Unraveling the influence of salinity on physiological and biochemical parameters in citrus (Citrus spp.) rootstocks


Abstract views: 75 / PDF downloads: 136

Authors

  • REETIKA Chaudhary Charan Singh Haryana Agricultural University, Hisar, Haryana 125 004, India
  • R P S DALAL CCS Haryana Agricultural University, Hisar, Haryana
  • SOURABH ICAR-Central Arid Zone Research Institute, Jodhpur, Rajasthan
  • RUPAKSHI Chaudhary Charan Singh Haryana Agricultural University, Hisar, Haryana 125 004, India
  • VIVEK BENIWAL Chaudhary Charan Singh Haryana Agricultural University, Hisar, Haryana 125 004, India
  • TANVI MEHTA Chaudhary Charan Singh Haryana Agricultural University, Hisar, Haryana 125 004, India
  • RAVI GAUTAM Chaudhary Charan Singh Haryana Agricultural University, Hisar, Haryana 125 004, India

https://doi.org/10.56093/ijas.v94i2.144766

Keywords:

Chlorophyll, Citrus, MDA, Rootstock, RSI, RWC, Salinity

Abstract

The present study was carried out during 2018–19 and 2019–20 at Chaudhary Charan Singh Haryana Agricultural University, Hisar, Haryana to examine the impact of salt stress on physiological parameters of 9 distinct rootstocks of citrus (Citrus spp.) The experimental design followed a completely randomized design (CRD) accompanied with 3 replications, enclosing 45 combinations with 9 citrus rootstocks (Pectinifera, Cleopatra mandarin, Rangpur lime, Alemow, Rough lemon, NRCC-4, Volkamer lemon, CRH-12 and NRCC-3) exposed to 5 NaCl salt stress levels, viz. control (0.07), 2.5, 4.0, 5.5 and 7.0 dS/m. Among the different rootstocks, Rangpur lime exhibited the highest leaf (12.65 mg/g DW) and root (12.42 mg/g DW) total soluble carbohydrates at the 7.0 dS/m salinity level. Additionally, Rangpur lime showcased minimal reduction in chlorophyll stability index (17.2%), leaf and root relative water content (18.7 and 18.9%, respectively), relative stress injury (32.0 and 33.0%, respectively) and leaf and root (Malondialdehyde) MDA content (8.46 and 8.12 µmoles/g DW, respectively) at the same salinity level. Overall, Rangpur lime, Volkamer lemon and CRH-12 demonstrated superior performance by exhibiting relatively higher buildup of total soluble carbohydrates and less drop in CSI, RWC, RSI and MDA content at 7.0 dS/m as compared to control. Conversely, Cleopatra mandarin, Rough lemon and NRCC-3 displayed a relative moderate response, while Pectinifera, Alemow, and NRCC-4 showcased substandard performance, exhibiting contrasting behaviour at 7.0 dS/m as compared to control, particularly concerning physiological parameters at the seedling stage.

Downloads

Download data is not yet available.

References

Abadi F S G, Mostafavi M, Eboutalebi A, Samavat S and Ebadi A. 2010. Biomass accumulation and proline content of six citrus rootstocks as influenced by long-term salinity. Research Journal of Environmental Sciences 4(2): 158–65.

Alam A, Ullah H, Attia A and Datta A. 2020. Effects of salinity stress on growth, mineral nutrient accumulation and biochemical parameters of seedlings of three citrus rootstocks. International Journal of Fruit Science 20(4): 786–804.

Anonymous. 2023. Ministry of Agriculture and Farmer’s Welfare, New Delhi. https://agricoop.nic.in/en/StatHortEst

Ashraf M and Harris P J C. 2013. Photosynthesis under stressful environments. An overview. Photosynthetica 51(2): 163–90.

Barrs H D and Weatherley P E. 1962. A re-examination of the relative turgidity technique for estimating water deficit in leaves. Australian Journal of Biological Sciences 15: 413–28.

Dubois M, Gilíes K A, Hamilton J K, Rebers P A and Smith F. 1951. A colorimetric method for the determination of sugars. Nature 167–68.

Hassine A B and Lutts S. 2010. Differential responses of saltbush Atriplex halimus (L.) exposed to salinity and water stress in relation to senescing hormones abscisic acid and ethylene. Journal of Plant Physiology 167(17): 1448–456.

Heath R L and Packer L. 1968. Photoperoxidation in isolated chloroplasts: I. Kinetics and stoichiometry of fatty acid peroxidation. Archives of Biochemistry and Biophysics 125(1): 189–98.

Hiscox J D and Isrealstam G F. 1979. A method for the extraction of chlorophyll from leaf tissue without maceration. Canadian Journal of Botany 57: 1332–34.

