Management of cucumber wilt using the potential native biocontrol agents

GURVEER SINGH  BRAR; NARINDER  SINGH; DALJEET  SINGH BUTTAR; AJAY KUMAR  CHOUDHARY

doi:10.56093/ijas.v95i9.150802

Authors

GURVEER SINGH BRAR Punjab Agricultural University, Ludhiana, Punjab 141 004, India
NARINDER SINGH Punjab Agricultural University, Ludhiana, Punjab 141 004, India
DALJEET SINGH BUTTAR Punjab Agricultural University, Ludhiana, Punjab 141 004, India
AJAY KUMAR CHOUDHARY Punjab Agricultural University, Ludhiana, Punjab 141 004, India

https://doi.org/10.56093/ijas.v95i9.150802

Keywords:

Cucumber wilt, Fusarium oxysporum f.sp. cucumerinum, Pseudomonas fluorescens, Trichoderma asperellum

Abstract

Cucumber wilt is a serious disease in cucumber (Cucumis sativus L.) cultivation which is caused by Fusarium oxysporum f. sp. cucumerinum (FOC). The field experiments were conducted during 2020–21 and 2021–22 at Punjab Agricultural University, Ludhiana, Punjab to evaluate the native potent isolates of Pseudomonas and Trichoderma against FOC. Among all the Pseudomonas fluorescens, Pf 6 showed maximum inhibition in dual culture (13.30 mm) assays against FOC. Similarly, in biochemical analysis, Pf 6 showed the highest chitinase activity and siderophore production, i.e. 11.56 unit/ml and 32.17 mm, respectively. Likewise, Trichoderma asperellum Thd emerged as the most effective BCA out of various Trichoderma isolates in the in vitro. The bio-efficacy bio formulations of P. fluorescens (Pf 6) and T. asperellum (Th d) were evaluated during 2020–2021 and 2021–2022. Among all the treatments, Pf 6 as seed + soil treatment showed minimum disease incidence (10.0% and 13.33%) along with enhanced plant growth and yield. Therefore, based on our findings, P. fluorescens (Pf 6) is an effective biocontrol agent and proved as an alternative approach for the effective management wilt of cucumber.

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