Decomposed chicken feather: A biostimulant to lettuce (Lactuca sativa) growth

LEILI  AHMADI; JILA  BAHARLOUEI; KAZEM  KHAVAZI; MOHAMMAD HOSSEIN  DAVOUDI

doi:10.56093/ijas.v94i3.145669

Authors

LEILI AHMADI Islamic Azad University, Isfahan (Khorasgan Branch), Iran
JILA BAHARLOUEI Islamic Azad University, Isfahan (Khorasgan Branch), Iran
KAZEM KHAVAZI Soil and Water Research Institute, Agricultural Research, Education and Extension Organization, Karaj, Iran
MOHAMMAD HOSSEIN DAVOUDI Soil and Water Research Institute, Agricultural Research, Education and Extension Organization, Karaj, Iran

https://doi.org/10.56093/ijas.v94i3.145669

Keywords:

Biodegradation, Growth regulators biostimulants, Soil bacteria, Waste

Abstract

It is crucial to produce plant growth biostimulants from waste and renewable resources. A study was carried during 2020 at the Soil and Water Research Institute, Karaj, Iran to evaluate the effect of decomposed chicken feather as a biostimulant on lettuce (Lactuca sativa L.) growth. A total of 29 soil samples from 15 Iranian regions were used to isolate and identify the bacteria and 31 strains that were able to produce keratinase and capable of feather degrading were isolated. Then, 8 isolates that were able to degrade the feather during 7 days were selected as the superior strains. Based on the results Bacillus siamensis c11, Bacillus methylotrophicus gh1 and Bacillus methylotrophicus a2 were identified as the new strains that can produce keratinase enzyme. Then, the effect of foliar spraying of the solutions produced from feather degradation by the new strains (c11, gh1, and a2) on the growth of lettuce was investigated. The results showed that the solution produced by methylotrophicus gh1 strain significantly increased the fresh and dry weight of the shoot and root (respectively, 25.9, 36.9, 34.1 and 51.9% increase compared to the control). It is concluded that the microbial solution of these three microbes as a consortium could be quite a new addition on one hand, could also reduce the human allergy as a via- medium of waste disposal.

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References

Abdel-fattah A M, Nashy E-S H, Sabiel E-T A, Hussien M M and Attia A S. 2015. Novel keratinolytic activity of cyberlindnera fabianii nrc3 aza as a plant growth promoting agent (PGPA). International Journal of Applied Sciences and Biotechnology 3(4): 609–18. DOI: https://doi.org/10.3126/ijasbt.v3i4.13683

Almahasheer A, Mahmoud A, El-Komy H, Alqosaibi A and Aktar S. 2022. Novel feather degrading keratinases from bacillus cereus group: Biochemical, genetic and bioinformatics analysis. Microorganisms 10(1): 93. doi: 10.3390/microorganisms10010093 DOI: https://doi.org/10.3390/microorganisms10010093

Bach E, Cannavan F S, Duarte F R, Taffarel J A, Tsai S M and Brandelli A. 2011. Characterization of feather-degrading bacteria from Brazilian soils. International Biodeterioration and Biodegradation 65(1): 102–07. DOI: https://doi.org/10.1016/j.ibiod.2010.07.005

Basirat M, Mousavi S M, Dehghani F and Davoudi M H. 2023. Exploratory research on the adoption of new organic wastes for production of greenhouse cucumber in soilless culture. Waste and Biomass Valorization 14(7): 2367–374. DOI: https://doi.org/10.1007/s12649-022-01995-4

Bumbra P, Khosla B and Behl R K. 2022. Valorization of poultry feathers into value added products. Annals of Biology 38(1): 118–22.

Bhari R, Kaur M and Sarup Singh R. 2021. Chicken feather waste hydrolysate as a superior biofertilizer in agroindustry. Current Microbiology 78(6): 2212–230. DOI: https://doi.org/10.1007/s00284-021-02491-z

Bremner J. 1982. Total nitrogen. Methods of Soil Analysis, pp. 595–624. DOI: https://doi.org/10.2134/agronmonogr9.2.2ed.c31

Bremner J M. 1996. N-total. Methods of Soil Analysis 3: 1085–1121.

Callegaro K, Brandelli A and Daroit D J. 2019. Beyond plucking: Feathers bioprocessing into valuable protein hydrolysates. Waste DOI: https://doi.org/10.1016/j.wasman.2019.06.040

Management 95: 399–415.

Cheraghi M, Motesharezadeh B, Alikhani H A and Mousavi S M. 2022. Optimal management of plant nutrition in tomato (Lycopersicon esculentum Mill) by using biologic, organic and inorganic fertilizers. Journal of Plant Nutrition 1–20. DOI: https://doi.org/10.1080/01904167.2022.2092511

Emami A. 1996. Plant Decomposition Method 982: 8–90. Soil and Water Research Institute Publication.

