Isolation of Entomopathogenic fungus, Aspergillus flavus and its efficacy against Phenacoccus solenopsis (Pseudococcidae; Hemiptera)
12
Authors
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Dr. Ramyaa Lakshmi T S
Assistant Professor
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Ramya Vaideki G
Research scholar
Abstract
Phenacoccus solenopsis commonly known as cotton mealy bug is a harmful pest that affects cotton plants as well as ornamental plants and other agricultural products due to its ability to feed on a wide variety of plants. In an effort to find alternative solutions to synthetic insecticides, this study investigated the potential of the entomopathogenic fungus Aspergillus flavus as a control method for the 3rd nymphal instar of P. solenopsis in the laboratory setting. The experimental work was carried out in the Research Department of Zoology, Thiagarajar College, Madurai during the year 2023. The entomopathogenic fungi were isolated from soil by Galleria bait method. The pathogenicity of the fungi was evaluated through dipping bioassays using three different concentrations of conidial suspensions (1x106, 1x107 and 1x108) and a negative control of 0.05% Tween 80. The experiments were repeated three times, each with 25 insects. The results showed that A. flavus was effective in killing the pest, with a mortality rate of 89.3% after 120 hours. The pathogenicity of A. flavus was further analyzed through histological studies. The GC-MS analysis of the ethyl acetate extract of A. flavus confirmed the presence of insecticidal compounds such as pthalic acid and Dibutyl phthalate. Results indicated that A. flavus shows promise as a biocontrol agent against P. solenopsis and could be considered for use in field applications.
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References
Adsul V B, Khatiwora E, Torane R C and Deshpande N R .2012. Isolation and characterization of substituted dibutyl phthalate from leaves of Ipomoea carnea stem. Chem Nat Compd 48:712–713.
Afzal M B S and Shad S A. 2015. Resistance inheritance and mechanism to emamectin benzoate in Phenacoccus solenopsis (Homoptera: Pseudococcidae). Crop Prot 71:60–65.
Ahmad M and Akhtar S. 2016. Development of resistance to insecticides in the invasive mealybug Phenacoccus solenopsis (Hemiptera: Pseudococcidae) in Pakistan. Crop Protection 8:96–102.
Almudena Ortiz-Urquiza , Nemat O Keyhani. 2013. Action on the surface: Entomopathogenic fungi versus the insect cuticle, Insects 4(3):357-74.
Amrit Sharma, Shaurav Sharma & Pankaj Kumar Yadav .2023. Entomopathogenic fungi and their relevance in sustainable agriculture: A review, Cogent Food & Agriculture 9:1.
Anam Mehwish Khanzada, Muhammad Ali Khanzada, Rehana Naz Syed and Abdul Mubeen Lodhi. 2021. Comparative effectiveness of entomopathogenic fungi against okra mealybug phenacoccus solenopsi. Pak J Bot 53(1):287-292.
Araujo J P and Hughes D P. 2016. Diversity of entomopathogenic fungi: which groups conquered the insect body. Adv Genet 94: 1–39.
Bazazo K G, Amira S H M, Ibrahim ismael A and Khatab. 2019. Molecular identification of the entomopathogen Aspergillus candidus and its pathogenicity to mealybug Phenacoccucs solenopsis (Hemiptera: pseudococcidae) in Egyptian sugar beet fields. Egypt J Plant Prot Res Inst 2(3):450 – 459.
Berestetskiy A, Hu Q. 2021. The Chemical Ecology Approach to Reveal Fungal Metabolites for Arthropod Pest Management. Microorganisms 9(7):1379.
Bushra Saddiq, Sarfraz Ali Shad, Muhammad Aslam, Mamuna Ijaz and Naeem Abbas. 2015. Monitoring resistance of Phenacoccus solenopsis Tinsley (Homoptera: Pseudococcidae) to new chemical insecticides in Punjab, Pakistan. Crop Protection 74: 24-29.
Cuthbertson A G and Audsley N. 2016. Further screening of entomopathogenic fungi and nematodes as control agents for Drosophila suzukii. Insects 7(2):24.
Jayanthi K, Ayyasamy A, Kempraj V, Aurade RM, Govindan S, Verghese A. Aspergillus flavus impairs antioxidative enzymes of Sternochetus mangiferae during mycosis. (2015). J Invertebr Pathol 124:73-7.
