In vitro propagation of almond (Prunus dulcis) cv. Merced

M A MIR; K M BHAT; Z A RATHER; F A P; A H PANDIT; G Hussain

doi:10.56093/ijas.v82i4.16632

Authors

M A MIR Sher-e-Kashmir University of Agricultural Sciences and Technology of Kashmir, Shalimar, Jammu and Kashmir 191 121
K M BHAT Sher-e-Kashmir University of Agricultural Sciences and Technology of Kashmir, Shalimar, Jammu and Kashmir 191 121
Z A RATHER Sher-e-Kashmir University of Agricultural Sciences and Technology of Kashmir, Shalimar, Jammu and Kashmir 191 121
F A P Sher-e-Kashmir University of Agricultural Sciences and Technology of Kashmir, Shalimar, Jammu and Kashmir 191 121
A H PANDIT Sher-e-Kashmir University of Agricultural Sciences and Technology of Kashmir, Shalimar, Jammu and Kashmir 191 121
G Hussain Sher-e-Kashmir University of Agricultural Sciences and Technology of Kashmir, Shalimar, Jammu and Kashmir 191 121

https://doi.org/10.56093/ijas.v82i4.16632

Keywords:

Almond, Growth regulators, Micro propagation, Proliferation, Rooting

Abstract

Forced and unforced shoot tips were surface sterilized with different sterilant regimes and incubated under normal culture room conditions. Surface sterilization of explants with mercuric chloride 0.1% (w/v) for 10 min. was found effective in improving culture asepsis (51.66%) and explant survival (55.00%). Higher values for both these parameters were recorded with forced explants in comparison to unforced ones. Main effect of growth regulators and media was significant on explant establishment which was maximum (66.66%) on ½ MS media containing BAP + IBA (0.50+0.01 mg/l). Callusing at the base of initiating cultures was minimum (24.58%) with BAP+IBA (0.25+0.01 mg/l). Microshoots from the established cultures were subcultured on the MS media supplemented with BAP and NAA alone or in combination for axillary shoot proliferation. Maximum proliferated cultures (86.66%) with maximum shoot number/explant (15.61) and proliferation grade (4.00) was obtained with BAP+NAA ( 0.40 + 0.01 mg/l). BAP was found superior to NAA during axillary shoot proliferation. Microshoots (10–15 mm) from proliferated cultures were subcultured in root induction medium (MS medium supplemented with IBA) and incubated under darkness for 10 days at 24±1 oC and then transferred to root development medium (hormone-free MS medium) and incubated under normal culture room conditions. Highest rooting of microshoots (93.33%) with maximum root number/shoot (5.90) and root length (43.00 mm) was obtained with IBA (1.0 mg/l).

Downloads

Download data is not yet available.

References

Ainsley P J, Collins G G and Sedgley M. 2001. In vitro rooting of almond (Prunus dulcis Mill.). In vitro Cellular and Developmental Biology- Plant 37: 778–85.

Bouza L. 1997. Micropropagation of Prunus tenella (dwarf Russian almond). (in) Biotechnology in Agriculture and Forestry, Vol.

VI pp 276–88. Bajaj Y P S (Ed.). Springer-Verlag, Berlin Heidelberg.

Cidem I, Filiz A A, Sureyya N, Engin T and Davut B. 2008. In vitro micropropagation of almond (Amygdalus communis L. cv. Non Pariel). African Journal of Biotechnology. 7(12): 1875–80.

Dalal M A, Kuchey A G and Rather M A. 2000. Studies on stock plant treatment and culture initiation in control of browning and media exudation in in vitro cultures of apple (Malus domestica Borkh.). SKUAST Journal of Research 2: 10–16.

Filiz A A, Cidem I, Sureyya N and Bekir E. 2009. Effect of plant growth regulators on in vitro shoot multiplication of Amygdalus communis L. cv. Yaltsinki. African Journal of Biotechnology. 8(22): 6168–74.

Hammerschlag F A. 1980. Peach micropropagation. Agricultural Research Results. pp. 48–52, ARR-NE 118, Beltsville.

Kassim N E, Abou Rayga S M and Ali E A M. 2010. Effect of explant types and different nutrient media on in vitro growth of bitter almond cuttings during establishment and proliferation stages. Journal of American Science 6(9): 340–3.

Kester D E, Liu F, Fenton C A L and Durzan D J. 1986. Almond [Prunus dulcis (Miller) D A Webb.]. (in) Biotechnology in Agriculture and Forestry, Vol. I, pp 414–30 Bajaj Y P S (Ed.). Springer-Verlag, Berlin, Heidelberg.

Peer F A, Farooqui K D, Dar K R, Bhat M Y, Hussain G and Rather Z A. 2011. In vitro propagation of sweet cherry (Prunus avium L) rootstock cv. ‘Mazzard’. Applied Biological Research. 13(1): 10–6

Qadiri I H, Kamili A N and Shah A M. 2001. Micropropagation of almond. Journal of Research and Development 1: 111–7.

Qadiri I H, Kamili A N, Bashir S and Shah A M. 2002. Shoot tip culture of thin shelled almond. Oriental Science 7: 95–102.

Rugini E and Verma D C. 1982. Micropropagation and cell suspensions of a difficult to propagate almond (Prunus amygdalus Batsch.) cultivar. (in) Proceedings of 5th International Conference on Plant Tissue and Cell Culture, pp 741–2. A Fujiwara (Ed.). The Japanese Association for Plant Tissue Culture, Tokyo.