Synergistic effects of bio agents and botanicals for integrated management of rice root-knot nematode, Meloidogyne graminicola
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Keywords:
Annona oil, Bacillus subtilis, Garlic oil, Management, Polythene cover, Root-knot nematodeAbstract
This research investigates the efficacy of multiple treatment methods in controlling Meloidogyne graminicola, the rice root-knot nematode, under field conditions. The treatments tested included the nematicide Fluensulfone (trade name Nimitz) @ 5g/block, bioagent Bacillus subtilis @ 15g/plot (CFU 1.5 x 109), and the botanicals garlic oil @ 2000 ppm and annona oil @ 2000 ppm; these were applied separately and in combination. The experiment was conducted in a sick plot at the Division of Nematology, ICAR-IARI, New Delhi, with and without polythene sheet covering. All the observations were made at 60 days post-treatment, focusing on plant height and the number of galls per seedling. Results showed that the combined application of garlic oil and B. subtilis significantly reduced nematode infestation, with the lowest gall count (9.14/plant) and the tallest seedlings (48.8 cm), especially under a polythene sheet covering. Similar patterns were observed with other combinations of garlic oil and B. subtilis, showing notable increases in plant height and decreases in gall counts. The influence was more significant with polythene covering compared to without polythene sheet covering.
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