Management of root-knot nematode, Meloidogyne incognita infesting black pepper (Piper nigrum) under field conditions


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Authors

  • RAJKUMAR ICAR-Central Plantation Crops Research Institute, Kasaragod, Kerala 671 124, India image/svg+xml
  • RASHID PERVEZ ICAR-Indian Agricultural Research Institute, New Delhi, India image/svg+xml
  • R SUREKHA ICAR-Central Plantation Crops Research Institute, Kasaragod, Kerala 671 124, India image/svg+xml

https://doi.org/10.56093/ijas.v96i02.161140

Keywords:

Black pepper, Carbosulfan, Nematode management, Pochonia chlamydosporia

Abstract

The present experiment were conducted during 2020, 2021 and 2022 at the ICAR-Central Plantation Crops Research Institute, Kasaragod, Kerala for the management of the root-knot nematode, Meloidogyne incognita, infesting black pepper (Piper nigrum L.) cv. Panniyur-1 using a talc-based formulation of Trichoderma harzianum [ICAR-Indian Institute of Spice Research (ICAR-IISR) and ICAR-Central Plantation Crop Research Institute (ICAR-CPCRI) isolates] and Pochonia chlamydosporia (ICAR-IISR isolate), neem cake, and carbosulfan 25 ec (standard check). The experiment was laid out in a randomised block design (RBD). The results indicated that P. chlamydosporia (84 and 72%, respectively) and carbosulfan (91 and 79%, respectively) both exhibited the highest decreases in M. incognita populations in the soil and root, respectively compared to the control. Maximum yield was recorded in carbosulfan (25.86 kg) and P. chlamydosporia (24.32 kg) treated plants in comparison to the control (9.07 kg). P. chlamydosporia (ICAR-IISR isolate) was found promising against M. incognita to reduce the population buildup in the fields, thus, indicating that there exists a scope for management of nematode diseases for organic production of black pepper and better yield to sustain the additional income of the farmer.

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References

Aranda-Martinez A, Lenfant N, Escudero N, Zavala-Gonzalez E A, Henrissat B and Lopez-Llorca L V. 2016. CAZyme content of Pochonia chlamydosporia reflects that chitin and chitosan modification are involved in nematode parasitism. Environmental Microbiology 18(11): 4200–15.

Barker K R, Carter C C and Sasser J N. 1985. Nematode extraction and bioassays. (In) An Advanced Treatise on Meloidogyne: Biology and Control, Vol. 2, pp. 19–35. North Carolina State University, Raleigh, USA.

Bhai R S, Eapen S J, Kumar A, Aravind R, Pervez R, Varghese E M, Krishna P B and Sreeja K. 2017. Mitigating Phytophthora foot rot and slow decline diseases of black pepper through the deployment of bacterial antagonists. Journal of Spices and Aromatic Crops 26(2): 69–82.

Bordallo J J, Lopez-Llorca L V, Jansson H B, Salinas J, Persmark L and Asensio L. 2002. Colonization of plant roots by egg-parasitic and nematode-trapping fungi. New Phytologist 154: 491–99.

Chitwood D J. 2003. Research on plant-parasitic nematode biology conducted by the United States Department of Agriculture- Agriculture Research Service. Pest Management Science 59: 748–53.

El-Nagdi W M A, Youssef M M A, El-Khair H A and Abd-Elgawad M M M. 2019. Effect of certain organic amendments and Trichoderma species on the root-knot nematode, Meloidogyne incognita, infecting pea (Pisum sativum L.) plants. Egyptian Journal of Biological Pest Control 29: 75.

Eisenback J D. 1985. Diagnostic characters useful in the identification of the four most common species of root-knot nematodes (Meloidogyne spp.). (In) An Advanced Treatise on Meloidogyne, 1 Biology and Control, pp. 95–112. Sasser J N and Carter C C (Eds). North Carolina State University, Raleigh, USA.

Ghahremani Z, Escudero N, Saus E, Gabaldon T and Sorribas F J. 2019. Pochonia chlamydosporia induces plant-dependent systemic resistance to Meloidogyne incognita. Frontiers in Plant Science 10: 945.

Gharabadiyan F, Jamali S, Yazdi A A, Hadizadeh M H and Eskandari A. 2012. Weed hosts of root-knot nematodes in tomato fields. Journal of Plant Protection Research 52(2): 230–34.

Hooper D J, Hallmann J and Subbotin S A. 2005. Methods for extraction, processing and detection of plant and soil nematodes. (In) Plant Parasitic Nematodes in Subtropical and Tropical Agriculture, pp. 53–86. Luc M, Sikora R A and Bridge J (Eds). CABI Publishing, Wallingford, UK.

