Enhancing the performance of chilli (Capsicum annuum) through twin role of plant growth promotion and disease suppressionvia Bacillus subtilis-based bioformulation

POPY  BORA; BISHAL  SAIKIA; MEHJEBIN  RAHMAN; SHENAZ SULTANA  AHMED; RAJASHREE  CHETIA; NASEEMA  RAHMAN; BHARAT CH  NATH; WASIM HASSAN  RAJA

doi:10.56093/ijas.v94i1.142692

Authors

POPY BORA Assam Agricultural University, Jorhat, Assam 785 013, India
BISHAL SAIKIA Assam Agricultural University, Jorhat, Assam 785 013, India
MEHJEBIN RAHMAN Assam Rice Research Institute, Assam Agricultural University, Titabar, Jorhat, Assam
SHENAZ SULTANA AHMED Assam Agricultural University, Jorhat, Assam 785 013, India
RAJASHREE CHETIA Assam Agricultural University, Jorhat, Assam 785 013, India
NASEEMA RAHMAN Horticultural Research Station, Kahikuchi, Assam Agricultural University, Kamrup, Assam
BHARAT CH NATH Assam Agricultural University, Jorhat, Assam 785 013, India
WASIM HASSAN RAJA ICAR-Central Institute of Temperate Horticulture, Srinagar, Kashmir

https://doi.org/10.56093/ijas.v94i1.142692

Keywords:

B. subtilis, Bioformulation, Chilli, Induced defense enzymes, Plant growth promotion

Abstract

Considering the majority of studies confined in vitro evaluation of microbial bioagents against diseases, a study was carried out during 2018–21 at Assam Agricultural University, Jorhat, Assam, (in vivo and field studies) to assess bio-efficacy of B. subtilis (strain LB22)-based liquid bioformulation against bacterial wilt (Ralstonia solanacearum) and anthracnose (Colletotrichum gloeosporioides) diseases of chilli (Capsicum annuum L.). In vivo efficacy of B. subtilis formulation (inclusive treatment involving seed treatment, seedling dip, soil treatment and foliar spray) showed significant suppressiveness against both the diseases coupled with enhanced growth and yield attributes. Subsequently, the field evaluation of Bacillus subtilis formulation comparing standard chemicals also edged over the latter by 15.45% in terms of yield gains in 2-years of study. A significant reduction was found in disease severity during field evaluation of Bacillus subtilis formulation on account of induced resistance via upregulated synthesis of plant defense-enzymes (Polyphenol oxidase, peroxidase, phenylalanine ammonia-lyase, and β-1,3-glucanase) by 2.3–11.0 folds with their peaks mostly expressed within 24–72 h. These results put forward a conceptual framework for delivery mechanism of a microbial bioagent in a formulation mode, having the potential to be effective against a number of other diseases as well.

Downloads

Download data is not yet available.

References

Ashwini N and Srividya S. 2014. Potentiality of Bacillus subtilis as a biocontrol agent for management of anthracnose disease of chilli caused by Colletotrichum gloeosporioides OGC1.3 Biotechnology 4: 127–36.

Bal S, Sharangi A B, Upadhyay T K, Khan F, Pandey P, Siddiqui S, Saeed M, Lee H J and Yadav D K. 2022. Biomedical and antioxidant potentialities in chilli: Perspectives and way forward. Molecules 27: 6380.

Bora P and Bora L C. 2020. Disease management in horticulture crops through microbial interventions: An overview. The Indian Journal of Agricultural Sciences 90(8): 1389–96.

Bora P and Bora L C. 2021. Microbial antagonists and botanicals mediated disease management in tea, Camellia sinensis (L.) O. Kuntze: An overview. Crop Protection 148: 105711.

Bora P, Bora L C, Bhuyan R P, Hashem A and Abd-Allah E. 2022. Bioagent consortia assisted suppression in grey blight disease with enhanced leaf nutrients and biochemical properties of tea (Camellia sinensis). Biological Control 170: 104907

Chester K S. 1959. How sick is the plant? Plant Pathology, Vol. 1, pp. 199–242. Horsfall J G and Diamond A E (Eds). Academic Press, New York, USA.

