Influence of light spectral ratios on physiological and stomatal parameters of pak choi (Brassica rapa var. chinensis) grown in acontrolled vertical hydroponic system

MAHESH  R; MURTAZA  HASAN; M D  YEASIN; RAMYA SREE M

doi:10.56093/ijas.v95i12.159592

Authors

MAHESH R ICAR-Indian Agricultural Research Institute, New Delhi
MURTAZA HASAN ICAR-Indian Agricultural Research Institute, New Delhi
M D YEASIN ICAR-Indian Agricultural Statistics Research Institute, New Delhi
RAMYA SREE M ICAR-Indian Agricultural Research Institute, New Delhi

https://doi.org/10.56093/ijas.v95i12.159592

Keywords:

Net photosynthetic rate, Pak choi, Pigments, Stomata, Transpiration rate

Abstract

Light quality, specifically the ratio of red and blue wavelength LEDs, is critical in optimising plants' photosynthetic rate and related physiological processes for higher yield and quality. The present study was carried out during 2022 and 2023 at ICAR-Indian Agricultural Research Institute, New Delhi to investigate the effects of different red (R) and blue (B) light ratios (R2B1= 2:1, R1B1= 1:1 and R1B2= 1:2) on plants stomatal characteristics and physiological parameters. Significant variations were observed for stomatal pore width, length, and guard cell dimensions across treatments, particularly with R1B1 and control (white light) conditions. Notably, R1B1 demonstrated increased stomatal pore width and guard cell dimensions over time, while control maintained higher stomatal density on the abaxial surface at 30 days after treatment (DAT). Stomatal responses varied, with R2B1 achieving the highest stomatal density and physiological efficiency as evidenced by a net photosynthetic rate of 22.26 µmol CO₂/m²/s and a water-use efficiency (WUE) of 72.67. The control treatment showed the lowest values across all physiological parameters, highlighting the benefits of spectral treatments. Correlation analysis revealed significant positive relationship among the physiological and stomatal parameters, particularly between net photosynthetic rate and WUE (r = 0.97), stomatal conductance and guard cell arc length (r = 0.97). The yield was observed highest (113.61 g fresh weight and 6.98 g dry weight) in R2B1 treatment. These findings underscore the critical role of light quality in optimising stomatal function and enhancing photosynthesis, transpiration and water-use efficiency in plants, suggesting potential applications in controlled agricultural environments.

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References

Aires A. 2018. Hydroponic production systems: Impact on nutritional status and bioactive compounds of fresh vegetables. (In) Vegetables: Importance of Quality Vegetables to Human Health. Asaduzzaman M D and Asao T (Eds). IntechOpen, London. DOI: https://doi.org/10.5772/intechopen.73011

Ando E and Kinoshita T. 2018. Red light-induced phosphorylation of plasma membrane H +-ATPase in stomatal guard cells. Plant Physiology 178: 838–49. DOI: https://doi.org/10.1104/pp.18.00544

Assmann S M and Shimazaki Ki. 1999. The multisensory guard cell. Stomatal responses to blue light and abscisic acid. Plant Physiology 119: 809–16. DOI: https://doi.org/10.1104/pp.119.3.809

Ballard T, Peak D and Mott K. 2019. Blue and red light effects on stomatal oscillations. Functional Plant Biology 46: 146–51. DOI: https://doi.org/10.1071/FP18104

Cartea M E, Francisco M, Soengas P and Velasco P. 2011. Phenolic compounds in Brassica vegetables. Molecules 16: 251–80. DOI: https://doi.org/10.3390/molecules16010251

Chen J, Wu J H, Si H P and Lin K Y. 2016. Effects of adding wood vinegar to nutrient solution on the growth, photosynthesis, and absorption of mineral elements of hydroponic lettuce. Journal of Plant Nutrition 39: 456–62. DOI: https://doi.org/10.1080/01904167.2014.992539

Cotula L, Toulmin C and Quan J. 2006. Better Land Access for the Rural Poor: Lessons from Experience and Challenges Ahead. International Institute for Environment and Development, FAO. Cubo Ribas N. 2021. ‘Trade-off between photosynthesis and stress tolerance: The role of foliar anatomy and the antioxidant system’. MSc Thesis, Centre for Postgraduate Studies, University of the Balearic Islands.

Gao Q, Liao Q, Li Q, Yang Q, Wang F and Li J. 2022. Effects of LED red and blue light component on growth and photosynthetic characteristics of coriander in plant factory. Scientia Horticulturae 8: 1165. DOI: https://doi.org/10.3390/horticulturae8121165

Hiscox J D and Israelstam G F. 1979. A method for the extraction of chlorophyll from leaf tissue without maceration. Canadian Journal of Botany 57: 1332–34. DOI: https://doi.org/10.1139/b79-163

Huang J, Xu Y L, Duan F M, Du X, Yang Q C and Zheng Y J. 2021. Improvement of the growth and nutritional quality of two-leaf-colour pak choi by supplemental alternating red and blue light. HortScience 56: 118–25. DOI: https://doi.org/10.21273/HORTSCI15180-20

Inada K. 1976. Action spectra for photosynthesis in higher plants. Plant Cell Physiology 17: 355–65.

