Exogenous application of GA3 influences morphological traits and developmental transitions in Or gene introgressed Indian cauliflower (Brassica oleracea var. botrytis)

ANAMIKA  CHANDEL; SHRAWAN  SINGH; MANISHA  MANGAL; B S  TOMAR; SHIVANI  NAGAR; HARITHA  BOLLINEDI; BHAG CHAND  SHIVRAN; SUSHMA  SAGAR

doi:10.56093/ijas.v95i2.155633

Authors

ANAMIKA CHANDEL ICAR-Indian Agricultural Research Institute, New Delhi 110012, India
SHRAWAN SINGH ICAR-Indian Agricultural Research Institute, New Delhi 110012, India
MANISHA MANGAL ICAR-Indian Agricultural Research Institute, New Delhi 110012, India
B S TOMAR ICAR-Indian Agricultural Research Institute, New Delhi 110012, India
SHIVANI NAGAR ICAR-Indian Agricultural Research Institute, New Delhi 110012, India
HARITHA BOLLINEDI ICAR-Indian Agricultural Research Institute, New Delhi 110012, India
BHAG CHAND SHIVRAN ICAR-Indian Agricultural Research Institute, New Delhi 110012, India
SUSHMA SAGAR ICAR-Indian Agricultural Research Institute, New Delhi 110012, India

https://doi.org/10.56093/ijas.v95i2.155633

Keywords:

Developmental transitions, GA3 spray, Orange cauliflower, Synchronization in flowering

Abstract

The present study was carried out during the winter (rabi) season of 2021–22 and 2022–23 at ICAR-Indian Agricultural Research Institute, New Delhi aimed to study the impact of GA₃ spray on morphological traits and developmental transitions in Or gene introgressed CFOr (PKVA-1HM) and white CFWT (DC 18–19) genotypes of Indian cauliflower (Brassica oleracea var. botrytis L). Total of 24 treatment combinations comprising four GA₃ concentrations, viz. Go, Control; G1, 250 ppm; G2, 500 ppm and G3, 1000 ppm; two genotypes, CFOr (homozygous orange) and CFWT (white); three applications at different plant stages, viz. S₁, Vegetative stage (VS); S₂, VS + curd initiation stage (CIS) and S₃, VS + CIS + full curd stage (FCS) in factorial randomised block design (Factorial- RBD) with three replications. Observations were recorded for six morphological traits and five developmental transitions. Significant differences were found for all three factors, viz. GA₃ concentration, genotypes and plant stages. A spray of 500 ppm GA3 on CFOr and CFWT genotype at VS + CIS stage resulted in superior performance in morphological traits and developmental transitions. It resulted maximum stalk length (69.33 cm) in CFOr, however, it was statistically similar to 250 ppm GA₃ spray at VS + CIS stage in CFWT (69.83cm). Both levels resulted in synchronization in flowering time of CFOr (153 days) and CFWT (153.5 days). The findings highlight the role of GA3 application in CFOr and CFWT genotypes for improving morphological traits and synchronize flowering for hybrid seed production.

Downloads

Download data is not yet available.

References

Crisp P, Walkey D G A, Bellman E and Roberts E. 1975. A mutation affecting curd colour in cauliflower (Brassica oleracea var. botrytis L.). Euphytica 24: 173–76.

Duclos D V and Bjorkman T. 2015. Gibberellin control of reproductive transitions in Brassica oleracea curd development. Journal of the American Society for Horticultural Science 140(1): 57–67.

Guo Y P, Liu Z and Yang C. 2004. Effects of gibberellin on inflorescence stalk elongation in vernalized and non-vernalized plants. Journal of Plant Growth Regulation 23(3): 200–10.

Hamano M, Yamato Y, Yamazaki H and Miura H. 2002. Endogenous gibberellins and their effects on flowering and stem elongation in cabbage (Brassica oleracea var. capitata). The Journal of Horticultural Science and Biotechnology 77(2): 220–25.

Hedden P and Sponsel V. 2015. A century of gibberellin research. Journal of Plant Growth Regulation 34(4): 740–60.

Jiang X, Li H, Wang T, Peng C, Wang H, Wu H and Wang X. 2012. Gibberellin indirectly promotes chloroplast biogenesis as a means to maintain the chloroplast population of expanded cells. The Plant Journal 72(5): 768–80.

Jung C, Muller A E and Amasino R M. 2020. Gibberellin promotes bolting and flowering in radish through integration with the vernalization pathway by activating the SOC1 gene and floral integrators FT and SOC1 under marginal vernalization. The Plant Journal 103(3): 1021–34.