Choudhary J S, Monobrullah M D, Kumar R, Raghav D K and Singh A K. 2022. Field efficacy of insecticides against chilli thrips (Scirtothrips dorsalis) and their effect on coccinellids. The Indian Journal of Agricultural Sciences 92(10): 1196–1201.

Feng F, Chen X, Wang Q, Xu W, Long L, El-Masry G N and Yu X. 2020. Use of Bacillus-siamensis-inoculated biochar to decrease uptake of dibutyl phthalate in leafy vegetables. Journal of Environmental Management 253: 109636.

Ferrusquia-Jimenez N I, Gonzalez-Arias B, Rosales A, Eaquivel K, Escamilla-Silvia E M, Ortega-Torres A E and Guevara-Gonzalez

R G. 2022. Elicitation of Bacillus cereus-Amazcala (B.c-A) with SiO2 nanoparticles improves its role as growth promoting bacteria (PGPB) in chilli pepper plants. Plants 11(24): 3445. doi.org 110.3390/plants 11243445

Gowtham H G, Murali M, singh S B, Lakshmeesha T P, Marasimha Murthy k, Amruthest K N and Niranjana S R. 2018. Plant growth promoting rhizobacteria Bacillus amyloliquefaciens improves plant growth and reduces resistance in chilli against anthracnose disease. Biological Control 126: 209–12.

Hyder S, gondal A S, Rizvi Z F, Sajjad Haider M I and Inam- ul-Haq M. 2020. Biological control of chilli damping off disease caused by Pythium myriotylum. BioRxiv. doi. org/10.1101/2020.07.28.224519

Jena P K, Nayak B K, Rout M and Sahu K C. 2021. Anthracnose of chilli caused by Colletotrichum capsci and its management. International Journal of Research and Analytical Reviews 8(4): 815–18.

Jinal N H and Amaresan N. 2020. Evaluation of biocontrol Bacillus species on plant growth promotion and systemic-induced resistant potential against bacterial and fungal wilt-causing pathogens. Archive of Microbiology. doi:10.1007/s00203- 020-01891-2

Jyapala N, Mallikarjunaiah N H, Puttaswamy H, Gavirangappa H and Ramachandrappa H S. 2019. Rhizobacteria Bacillus spp. induce resistance against anthracnose disease of chilli (Capsicum annuum L.) through activating host defense response. Egyptian Journal of Pest Control 29(1): 1–9. doi. org/101186/s41938-019-0148-2

Kashyap A S, Manzar N, Rajawat M V S, Kesharwani A K, Singh R P, Dubey S C and Singh D. 2021. Screening and biocontrol potential of rhizobacteria native to Gangetic plains and hilly regions to induce systemic resistance and promote plant growth in chilli against bacterial wilt disease. Plants 10(10): 2125.

Kumar A, Rabha J and Jha D K. 2021. Antagonistic activity of lipopeptide-biosurfactant producing Bacillus subtilis AKP, against Colletotrichum capsici, the causal organism of anthracnose disease of chilli. Biocatalysis and Agricultural Biotechnology 36: 102133.

Mayer A M, Harel E and Shaul R B. 1965. Assay of catechol oxidase: A critical comparison of methods. Phytochemistry 5: 783–89.

Mckinney H H. 1923. A new system of grading plant diseases. Journal of Agricultural Research 26: 195–218.

Miljakovic D, Marinkovic J and Balesevic-Tubic S. 2020. The Significance of Bacillus spp. in disease suppression and growth promotion of field and vegetable crops. Microorganisms 8: 1037.

Natarajan J, Christobell C, Kumar D M, Balakumaran M D, Kumar M R and Kalaichelvan P T. 2012. Isolation and characterization of Ŝ-galactosidase producing Bacillus sp. from dairy effluent. World Applied Sciences Journal 17(11): 1466–74.