Hussain K, Majeed A, Nawaz K and Nisar M F. 2009. Effect of different levels of salinity on growth and ion contents of black seeds (Nigella sativa L.). Current Research Journal of Biological Sciences 1(3): 135–38.

Khoshbakht D, Ramin A A and Baninasab B. 2014. Citrus rootstocks response to salinity: Physio-biochemical parameters changes. Research Journal of Environmental Sciences 8(1): 29–38.

Ma Y, Dias M C and Freitas H. 2020. Drought and Salinity Stress Responses and microbe-induced tolerance in plants. Frontiers in Plant Science 11: 591911.

Montoliu A, Lopez-Climent M F, Arbona V, Perez-Clemente R M and Gomez-Cadenas A. 2009. A novel in vitro tissue culture approach to study salt stress responses in citrus. Plant Growth Regulation 59(2): 179–87.

Murkute A A, Sharma S and Singh S K. 2010. Biochemical alterations in foliar tissues of citrus genotypes screened in vitro for salinity tolerance. Journal of Plant Biochemistry and Biotechnology 19(2): 203–08.

Pérez-Jiménez M and Perez-Tornero O. 2020. Improved salt- tolerance in Citrus macrophylla mutant rootstocks. Scientia Horticulturae 259: 108815.

Pérez-Tornero O, Tallon C I, Porras I and Navarro J M. 2009. Physiological and growth changes in micro propagated Citrus macrophylla explants due to salinity. Journal of Plant Physiology 166(17): 1923–33.

Ruiz M, Pensabene-Bellavia G, Quinones A, García-Lor A, Morillon R, Ollitrault P, Primo-Millo E, Navarro L and Aleza P. 2018. Molecular characterization and stress tolerance evaluation of new allotetraploid somatic hybrids between Carrizo citrange and Citrus macrophylla W. rootstocks. Frontiers in Plant Science 9: 901.

Şahin-Çevik M, Çevik B and Coşkan A. 2020. Identification and expression analysis of salinity-induced genes in rangpur lime (Citrus limonia). Horticultural Plant Journal 6(5): 267–76.

Sairam R K, Deshmukh P S and Saxena D C. 1997. Role of antioxidant systems in wheat genotypes tolerance to water stress. Biologia Plantarum 41: 387–94.

Shahid M A, Balal R M, Khan N, Simon-Grao S, Alfosea-Simon M, Camara-Zapata J M, Mattson N S and Garcia-Sanchez F. 2019. Rootstocks influence the salt tolerance of Kinnow mandarin trees by altering the antioxidant defense system, osmolyte concentration, and toxic ion accumulation. Scientia Horticulturae 250: 1–11.

Sharma L K, Kaushal M, Bali S K and Choudhary O P. 2013. Evaluation of rough lemon (Citrus jambhiri Lush.) as rootstock for salinity tolerance at seedling stage under in vitro conditions. African Journal of Biotechnology 12(44): 6267–75.

Sheoran O P, Tonk D S, Kaushik L S, Hasija R C and Pannu R S. 1998. Statistical software package for agricultural research workers. Department of Mathematics Statistics, CCS Haryana Agricultural University, Hisar, Haryana. pp. 139–43.

Simpson C R, Nelson S D, Melgar J C, Jifon J, Schuster G and Volder A. 2015. Effects of salinity on physiological parameters of grafted and ungrafted citrus trees. Scientia Horticulturae 197: 483–89.

Stover E, Hall D G, Grosser J, Gruber B and Moore G A. 2018. Huanglongbing-related responses of ‘Valencia’ Sweet Orange on eight citrus rootstocks during greenhouse trials. Hort Technology 28(6): 776–82.

Sullivan C Y and Ross W M. 1979. Selecting for drought and heat resistance in grain sorghum. Stress Physiology in Crop Plant, pp. 263–81.

R C John Wiley and Sons Ltd. Publisher, New York. Xue X, Liu A and Hua X. 2009. Proline accumulation and transcriptional regulation of proline biosynthesis and degradation in Brassica napus. BMB Reports 42(1): 28–34.

Zou Z, Xi W, Hu Y, Nie C and Zhou Z. 2016. Antioxidant activity of citrus fruits. Food chemistry 196: 885–96.

Downloads

Submitted

2023-11-02

Published

2024-02-13

Issue

Section

Articles

How to Cite

REETIKA, DALAL, R. P. S., SOURABH, RUPAKSHI, BENIWAL, V., MEHTA, T., & GAUTAM, R. . (2024). Unraveling the influence of salinity on physiological and biochemical parameters in citrus (Citrus spp.) rootstocks. The Indian Journal of Agricultural Sciences, 94(2), 167–173. https://doi.org/10.56093/ijas.v94i2.144766
Citation