Effect of natural ageing on physiological and biochemical behavior of onion(Allium cepa) seeds having differential initial seed quality

NARENDER  PAL; SUDIPTA  BASU; ANJALI  ANAND; ANIL  KHAR; SHAILENDRA KUMAR  JHA; VISHWANATH  YALAMALLE; MONALISHA  SAHOO; SANDEEP KUMAR  LAL

doi:10.56093/ijas.v95i9.158482

Authors

NARENDER PAL ICAR-Indian Agricultural Research Institute, New Delhi, 110 012, India
SUDIPTA BASU ICAR-Indian Agricultural Research Institute, New Delhi, 110 012, India
ANJALI ANAND ICAR-Indian Agricultural Research Institute, New Delhi, 110 012, India
ANIL KHAR ICAR-Indian Agricultural Research Institute, New Delhi, 110 012, India
SHAILENDRA KUMAR JHA ICAR-Indian Agricultural Research Institute, New Delhi, 110 012, India
VISHWANATH YALAMALLE ICAR-Indian Agricultural Research Institute, New Delhi, 110 012, India
MONALISHA SAHOO ICAR-Indian Agricultural Research Institute, New Delhi, 110 012, India
SANDEEP KUMAR LAL ICAR-Indian Agricultural Research Institute, New Delhi, 110 012, India

https://doi.org/10.56093/ijas.v95i9.158482

Keywords:

Allium cepa L., Antioxidant enzymes, Lipid peroxidation NADPH oxidase, Natural ageing, Reactive oxygen species

Abstract

Onion (Allium cepa L.) seeds are short-lived and the seed quality deteriorates during storage. The understanding of seed deterioration associated with the ROS content along with the antioxidant enzyme activities specifically in relation with initial seed germination potential is not available. A study was carried out during 2022 and 2023 at ICAR-Indian Agricultural Research Institute, New Delhi to assess the biochemical behaviour of onion seeds having differential initial germination percentage, under ambient storage condition. Freshly harvested seeds of four onion genotypes i.e. Sukhsagar, POS 24K, Bhima Super and Bhima Shubhra, having different initial seed germination percentage ranging from 84–96 % were stored under ambient conditions for 12 months. The seed germination declined, ranging from 49–77 % after 12 months with a percent decline in germination ranging from 19–46%. An increase in the activity of NADPH oxidase, ROS as well as malondialdehyde content and decline in antioxidant enzyme activity was observed in onion seeds after storage. A significant positive correlation was found among germination percentage, catalase, peroxidase and superoxide dismutase activity, whereas germination percentage was negatively correlated with superoxide radical, hydrogen peroxide, MDA content and NADPH oxidase activity. The study implicated the critical role of catalase, peroxidase and superoxide dismutase in ameliorating the negative impact of superoxide radical, hydrogen peroxide that causes deterioration with seed ageing without being affected by initial germination potential.

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