Multiple associations of Alternaria species on high density apple (Malus × domestica) plantation system in Jammu and Kashmir, India

MAHAPARA  RASHID; SUHAIL QUYOOM  WANI; ZAHOOR AHMAD  BHAT; MEHRAJ-UD-DIN  SHAH; MOHD MAQBOOL  MIR; IMRAN  KHAN; F A  LONE; NAJMU  SAKIB; MAHREENA FAROOQ  RATHER; DAYIM  ZAFFAR; MUSHTAQ AHMAD  BHAT

doi:10.56093/ijas.v95i8.155250

Authors

MAHAPARA RASHID Sher-e-Kashmir University of Agricultural Sciences and Technology, Shalimar, Srinagar, Jammu and Kashmir 190 025, India
SUHAIL QUYOOM WANI Sher-e-Kashmir University of Agricultural Sciences and Technology, Shalimar, Srinagar, Jammu and Kashmir 190 025, India
ZAHOOR AHMAD BHAT Sher-e-Kashmir University of Agricultural Sciences and Technology, Shalimar, Srinagar, Jammu and Kashmir 190 025, India
MEHRAJ-UD-DIN SHAH Sher-e-Kashmir University of Agricultural Sciences and Technology, Shalimar, Srinagar, Jammu and Kashmir 190 025, India
MOHD MAQBOOL MIR Sher-e-Kashmir University of Agricultural Sciences and Technology, Shalimar, Srinagar, Jammu and Kashmir 190 025, India
IMRAN KHAN Sher-e-Kashmir University of Agricultural Sciences and Technology, Shalimar, Srinagar, Jammu and Kashmir 190 025, India
F A LONE Sher-e-Kashmir University of Agricultural Sciences and Technology, Shalimar, Srinagar, Jammu and Kashmir 190 025, India
NAJMU SAKIB Sher-e-Kashmir University of Agricultural Sciences and Technology, Shalimar, Srinagar, Jammu and Kashmir 190 025, India
MAHREENA FAROOQ RATHER Sher-e-Kashmir University of Agricultural Sciences and Technology, Shalimar, Srinagar, Jammu and Kashmir 190 025, India
DAYIM ZAFFAR Sher-e-Kashmir University of Agricultural Sciences and Technology, Shalimar, Srinagar, Jammu and Kashmir 190 025, India
MUSHTAQ AHMAD BHAT Sher-e-Kashmir University of Agricultural Sciences and Technology, Shalimar, Srinagar, Jammu and Kashmir 190 025, India

https://doi.org/10.56093/ijas.v95i8.155250

Keywords:

ATPase, BLASTn, Clade, Morphocultural, Pathogenicity

Abstract

The study was carried out during 2021–2022 at Sher-e Kashmir University of Agricultural Sciences and Technology, Shalimar, Srinagar, Jammu and Kashmir to evalaute symptomatological development of Alternaria leaf blotch and spot of apple (Malus × domestica Borkh.) cultivars. The target of concern was to discern the specific Alternaria spp. intricately linked to manifestation of Alternaria fruit and leaf disease in apple trees under high density plantation system. Similarly, morpho-cultural characteristics of seven isolates were studied. Further, protein coding plasma membrane ATPase gene was used and the amplicon sequences were uploaded to GenBank and the accession numbers acquired after submissions for the ATPase were OQ355499, OQ222078, OQ333060 - OQ333064. The ATPase gene delimited all the isolates into three clades in which Clade-I contain sequences of Alternaria tenuissima. Clade-II consists sequences of Alternaria alternata while as Clade-III contains sequences of Alternaria arborescens. According to morphocultural, pathogenicity and molecular identification, the pathogens, viz. Alternaria alternata, Alternaria tenuissima, and Alternaria arborescens were found associated with apple leaf disease in India.

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