GC-MS analysis of metabolites in tritrophic interaction of yeast (Pichia Kudriavzevii)against soft rot pathogen of carrot (Daucus carota)


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Authors

  • S LAVANYA Tamil Nadu Agricultural University, Coimbatore, Tamil Nadu 641 003, India image/svg+xml
  • P MUTHULAKSHMI Tamil Nadu Agricultural University, Coimbatore, Tamil Nadu 641 003, India image/svg+xml
  • I JOHNSON Tamil Nadu Agricultural University, Coimbatore, Tamil Nadu 641 003, India image/svg+xml

https://doi.org/10.56093/ijas.v95i1.145394

Keywords:

Carrot, GC-MS, Metabolite, Post-harvest pathogens, Yeast

Abstract

Carrot a fleshy edible root vegetable, abundant in bioactive compounds such as carotenoids and dietary fibres. The market value of carrots is impacted by post-harvest illnesses, which have emerged as a serious production constraint. A promising advancement in the battle against post-harvest illnes is yeast-based biocontrol, because of its colonization on dry surfaces for longer periods and low nutrient utilization and can multiply easily. A study was carried out during 2022–23 at Tamil Nadu Agricultural University, Coimbatore, Tamil Nadu to evaluate the GC- MS analysis of metabolites in tritrophic interaction of yeast (Pichia Kudriavzevii) against soft rot pathogen of carrot. The tritrophic interaction of soft rot pathogen Pectobacterium carotovorum subsp. carotovorum and yeast isolate YKP 12 Pichia kudriavzevii was analyzed through GCMS analysis and the metabolite 1-Hexanol, 2-ethyl showed highest peak area of 13.89% with the retention time of 4.89 min. The tri-trophic interaction results revealed that the compound 1, 2-Benzenedicarboxylic acid monoester had both antibacterial and antifungal activity.

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References

Chiranjeevi N, Reddi Kumar M, Padmodaya B, Venkateswarlu N C, Sudhakar P, Sarada Jayalakshmi Devi R and Aruna Sri P. 2021. Studies on extraction, evaluation of crude metabolite extract from endophytic Bacillus subtillis and its mechanistic effect on chickpea dry root rot causing pathogen Rhizoctonia bataticola (Taub.) Butler. Pharma Innovation 10: 898–905.

Gomez K A and Gomez A A. 1984. Statistical Procedures for Agricultural Research, 2nd edn, pp. 680. John Wiley and Sons, New York.

Hewavitharana S S, Ruddell D and Mazzola M. 2014. Carbon source-dependent antifungal and nematicidal volatiles derived during anaerobic soil disinfestation. European Journal of Plant Pathology 140: 39–52. DOI: https://doi.org/10.1007/s10658-014-0442-5

Joshi S, Amatya S, Pandey R D, Khadka P and Bhattarai J. 2020. Antimicrobial, antioxidant, antidiabetic, cytotoxic activities and GC-MS analysis of methanolic extract of Elaeocarpus sphaericus leaves from Nepal. International Journal of Advanced Research in Chemical Science 8(1): 11–23.

Kawahara H, Hirai F and Obata H. 1996. Antibacterial effect of ω-Cyclopentane fatty acids synthesized by Bacillus subtilis 626 from cyclopentane carboxylic acid. Biocontrol Science 1(1): 69–72. DOI: https://doi.org/10.4265/bio.1.69

Kitinoja L, Tokala V Y and Brondy A. 2018. Challenges and opportunities for improved postharvest loss measurements in plant-based food crops. Journal of Postharvest Technology 6(4): 16–34.