Jepson S B. 1987. Identification of Root-Knot Nematodes (Meloidogyne spp.), pp. 265. CAB International, Wallingford, United Kingdom.

Khan A, Ahmad G, Haris M and Khan A A. 2022. Bio-organics management: Novel strategies to manage root-knot nematode, Meloidogyne incognita pest of vegetable crops. Gesunde Pflanzen 75: 193–209.

Kumar V, Khan M R and Walia R K. 2020. Crop loss estimations due to plant-parasitic nematodes in major crops on India. National Academy of Scientific Letters 43(5): 409 –12.

Larriba E, Jaime M D L A, Nislow C, Martín-Nieto J and Lopez- Llorca LV. 2015. Endophytic colonization of barley (Hordeum vulgare) roots by the nematophagous fungus Pochonia chlamydosporia reveals plant growth promotion and a general defense and stress transcriptomic response. Journal of Plant Research 128: 665–78.

Leela N K, Pervez R, Rammanna V, Rosana O B and Eapen S J. 2012. Nematicidal activity of Strychnos nuxvomica leaf and its constituents against the root-knot nematode, Meloidogyne incognita. Nematologia Mediterranea 40(2): 116–23.

Macia-Vicente J G, Rosso L C, Ciancio A, Jansson H B and Lopez-Llorca L V. 2009. Colonization of barley roots by endophytic Fusarium equiseti and Pochonia chlamydosporia: Effects on plant growth and disease. Annals of Applied Biology 155: 391–401.

Manzanilla-Lopez R H, Esteves I, Powers S J and Kerry B R. 2011. Effects of crop plants on abundance of Pochonia chlamydosporia and other fungal parasites of root-knot and potato cyst nematode. Annals of Applied Biology 159: 118–29.

Medeiros H A, Araujo Filho J V, Freitas L G, Castillo P, Rubio M B, Hermosa R and Monte E. 2017. Tomato progeny inherit resistance to the nematode Meloidogyne javanica linked to plant growth induced by the biocontrol fungus Trichoderma atroviride. Scientific Reports 7: 40216.

Monteiro T S A, Monteiro T A, Pacheco P V M, Gouveia A S, Balbino H M and Freitas L G 2020. (In) Pochonia, Beneficial Microbes in Agro-Ecology: Bacteria and Fungi. Amaresan N, Senthil K M, Annapurna K, Kumar K and Sankaranarayanan A (Eds). Elsevier, Academic Press.

Munif A, Hallmann J and Sikora R A. 2000. Evaluation of the biocontrol activity of endophytic bacteria from tomato against Meloidogyne incognita. Mededelingen Faculteit Landbouwwetenschappen Universiteit Gent 65: 471–80.

Patil J A, Yadav S and Kumar A. 2021. Management of root-knot nematode, Meloidogyne incognita and soil borne fungus, Fusarium oxysporum in cucumber using three bioagents under polyhouse conditions. Saudi Journal of Biological Sciences 28(12): 7006–11.

Pentimone I, Lebron R, Hackenberg M, Rosso L C, Colagiero M and Nigro F. 2018. Identification of tomato miRNAs responsive to root colonization by endophytic Pochonia chlamydosporia. Applied Microbiology and Biotechnology 102: 907–19.

Pervez R and Eapen S J. 2015. Distribution of plant parasitic nematodes associated with black pepper rhizosphere from Idukki district (Kerala), India. Annals of Plant Protection Sciences 23(1): 192–93.

Pervez R and Eapen S J. 2016. Community structure of plant parasitic nematodes associated with black pepper in Idukki district (Kerala), India. Current Nematology 27(1): 73–76.

Pervez R and Eapen S J. 2021. Management of burrowing nematode, Radopholus similis (Cobb, 1893) Thorne, 1949 in black pepper nurseries. Indian Journal of Nematology 51(2): 175–80.

Pervez R, Eapen S J, Jacob T K, Hamza S and Srinivasan V. 2016. Diversity and community analysis of nematodes associated with Piper nigrum from Idukki District (Kerala), India. Indian Journal of Nematology 46(1): 7–13.

Pervez R, Niraul I M and Eapen S J. 2017. Hirschmanniella spp. (Nematoda: Pratylenchidae) associated with black pepper (Piper nigrum L.): A first report. Annals of Plant Protection Sciences 24(2): 385–91.

Pervez R, Rajkumar, Eapen S J and Surekha R. 2024. Management of root-knot nematode, Meloidogyne incognita infecting turmeric (Curcuma longa L.) under coconut cropping system. The Indian Journal of Agricultural Sciences 94(4): 422–26.