Karthi S, Vaideki K, Shivakumar M S, Ponsankar A, Thanigaivel A, Chellappandian M, Vasantha Srinivasan P, Muthu Pandian C K, Hunter W B, Senthil Nathan S. 2018. Effect of Aspergillus flavus on the mortality and activity of antioxidant enzymes of Spodoptera litura Fab. (Lepidoptera: Noctuidae) larvae. Pestic Biochem Physiol 149:54-60
Kumar, Santosh & Sultana, Riffat & Wagan, and Muhammad saeed. 2015. Impact of entomopathogenic fungi Aspergillus flavus on life history statistics of Hieroglyphus oryzivorus (Orthoptera: Acrididae). Sindh University Research Journal (Science Series) 43: 493-496.
Madiha Nawaz and Shoaib Freed. 2022. Pathogenicity of different isolates of entomopathogenic fungi on cotton mealybug, Phenaccocus solenopsis Tinsley. Pakistan J Zool 54(1):275-282.
Mandeep Kaur, Pooja Chadha1, Sanehdeep Kaur and Amarjeet Kaur. 2021. Aspergillus flavus induced oxidative stress and immunosuppressive activity in Spodoptera litura as well as safety for mammals. BMC Microbiology 21:180.
Muhammad S Waqas, Zuhua Shi, Tian-Ci Yi, Rong Xiao, Ali AZ, Shoaib Asem, SS Elabasy and Dao-Chao Jina. 2021. Biology, ecology, and management of cotton mealybug Phenacoccus solenopsis Tinsley (Hemiptera: Pseudococcidae). Pest Manag Sci 237.
Murugan Arunthirumeni, Gunasekar Vinitha, Muthugounder Subramanian Shivakumar. 2023. Antifeedant and larvicidal activity of bioactive compounds isolated from entomopathogenic fungi Penicillium sp. for the control of agricultural and medically important insect pest (Spodoptera litura and Culex quinquefasciatus), Parasitology International 92.
Nagrare VS, Babasaheb B, Fand China Babu Naik, Bhausaheb naikwadi, Vrushali Deshmukh and Dharmendra sinh. 2019. Resistance development in cotton mealybug, Phenacoccus solenopsis Tinsley (Hemiptera: Pseudococcidae) to insecticides from Organophosphate, Thiadiazines and thiourea derivatives. Int J Trop insects Sci 10.
Priyanka Zala, Swati Jayswal and Bharat Maitreya. 2021. A review on cotton mealybug (Phenacoccus solenopsis Tinsley) and their management by botanical based insecticides. Research and Reviews: Journal of Environmental Sciences 3: 1-15.
Rajamanikyam M, Vadlapudi V, Parvathaneni SP, Koude D, Sripadi P, Misra S, Amanchy R, Upadhyayula SM. 2017. Isolation and characterization of phthalates from Brevibacterium mcbrellneri that cause cytotoxicity and cell cycle arrest, EXCLI J 217.
Sardul Singh Sandhu, Anil K Sharma, Vikas Beniwal, Gunjan Goel, Priya Batra, Anil Kumar, Sundeep Jaglan, Sharma A K and Sonal Malhotra. 2012. Myco-Biocontrol of insect pests: factors involved, mechanism, and regulation. Journal of Pathogens 1-10.
Singh D, Son SY and Leen CH. 2016. Perplexing metabolomes in fungal-insect trophic interactions: a terra incognita of mycobiocontrol mechanisms. Front Microbio 7: 1–13.
Tong Hao-jie, Ao Yan, LI Zi-hao, Wang Ying and Jiang Ming-xing. 2019. Invasion biology of the cotton mealybug, Phenacoccus solenopsis Tinsley: Current knowledge and future directions. Journal of Integrative Agriculture 18(4):758–770.
Ullah I, Khan AL, Ali L, Khan AR, Waqas M, Lee IJ, Shin JH. 2014.An insecticidal compound produced by an insect-pathogenic bacterium suppresses host defenses through phenoloxidase inhibition. Molecules 19(12):20913-28.
Vennila S, Deshmukh A, Pinjarkar D, Agarwal M, Ramamurthy W and Joshi S. 2010. Biology of the mealybug, Phenacoccus solenopsis on cotton in the laboratory. J of Insect Sci 10:115.
Wang JR, Li DQ, Xiong SH, Wei LL, Li SG, Luo GH, Shao B, Yang Z L. 2015. The host of Phenacoccus solenopsis in China mainland and a new record of Rubiaceae host. Plant Quarantine 29: 27–31.
Xu H and He X Q. 2010. Design, semisynthesis, and insecticidal activity of novel monomethyl phthalate derivatives of podophyllotoxin against Mythimna separata Walker in vivo. Bioorg. Med. Chem. Lett 20: 4503–4506.
Zimmermann G. 1986. The ‘Galleria bait method’ for detection of entomopathogenic fungi in soil. J. Appl. Entomol 102: 213–215.
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