oryza sativa L.): Identification of quantitative trait loci and candidate genes for seedling growth under heat stress. Frontiers in Plant Science 9: 1578.

Li Z, Peng Y, Zhang X Q, Ma X, Huang L K and Yan Y H. 2014. Exogenous spermidine improves seed germination of white clover under water stress via involvement in starch metabolism, antioxidant defenses and relevant gene expression. Molecules 19(11): 18003–24.

Liu J H, Wang W, Wu H, Gong X and Moriguchi T. 2015. Polyamines function in stress tolerance: from synthesis to regulation. Frontiers in Plant Science 6: 827.

Michel B E and Kaufmann M R. 1973. The osmotic potential of polyethylene glycol 6000. Plant Physiology 51: 914–16.

Miller G L. 1972. Estimation of reducing sugar by dinitrosalicylic acid method. Analytical Chemistry 31: 426–28.

Paul S, Roychoudhury A, Banerjee A, Chaudhuri N and Ghosh P. 2017. Seed pre-treatment with spermidine alleviates oxidative damages to different extent in the salt (NaCl)-stressed seedlings of three indica rice cultivars with contrasting level of salt tolerance. Plant Gene 11: 112–23.

Poli Y, Basava R K, Panigrahy M, Vinukonda V P, Dokula N R, Voleti S R, Desiraju S and Neelamraju S. 2013. Characterization of a Nagina22 rice mutant for heat tolerance and mapping of yield traits. Rice 6: 1–9.

Salleh M S, Nordin M S and Puteh A B. 2020. Germination performance and biochemical changes under drought stress of primed rice seeds. Seed Science and Technology 48: 333–43.

Sari A. 2021. The effect of high temperature on α-amylase enzyme activity in the germination of several rice varieties (Oryza sativa L.). JERAMI Indonesian Journal of Crop Science 3(2): 50–54.

Sharma N, Sharma J R, Malik A, Sharma A, Kumar V, Yadav R and Kumar A. 2022. Effect of priming treatments on germination and seedling growth of artificially aged seed of guava (Psidium guajava). The Indian Journal of Agricultural Sciences 92(4): 516–20.

Sheteiwy M, Shen H, Xu J, Guan Y, Song W and Hu J. 2017. Seed polyamines metabolism induced by seed priming with spermidine and 5-aminolevulinic acid for chilling tolerance improvement in rice (Oryza sativa L.) seedlings. Environmental and Experimental Botany 137: 58–72.

Singh T. 2022. Assessment of critical period for weeding and yield loss in direct seeded rice (Oryza sativa). The Indian Journal of Agricultural Sciences 92(2): 212–16.

Tamindzic G, Ignjatov M, Miljakovic D, Cervenski J, Milosevic D, Nikolic Z and Vasiljevic S. 2023. Seed priming treatments to improve heat stress tolerance of garden pea (Pisum sativum L.). Agriculture 13(2): 439.

Tang S, Zhang H, Li L, Liu X, Chen L, Chen W and Ding Y. 2018. Exogenous spermidine enhances the photosynthetic and antioxidant capacity of rice under heat stress during early grain-filling period. Functional Plant Biology 45(9): 911–21.

Tyagi A, Ali S, Ramakrishna G, Singh A, Park S, Mahmoudi H and Bae H. 2023. Revisiting the role of polyamines in plant growth and abiotic stress resilience: mechanisms, crosstalk, and future perspectives. Journal of Plant Growth Regulation 42: 5074–98.

Xu Y, Chu C and Yao S. 2021. The impact of high-temperature stress on rice: Challenges and solutions. The Crop Journal 9(5): 963–76.

Zhou R, Kjaer K H, Rosenqvist E, Yu X, Wu Z and Ottosen C O. 2017. Physiological response to heat stress during seedling and anthesis stage in tomato genotypes differing in heat tolerance. Journal of Agronomy and Crop Science 203(1): 68–80.









How to Cite

ARCHANA, H. R. ., VIJAY, D. ., C T, M. P. ., AHMAD, D., M B, A. K., BHOWMICK, P. K., SINHA, S. K., SHARMA, D. K., & SUSHMITHA, L. C. (2023). Influence of spermidine priming on rice (Oryza sativa) seed germinability and vigour under heat stress. The Indian Journal of Agricultural Sciences, 93(12), 1284–1290.