Liu X, Gao Y, Yang H, Li L, Jiang Y, Li Y and Zheng J. 2020. Pichia kudriavzevii retards fungal decay by influencing the fungal community succession during cherry tomato fruit storage. Food Microbiology 88: 103404. DOI: https://doi.org/10.1016/j.fm.2019.103404

Ma J, Godana E A, Yang Q and Zhang H. 2023. Effect of the antagonistic yeast Hannaella sinensis on the degradation of Patulin. Biological Control 178: 105134. DOI: https://doi.org/10.1016/j.biocontrol.2022.105134

Manimaran M and Kannabiran K. 2017. Marine Sp. VITMK1 derived Pyrrolo [1, 2-A] Pyrazine-1, 4-Dione, Hexahydro-3- (2-Methylpropyl) and its free radical scavenging activity. The Open Bioactive Compounds Journal 5(1). DOI: https://doi.org/10.2174/1874847301705010023

Marquez-Villavicencio M D P, Weber B, Witherell R A, Willis D K and Charkowski A O. 2011. The 3-hydroxy-2-butanone pathway is required for Pectobacterium carotovorum pathogenesis. PloS One 6(8): e22974.1. DOI: https://doi.org/10.1371/journal.pone.0022974

Marrufo T, Nazzaro F, Mancini E, Fratianni F, Coppola R, De Martino L and De Feo V. 2013. Chemical composition and biological activity of the essential oil from leaves of Moringa oleifera Lam. cultivated in Mozambique. Molecules 18(9): 10989–11000. DOI: https://doi.org/10.3390/molecules180910989

Meziani S, Oomah B D, Zaidi F, Simon-Levert A, Bertrand C and Zaidi Yahiaoui R. 2015. Antibacterial activity of carob (Ceratonia siliqua L.) extracts against phytopathogenic bacteria Pectobacterium atrosepticum. Microbial Pathogenesis 78: 95–102. DOI: https://doi.org/10.1016/j.micpath.2014.12.001

Momin K and Thomas S C. 2020. GC-MS analysis of antioxidant compounds present in different extracts of an endemic plant Dillenia scabrella (Dilleniaceae) leaves and barks. International Journal of Pharmaceutical Sciences and Research 11: 2262–73.

Mulatu A, Megersa N, Tolcha T, Alemu T and Vetukuri R R. 2022. Antifungal compounds, GC-MS analysis and toxicity assessment of methanolic extracts of Trichoderma species in an animal model. PloS One 17(9): e0274062. DOI: https://doi.org/10.1371/journal.pone.0274062

Sasidharan K, Soga T, Tomita M and Murray D B. 2012. A yeast metabolite extraction protocol optimised for time-series analyses. e44283. DOI: https://doi.org/10.1371/journal.pone.0044283

Sheeba H, Ali M S and Anuradha V. 2019. Bioactive compounds and antimicrobial activity of fungal crude extract from medicinal plants. Journal of Pharmaceutical Sciences and Research 11(5): 1826–33.

Spadaro D and Droby S. 2016. Development of biocontrol products for postharvest diseases of fruit: The importance of elucidating the mechanisms of action of yeast antagonists. Trends in Food Science and Technology 47: 39–49. DOI: https://doi.org/10.1016/j.tifs.2015.11.003

Yogeswari S, Ramalakshmi S, Neelavathy R and Muthumary J Y. 2012. Identification and comparative studies of different volatile fractions from Monochaetia kansensis by GCMS. Global Journal of Pharmacology 6(2): 65–71.

Zhang X, Li B, Zhang Z, Chen Y and Tian S. 2020. Antagonistic yeasts: A promising alternative to chemical fungicides for controlling postharvest decay of fruit. Journal of Fungi 6(3): 158. DOI: https://doi.org/10.3390/jof6030158

Zhao Q, Shi Y, Ngea G L N, Zhang X, Yang Q, Zhang Q and Zhang H. 2023. Changes of the microbial community in kiwifruit during storage after postharvest application of Wickerhamomyces anomalus. Food Chemistry 404: 134593. DOI: https://doi.org/10.1016/j.foodchem.2022.134593

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Submitted

2023-11-17

Published

2025-02-04

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Section

Short-Communication

How to Cite

LAVANYA, S. ., MUTHULAKSHMI, P. ., & JOHNSON, I. . (2025). GC-MS analysis of metabolites in tritrophic interaction of yeast (Pichia Kudriavzevii)against soft rot pathogen of carrot (Daucus carota). The Indian Journal of Agricultural Sciences, 95(1), 105–108. https://doi.org/10.56093/ijas.v95i1.145394
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