Kaiser E, Morales A and Harbinson J. 2018. Fluctuating light takes crop photosynthesis on a rollercoaster ride. Plant Physiology 176: 977–89. DOI: https://doi.org/10.1104/pp.17.01250

Kaiser H and Kappen L. 2000. In situ observation of stomatal movements and gas exchange of Aegopodium podagraria L. in the understorey. Journal of Experimental Botany 51: 1741–49. DOI: https://doi.org/10.1093/jexbot/51.351.1741

Kumar M, Bassi N, Narayanamoorthy A and Sivamohan M. 2014. The Water, Energy and Food Security Nexus: Lessons from India for Development, 1st edn. Routledge, Abingdon. DOI: https://doi.org/10.4324/9781315848686

Lanoue J, Leonardos E D and Grodzinski B. 2018. Effects of light quality and intensity on diurnal patterns and rates of photo-assimilate translocation and transpiration in tomato leaves. Frontiers in Plant Science 9: 756. DOI: https://doi.org/10.3389/fpls.2018.00756

Lawson T. 2009. Guard cell photosynthesis and stomatal function. New Phytologist 181: 13–34. DOI: https://doi.org/10.1111/j.1469-8137.2008.02685.x

Lawson T, Terashima I, Fujita T and Wang Y. 2018. Coordination between photosynthesis and stomatal behavior. (In) The Leaf: A Platform for Performing Photosynthesis, pp. 141–61. Adams WW III and Terashima I (Eds). Springer, Cham. DOI: https://doi.org/10.1007/978-3-319-93594-2_6

Matthews J S A, Vialet-Chabrand S and Lawson T. 2018. Acclimation to fluctuating light impacts the rapidity of response and diurnal rhythm of stomatal conductance. Plant Physiology 176: 1939–51. DOI: https://doi.org/10.1104/pp.17.01809

Matthews J S, Vialet-Chabrand S and Lawson T. 2020. Role of blue and red light in stomatal dynamic behaviour. Journal of Experimental Botany 71: 2253–69. DOI: https://doi.org/10.1093/jxb/erz563

Sardare M and Admane S. 2013. A review on plant without soil-Hydroponics. International Journal of Research in Engineering and Technology 2(3): 299–304. DOI: https://doi.org/10.15623/ijret.2013.0203013

Serrano E E, Zeiger E and Hagiwara S. 1988. Red light stimulates an electrogenic proton pump in Vicia guard cell protoplasts. Proceedings of the National Academy of Sciences, USA 85: 436–40. DOI: https://doi.org/10.1073/pnas.85.2.436

Sharkey T D and Raschke K. 1981. Effect of light quality on stomatal opening in leaves of Xanthium strumarium L. Plant Physiology 68(5): 1170–74. DOI: https://doi.org/10.1104/pp.68.5.1170

Sharma N, Acharya S, Kumar K, Singh N and Chaurasia O P. 2018. Hydroponics as an advanced technique for vegetable production: An overview. Journal of Soil and Water Conservation 17(4): 364–71. DOI: https://doi.org/10.5958/2455-7145.2018.00056.5

Shimazaki K I, Doi M, Assmann S M and Kinoshita T. 2007. Light regulation of stomatal movement. Annual Review of Plant Biology 58: 219–47. DOI: https://doi.org/10.1146/annurev.arplant.57.032905.105434

Tominaga M, Kinoshita T and Shimazaki K. 2001. Guard-cell chloroplasts provide ATP required for H+ pumping in the plasma membrane and stomatal opening. Plant and Cell Physiology 42: 795–802. DOI: https://doi.org/10.1093/pcp/pce101

Wiangsamut B and Koolpluksee M. 2020. Yield and growth of pak choi and green oak vegetables grown in substrate plots and hydroponic systems with different plant spacing. International Journal of Agricultural Technology 16(4): 1063–76.

Zeiger E. 1983. The biology of stomatal guard cells. Annual Review of Plant Physiology and Plant Molecular Biology 34: 441–75. Zhou F, Zuo J, Xu D, Gao L, Wang Q and Jiang A. 2020. Low-intensity white light-emitting diodes (LED) application to delay senescence and maintain quality of postharvest pakchoi [Brassica campestris L. ssp. chinensis (L.) Makino var. communis Tsen et Lee]. Scientia Horticulturae 262: 109060. DOI: https://doi.org/10.1016/j.scienta.2019.109060