Effect of natural farming practices on physiological and biochemical traits of cabbage (Brassica oleracea var. capitata) under mid-hill conditions of Himachal Pradesh

PANKAJ  VERMA; SANJEEV  KUMAR; RUPAM  NEHTA; IBAJANAI  KURBAH; GARIMA; RAJESHWAR SINGH  CHANDEL; ASHU  CHANDEL

doi:10.56093/ijas.v96i6.172673

Authors

PANKAJ VERMA Dr. Yashwant Singh Parmar University of Horticulture and Forestry, Nauni , Solan, Himachal Pradesh 173 230, India
SANJEEV KUMAR Dr. Yashwant Singh Parmar University of Horticulture and Forestry, Nauni , Solan, Himachal Pradesh 173 230, India
RUPAM NEHTA Dr. Yashwant Singh Parmar University of Horticulture and Forestry, Nauni , Solan, Himachal Pradesh 173 230, India
IBAJANAI KURBAH Dr. Yashwant Singh Parmar University of Horticulture and Forestry, Nauni , Solan, Himachal Pradesh 173 230, India
GARIMA Dr. Yashwant Singh Parmar University of Horticulture and Forestry, Nauni , Solan, Himachal Pradesh 173 230, India
RAJESHWAR SINGH CHANDEL Dr. Yashwant Singh Parmar University of Horticulture and Forestry, Nauni , Solan, Himachal Pradesh 173 230, India
ASHU CHANDEL Dr. Yashwant Singh Parmar University of Horticulture and Forestry, Nauni , Solan, Himachal Pradesh 173 230, India

https://doi.org/10.56093/ijas.v96i6.172673

Keywords:

Antioxidants, Biochemical quality, Jeevamrit, Physiological attributes, Sustainable agriculture

Abstract

Natural farming helps in improving sustainability; and it is imperative to know at which concentration of its inputs the crops respond best. Therefore, the field experiment was conducted to evaluate the effect of natural farming practices on cabbage (Brassica oleracea L. var. capitata) in the mid-hill region of Himachal Pradesh during two consecutive seasons (2023–24 and 2024–25). Six treatments, consisting of a control and five concentrations of jeevamrit (10%, 20%, 30%, 40% and 50%) were evaluated for physiological parameters including chlorophyll content, carotenoids, photosynthetic rate, transpiration rate, leaf area index as well as stomatal traits and biochemical characteristics such as total phenolics, flavonoids and antioxidant activity. The results revealed that all doses of jeevamrit significantly improved these parameters over control. Among various treatments, 40% dose recorded maximum chlorophyll content, higher transpiration rates, secondary metabolite accumulation and enhanced antioxidant activity. A reduction in values at 50% suggested that the response had surpassed the optimum concentration. Principal Component Analysis (PCA) and correlation analysis showed strong positive associations between phenolic compounds, flavonoids and antioxidant capacity (DPPH), as indicated by high correlation coefficients (r = 0.97–0.99) and close vector alignment in the PCA biplot. Phenolic and flavonoid levels were significantly correlated with antioxidant capacity at optimal levels, suggesting enhanced physiological performance and tolerance to stress conditions. In general, these results indicated that 40% jeevamrit is the most effective concentration for improving physiological performance and biochemical quality of cabbage under natural farming conditions.

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References

APEDA. 2024. India production data (HS Code 10712). AgriExchange, Agricultural and Processed Food Products, Export Development Authority, Ministry of Commerce and Industry, Govt. of India (Retrieved 5 June 2025).

Bertolino L T, Caine R S and Gray J E. 2019. Impact of stomatal density and morphology on water-use efficiency in a changing world. Frontiers in Plant Science 10: 225. DOI: https://doi.org/10.3389/fpls.2019.00225

Chandel R S and Verma S. 2024. Climate resilient natural farming practices for sustaining mountain agroecosystem: Status and scope. Journal of the Indian Society of Soil Science 72 (Special issue):112.

Chaudhary S K, Singh S K, Sinha N and Kumar V. 2024. Response of inorganic, integrated and organic sources of nutrients on growth and yield of cabbage. International Journal of Plant and Soil Science 36(8): 476–85. DOI: https://doi.org/10.9734/ijpss/2024/v36i84878

Chun O K, Smith N, Sakagawa A and Lee C Y. 2004. Antioxidant properties of raw and processed cabbages. International Journal of Food Science and Technology 39(5): 503–09. DOI: https://doi.org/10.1080/09637480410001725148

FAO. 2024. FAOSTAT: Production: Crops and Livestock Products. Food and Agriculture Organization, Rome, Italy. Available at:

https://www.fao.org/faostat/en/#data/QCL

Fukuoka M. 1975. The One-Straw Revolution. Hakujusha, Tokyo, Japan. 252p.

Khadse A, Rosset P M, Morales H and Ferguson B G. 2018. Taking agroecology to scale: The zero budget natural farming peasant movement in Karnataka, India. The Journal of Peasant Studies 45(1): 192–219. DOI: https://doi.org/10.1080/03066150.2016.1276450

Khan M R, Raja W, Bhat T A, Mir M S, Naikoo N B, Amin Z and Patyal D. 2022. Zero budget natural farming: A way forward towards sustainable agriculture. Current Journal of Applied Science and Technology 41(13): 31–43. DOI: https://doi.org/10.9734/cjast/2022/v41i1331712

Kira O, Linker R and Gitelson A. 2015. Non-destructive estimation of foliar chlorophyll and carotenoid contents: Focus on informative spectral bands. International Journal of Applied Earth Observation and Geoinformation 38: 251–60. DOI: https://doi.org/10.1016/j.jag.2015.01.003

Kizhedath A and Suneetha V. 2011. Estimation of chlorophyll content in common household medicinal leaves and their utilisation to avail health benefits of chlorophyll. Journal of Pharmacy Research 4(5): 1412–13.