Pervez R. 2017. Plant parasitic nematodes: Hidden enemy of black pepper (Piper nigrum L.). Indian Journal of Arecanut, Spices and Medicinal Plants 19(4): 24–28.

Prasad L, Pervez R and Swati G. 2021. Bio-efficacy of fungal bioagents and its crude secondary metabolites against root knot nematode Meloidogyne incognita (Kofoid & White, 1919) Chitwood, 1949 infesting tomato (Solanum lycopersicum L.). REDIA 104: 37–43.

Rajkumar, Pervez R, Surekha R and Bhat R. 2019. Weeds as alternate hosts of root-knot nematode, Meloidogyne incognita in coconut garden. Annals of Plant Protection Sciences 27(2): 285–88.

Ramana K V and Eapen S J. 2000. Nematode induced diseases of black pepper. (In) Black Pepper Piper nigrum, pp. 269– 96. Ravindran P N (Ed). Amsterdam, Harwood Academic Publishers.

Rao M S. 2008. Management of Meloidogyne javanica on acid lime using Paecilomyces lilacinus and Pseudomonas fluo rescens. Nematologia Mediterranea 36: 45–50.

Sarr P S, Yamakawa T, Asatsuma S, Fujimoto S and Sakai M. 2010. Investigation of endophytic and symbiotic features of Rasltonia spp. TSC1 isolated from cowpea nodules. African Journal of Microbiology Resistance 4: 1959–63.

Seinhorst J W. 1959. A rapid method for the transfer of nematodes from fixative to anhydrous glycerin. Nematologica 4: 67–69.

Sharon E, Bar E M, Chet I, Herrera E A, Kleifeld O and Spiegel Y. 2001. Biological control of the root-knot nematode (M. javanica) by T. harzianum. Phytopathology 91(7): 687–93.

Siddiqi M R. 2000. Tylenchida-Parasites of Plants and Insects, 2nd edn, pp. 852. CABI Publishing, London, UK.

Singh S. 2013. Integrated approach for the management of the root-knot nematode, Meloidogyne incognita on eggplant under field conditions. Nematology 15: 747–57.

Southey J F. 1985. Laboratory Methods for Work with Plant and Soil Nematodes. Her Majesty’s Stationary Office, London, UK.

Spice Board. 2023. https://indianspices.com/sites/default/ files/Major%20spice%20state% 20wise% 20area%20 production%202022-23.pdf

Taylor A L and Sasser J N 1978. Biology, Identification and Control of Root-Knot Nematodes (Meloidogyne species), pp. 111. North Carolina State University Graphics, USA.

Thomas L and Rajeev P. 2015. Black Pepper-Extension Pamphlet, pp. 27. ICAR-Indian Institute of Spices Research, Kozhikode, Kerala.

Uddin M N, Saifullah S, Ahmad M, Khan W and Khan B M. 2019. Evaluation of Pochonia chlamydosporia (Goddard) isolates for suppression of Meloidogyne incognita, root-knot nematode of tomato. Journal of Agricultural Science 11: 70–81.

Vetrivelkalai P, Sivakumar M and Jonathan F I. 2010. Biocontrol potential of endophytic bacteria on Meloidogyne incognita and its effect on plant growth in bhendi. Journal of Bio-pesticides 3: 452–57.

Yang C J, Zhang X G, Shi G Y, Zhao H Y, Chen L, Tao K and Hou T P. 2011. Isolation and identification of endophytic bacterium W4 against tomato Botrytis cinerea and antagonistic activity stability. African Journal of Microbiological Resistance 5: 131–36.

Yi X, Guo Y, Khan R A A and Fan Z. 2021. Understanding the pathogenicity of Pochonia chlamydosporia to root-knot nematode through omics approaches and action mechanism. Biological Control 162: 104726.

Zavala-Gonzalez E A, Rodriguez-Cazorla E, Escudero N, Aranda- Martinez A, Martínez-Laborda A and Ramirez-Lepe M. 2017. Arabidopsis thaliana root colonisation by the nematophagous fungus Pochonia chlamydosporia is modulated by jasmonate signaling and leads to accelerated flowering and improved yield. New Phytologist 213: 351–64.

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2024-11-27

Published

2026-02-20

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How to Cite

RAJKUMAR, PERVEZ, R. ., & SUREKHA, R. . (2026). Management of root-knot nematode, Meloidogyne incognita infesting black pepper (Piper nigrum) under field conditions. The Indian Journal of Agricultural Sciences, 96(2), 250–255. https://doi.org/10.56093/ijas.v96i02.161140
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