Gee G W and Bauder J W. 1986. Particle-size analysis. Methods of Soil Analysis, Part 1: Physical and Mineralogical Methods 5: 383–411. DOI: https://doi.org/10.2136/sssabookser5.1.2ed.c15

Klute A. 1986. Methods of Soil Analysis, Part I: Physical and Mineralogical Methods. Soil Science Society of America, Inc. Publisher, Madison, WI. DOI: https://doi.org/10.2136/sssabookser5.1.2ed

Kshetri P, Roy S S, Sharma S K, Singh T S, Ansari M A, Prakash N and Ngachan S. 2019. Transforming chicken feather waste into feather protein hydrolysate using a newly isolated multifaceted keratinolytic bacterium Chryseobacterium sediminis RCM- SSR-7. Waste and Biomass Valorization 10: 1–11. DOI: https://doi.org/10.1007/s12649-017-0037-4

Li Q. 2019. Progress in microbial degradation of feather waste. Frontiers in Microbiology 10: 2717. https://doi.org/10.3389/fmicb.2019.02717 DOI: https://doi.org/10.3389/fmicb.2019.02717

Liaqat I, Ali S, Butt A, Durrani A I, Zafar U, Saleem S, Naseem S and Ahsan F. 2022. Purification and characterization of keratinase from bacillus licheniformis dcs1 for poultry waste processing. Journal of Oleo Science 71(5): 693–700. DOI: https://doi.org/10.5650/jos.ess21426

Loeppert R H and Suarez D L. 1996. Carbonate and gypsum. Methods of Soil Analysis, Part 3-Chemical Methods, pp. 437–74. DOI: https://doi.org/10.2136/sssabookser5.3.c15

Sparks D L, Page A L, Sumner M E, Tabatabai M A and Helmke P A (Eds). Soil Science Society of America Inc., Madison, WI, USA.

Moshiri F, Ebrahimi H, Ardakani M R, Rejali F and Mousavi S M. 2019. Biogeochemical distribution of Pb and Zn forms in two calcareous soils affected by mycorrhizal symbiosis and alfalfa rhizosphere. Ecotoxicology and Environmental Safety 179: 241–48. DOI: https://doi.org/10.1016/j.ecoenv.2019.04.055

Mousavi S M, Motesharezadeh B, Hosseini H M, Alikhani H and Zolfaghari A A. 2018a. Geochemical fractions and phytoavailability of zinc in a contaminated calcareous soil affected by biotic and abiotic amendments. Environmental Geochemistry and Health 40 (4): 1221–35. DOI: https://doi.org/10.1007/s10653-017-0038-z

Mousavi S M, Motesharezadeh B, Hosseini H M, Alikhani H and Zolfaghari A A. 2018b. Root-induced changes of Zn and Pb dynamics in the rhizosphere of sunflower with different plant growth promoting treatments in a heavily contaminated soil. Ecotoxicology and Environmental Safety 147: 206–16. https://doi.org/10.1016/j.ecoenv.2017.08.045 DOI: https://doi.org/10.1016/j.ecoenv.2017.08.045

Mousavi S M, Srivastava A and Cheraghi M. 2022. Soil health and crop response of biochar: An updated analysis. Archives of Agronomy and Soil Science 1–26. DOI: https://doi.org/10.1080/03650340.2022.2054998

Olsen S R 1954. Estimation of available phosphorus in soils by extraction with sodium bicarbonate. US Department of Agriculture.

Prajapati S, Koirala S and Anal A K. 2021. Bioutilization of chicken feather waste by newly isolated keratinolytic bacteria and conversion into protein hydrolysates with improved functionalities. Applied Biochemistry and Biotechnology 193: 2497–515. DOI: https://doi.org/10.1007/s12010-021-03554-4

Radkowski A. 2018. Influence of foliar fertilization with amino acid preparations on morphological traits and seed yield of timothy. Plant, Soil and Environment 64(5): 209–13. DOI: https://doi.org/10.17221/112/2018-PSE

Sheikhy J, Ronaghi A, Karimian N, Zarei M and Mousavi S. 2018. Effect of vermicompost and Rhizophagus irregularis fungi on yield, nutrient uptake and chlorophyll content of two wheat cultivars. Journal of Sol Biology 5(2): 149–61.

Shen N, Yang M, Xie C, Pan J, Pang K, Zhang H, Wang Y and Jiang M. 2022. Isolation and identification of a feather degrading Bacillus tropicus strain Gxun-17 from marine environment and its enzyme characteristics. BMC Biotechnology 22(1): 11. DOI: https://doi.org/10.1186/s12896-022-00742-w

Srivastava A, Sharma A and Suneetha V. 2011. Pelagia research library. European Journal of Experimental Biology 1(2): 56–63.

Srivastava A, Wu Q-S, Mousavi S M and Hota D. 2021. Integrated soil fertility management in fruit crops: An overview. International Journal of Fruit Science 21(1): 413–39. DOI: https://doi.org/10.1080/15538362.2021.1895034

Sun Z, Li X, Liu K, Chi X and Liu L. 2021. Optimization for production of a plant growth promoting agent from the degradation of chicken feather using keratinase producing novel isolate Bacillus pumilus JYL. Waste and Biomass Valorization 12: 1943–54. DOI: https://doi.org/10.1007/s12649-020-01138-7

Walkley A and Black A. 1934. An examination of the degtjareff method for determining soil organic matter, and a proposed modification of the chromic acid titration method. Soil Science 37(1): 29–38. DOI: https://doi.org/10.1097/00010694-193401000-00003