Khoo H E, Prasad K N, Kong K W, Jiang Y and Ismail A. 2011. Carotenoids and their isomers: Colour pigments in fruits and vegetables. Molecules 16(2): 1710–38. DOI: https://doi.org/10.3390/molecules16021710

Lakhani H N. 2024. Jeevamrit: Cultivating sustainable agriculture for a resilient and eco-friendly future. International Journal of Current Microbiology and Applied Sciences 13(6): 90–95. DOI: https://doi.org/10.20546/ijcmas.2024.1306.010

Lawson T and Blatt M R. 2014. Stomatal size, speed, and responsiveness impact on photosynthesis and water use efficiency. Plant Physiology 164(4): 1556–70. DOI: https://doi.org/10.1104/pp.114.237107

Mountain Agroecosystem: Status and Scope. Journal of the Indian Society of Soil Science 72(Special issue): I112–I122.

Mukherjee K, Konar A and Ghosh P. 2022. Organic farming in India: A brief review. International Journal of Research in Agronomy 5(2): 113–18. DOI: https://doi.org/10.33545/2618060X.2022.v5.i2b.120

Naguib N Y M, El-Baz F K, El-Baroty G S, Ibrahim M A and Salem W M. 2012. Phenolic, flavonoid and antioxidant activity of some vegetable extracts. Journal of Medicinal Plants Research 6(5): 861–65.

Patel M, Islam S, Glick B R, Choudhary N, Yadav V K, Bagatharia S and Patel A. 2024. Zero budget natural farming components jeevamrit and beejamrit augment Spinacia oleracea L. (spinach) growth by ameliorating the negative impacts of salt and drought stress. Frontiers in Microbiology 15: 1326390. DOI: https://doi.org/10.3389/fmicb.2024.1326390

Rajapriya S, Geetha A and Kripa G K. 2017. A study on the GCMS analysis of bioactive components and pancreato-protective effect of methanolic extract of Brassica oleracea L. var. botrytis. Natural Product Research 31(18): 2174–77. DOI: https://doi.org/10.1080/14786419.2017.1278596

Singh S, Yadav S K, Choudhary M and Tripathi R. 2021. Role of jeevamrit in sustainable agriculture: A review. Journal of Pharmacognosy and Phytochemistry 10(2): 1910–14.

Seong G U, Hwang I W and Chung S K. 2016. Antioxidant capacities and polyphenolics of Chinese cabbage (Brassica rapa L. ssp. pekinensis) leaves. Food Chemistry 199: 612–18. DOI: https://doi.org/10.1016/j.foodchem.2015.12.066

Shah S N, Ahmad M, Zafar M, Ullah F, Zaman W, Mazumdar J, Khuram I and Khan S M. 2019. Leaf micromorphological adaptations of resurrection ferns in northern Pakistan. Flora 255: 1–10. DOI: https://doi.org/10.1016/j.flora.2019.03.018

Singleton V L and Rossi J A. 1965. Colourimetry of total phenolics with phosphomolybdic-phosphotungstic acid reagents. American Journal of Enology and Viticulture 16(3): 144–58. DOI: https://doi.org/10.5344/ajev.1965.16.3.144

Statilko O, Tsiaka T, Sinanoglou V J and Strati I F. 2024. Overview of phytochemical composition of Brassica oleraceae var. capitata cultivars. Foods 13(21): 3395. DOI: https://doi.org/10.3390/foods13213395

Taiz L, Zeiger E, Moller I M and Murphy A. 2015. Plant Physiology and Development, 6th edn. Sinauer Associates, Sunderland, MA, USA.

Vimala P, Illias M K and Salbiah H. 2004. Effect of rates of organic fertiliser on growth, yield and nutrient content of cabbage (Brassica oleracea var. capitata) grown under shelter. Acta Horticulturae 710: 391–98. DOI: https://doi.org/10.17660/ActaHortic.2006.710.47

Watson D J. 1947. Comparative physiological studies on the growth of field crops: I. Variation in net assimilation rate and leaf area DOI: https://doi.org/10.1093/oxfordjournals.aob.a083148

between species and varieties and within and between years. Annals of Botany 11: 41–76.

Williams W, Cuvelier M E and Berset C L W T. 1995. Use of a free radical method to evaluate antioxidant activity. LWT Food Science and Technology 28(1): 25–30. DOI: https://doi.org/10.1016/S0023-6438(95)80008-5

Wang J, Liu Z, Dou J, Lv J, Jin N, Jin L, Li Z, Zhang B, Tang Z and Yu J. 2022. A comparative study on the nutrients, mineral elements and antioxidant compounds in different types of cruciferous vegetables. Agronomy 12(12): 3121. DOI: https://doi.org/10.3390/agronomy12123121

Zhang H and Tsao R. 2016. Dietary polyphenols, oxidative stress and antioxidant and anti-inflammatory effects. Current Opinion in Food Science 8: 33–42. DOI: https://doi.org/10.1016/j.cofs.2016.02.002