Genetic diversity assessment in Indian jute (Corchorus spp) cultivars using  gene-targeted molecular markers

Mukesh Kumar Rana; Sonika Singh; Amit Kumar Singh; Anjali Kak

doi:10.56093/ijas.v82i8.23045

Authors

Mukesh Kumar Rana National Bureau of Plant Genetic Resources, Pusa, New Delhi 110 012
Sonika Singh National Bureau of Plant Genetic Resources, Pusa, New Delhi 110 012
Amit Kumar Singh National Bureau of Plant Genetic Resources, Pusa, New Delhi 110 012
Anjali Kak National Bureau of Plant Genetic Resources, Pusa, New Delhi 110 012

https://doi.org/10.56093/ijas.v82i8.23045

Keywords:

Corchorus spp, Gene-targeted markers, Genetic diversity, Molecular markers

Abstract

During the last two decades a number of molecular marker techniques like RAPD, AFLP, ISSR etc. have been used in crop plants for various applications. With the genomics research gaining momentum, many novel gene-targeted markers which are derived from polymorphic sites within the genes have been developed that can be used for diversity analysis. In the present study, 31 jute cultivars belonging to two cultivated species Corchours olitorius and C. capsularis were evaluated using novel, genomics-information-related technique called Start Codon Targeted (SCoT) markers for the first time. Twenty-two SCoT primers resulted in 67.3% and 62.4% polymorphism in C. olitorius and C. capsularis, respectively. The extent of genetic diversity observed was comparable to earlier findings using other marker techniques. All the cultivars could be unequivocally differentiated from one another. Results on the pattern of diversity observed and the utility of these markers in jute improvement are discussed.

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