Pan S Q, Ye X S and Kuc J. 1991. Association of β-1, 3-glucanase activity and isoform pattern with systemic resistance to blue mould in tobacco induced by stem injection with Peronospora tabacina or leaf inoculation with tobacco mosaic virus. Physiological and Molecular Plant Pathology 39(1): 25–39.

Prihatiningsih N, Djatmiko H A and Erminawati W. 2019. Bio-management of anthracnose disease in chilli with microencapsulates containing Bacillus subtilis B298. Earth and Environmental Sciences 250: 012041.

Rahman M, Borah S M, Borah P K, Bora P, Sarmah B K, Lal M K and Kumar R. 2023. Deciphering the antimicrobial activity of multifaceted rhizospheric biocontrol agents of solanaceous crops, viz. Trichoderma harzianum MC2, and Trichoderma harzianum NBG. Frontiers in Plant Science 14: 1141506.

Ramzan N, Noreen N, Perveen Z and Shahzad S. 2016. Effect of seed pelleting with biocontrol agents on growth and colonization of roots of mungbean by root-infecting fungi. Journal of the Science of Food and Agriculture 96: 3694–3700.

Ross W W and Sederoff R R. 1992. Phenylalanine ammonia-lyase from loblolly Pine: Purification of the enzyme and isolation of complementary DNA clone. Plant Physiology 98: 380386.

Saikia S, Bora P and Bora L C. 2021. Bioagent mediated management of citrus canker. The Indian Journal of Agricultural Sciences 91: 198–201.

Saikia B, Bora P, Taye T, Chetia R, Tabing R, Neog T and Nayak S. 2023. Biocontrol potential of Bacillus subtilis Lb22 against fruit rot of King chilli, Capsicum chinense Jacq. Pest Management in Horticultural Ecosystems 28: 173–79.

Saxena A, Raghuwanshi R, Gupta V K and Singh H B. 2016. Cilli anthracnose; The eidemiology and management. Frontiers in Microbiology 7. doi.org/10.3389/fmicb2016.01527.

Smith J A and Hammerschmidt R. 1988. Comparative study of acidic peroxidases associated with induced resistance in cucumber, muskmelon, and watermelon. Physiological and Molecular Plant Pathology 33(2): 255–61.

Srivastava A K, Das A K, Jagannadham P T K, Bora P, Ansari F A and Bhate R. 2022. Bioprospecting microbiome for soil and plant health management amidst huang longbing threat in citrus: A review. Frontiers in Plant Science 13: 858842.

Suma M, Singh N, Buttar D S and Hunjan M S. 2023. Management of damping off disease in tomato (Solanum lycopersicum) using potential biocontrol agent Pseudomonas fluorescens. The Indian Journal of Agricultural Sciences 93(5): 549–54. https://doi.org/10.56093/ijas.v93i5.132109

Wang T, Wang X, Han M, Song X, Yang D, Wang S, Laborda P and Shi X. 2021. Enhanced spoVF operon increases host attachment and biocontrol ability of Bacillus subtilis for the management of Ceratocystis fimbriata in sweet potato. Biological Control 161: 104651.

Winstead N N and Kelman A. 1952. Inoculation techniques for evaluating resistance to Pseudomonas solanacearum. Phytopathology 42: 628–34.

Xie D, Cai X, Yang C, Xie L, Qin G, Zhang M, Huang Y, Gong G, Chang X and Chen H. 2021. Studies on the control effect of Bacillus subtilis on wheat powdery mildew. Pest Management Science 77(10): 4375–82.

Yang N, Yang Q, Chen J and Fisk I. 2021. Impact of capsaicin on aroma release and perception from flavoured solutions. LWT 138: 110613.

Yuan S, Wu Y, Jin J, Tong S, Zhang L and Cai Y. 2023. Biocontrol capabilities of Bacillus subtilis E11 against Aspergillus flavus in vitro and for dried red chili (Capsicum annuum L.). Toxins 15: 308.