Kalia P and Singh S. 2020. Accelerated improvement of cole vegetable crops. Accelerated Plant Breeding, Vol. 2, pp. 101–35. Gosal S and Wani S (Eds). Springer Nature, Singapore.

Kalia P, Muthukumar P and Soi S. 2018. Marker-assisted introgression of the Or gene for enhancing β-carotene content in Indian cauliflower. Acta Horticulturae 1203: 121–28.

Kalia P, Singh S, Selvakumar R, Mangal M and Nagarathna T K. 2023. Genome designing for nutritional quality in vegetable brassicas. Compendium of Crop Genome Designing for Nutraceuticals, pp. 1–97. Springer Nature, Singapore.

Kasahara H, Kamiya Y and Yamaguchi S. 2002. Biosynthesis of gibberellins and their role in plant development. Journal of Plant Research 115(3): 345–51.

Kaur P and Mal D. 2018. Effect of foliar spray of NAA and GA3 on the growth, curd formation and yield of cauliflower (Brassica oleracea var. botrytis L.). Journal of Pharmacognosy and Phytochemistry 7: 2805–07.

Kou E, Huang X, Zhu Y, Su W, Liu H, Sun G, Chen R, Hao Y and Song S. 2021. Crosstalk between auxin and gibberellin during stalk elongation in flowering Chinese cabbage. Scientific Reports 11: 3976.

Li L, Paolillo D J, Parthasarathy M V, DiMuzio E M and Garvin D F. 2001. A novel gene mutation that confers abnormal patterns of β-carotene accumulation in cauliflower (Brassica oleracea var. botrytis). The Plant Journal 26(1): 59–67.

Mandel R M, Rood S B and Pharis R P. 1992. Bolting and floral induction in annual and cold-requiring biennial Brassica spp.: Effects of photoperiod and exogenous gibberellin. Progress in Plant Growth Regulation, pp. 371–79.

Karssen C M, Vanloon L C and Vreugdenhil D (Eds). Kluwer Academic Publishers, Dordrecht, The Netherlands.

NHB. 2020. Second Advance Estimate on Area and Production of Horticultural Crops. All India National Horticulture Board, Gurugram, Haryana, India.

Nagar S, Singh V P, Singh N, Dhakar R, Arora A and Singh G P. 2022. Gibberellic acid mediated influence on physiological and yield-related traits under heat stress in wheat at reproductive stage. The Indian Journal of Agricultural Sciences 92(6): 700–04.

Nakajima K M, Ohishi M, Sato F and Takahashi M. 2023. Gibberellin-induced stem elongation and apical bud growth acceleration without decreased yield in broccoli (Brassica oleracea L. var. italica). Horticultural Journal 92(3): 281–89.

Prodhan M M, Sarker U, Hoque M A, Biswas M S, Ercisli S, Assouguem A, Ullah R, Almutairi M H, Mohamed H R H and Najda A. 2022. Foliar application of GA3 stimulates seed production in cauliflower. Agronomy 12: 1394.

Ramesh K and Kumar V. 2006. Gibberellin (GA3) stimulation of cell elongation, leaf area expansion, and overcoming dwarfism. Journal of Plant Growth Regulation 25(3): 180–85.

Singh R and Sharma P. 2003. Standard crop management practices and plant protection measures for cauliflower. Indian Journal of Horticulture 60(4): 375–82.

Singh S and Kalia P. 2021. Advances in cauliflower (Brassica oleracea var. botrytis L.) breeding, with emphasis on India. Advances in Plant Breeding Strategies: Vegetable Crops 10: 247–301.

Sonam, Singh S and Saxena A K. 2020. Efficacy of plant growth regulator (GA3) on growth and yield attributes of cauliflower (Brassica oleracea var. botrytis L.) at Dehradun valley. International Journal of Chemical Studies 8(5): 101–04.

Sun T P and Gubler F. 2004. Molecular mechanism of gibberellin signalling in plants. Annual Review of Plant Biology 55: 197–223.

Taiz L and Zeiger E. 2002. Plant Physiology, 3rd edn, pp. 1–690, Sinauer Associates, Sunderland, MA, USA.

Wang Q, Wang G L, Song S Y, Zhao Y N, Lu S and Zhou F. 2022. ORANGE negatively regulates flowering time in Arabidopsis thaliana. Journal of Plant Physiology 274: 153719.

Yacoub S and Abbas H. 2012. Effect of gibberellic acid on earliness of cauliflower curd initiation under assuit conditions. Assiut Journal of Agricultural Sciences 43(1): 45–54.