Antagonistic effect of Cry1Ac and Cry1Jb on cotton bollworm (Helicoverpa armigera)

ANURADHA CHELLIAH; GORAKH PRASAD GUPTA; SASIKUMAR KARUPPIAH; POLUMETLA ANANDA KUMAR

doi:10.56093/ijas.v82i10.24189

Authors

ANURADHA CHELLIAH National Research Centre on Plant Biotechnology, Pusa, New Delhi 110 012
GORAKH PRASAD GUPTA National Research Centre on Plant Biotechnology, Pusa, New Delhi 110 012
SASIKUMAR KARUPPIAH National Research Centre on Plant Biotechnology, Pusa, New Delhi 110 012
POLUMETLA ANANDA KUMAR National Research Centre on Plant Biotechnology, Pusa, New Delhi 110 012

https://doi.org/10.56093/ijas.v82i10.24189

Keywords:

Bacillus thuringiensis, Cry1Ac, Cry1Jb, Helicoverpa armigera, LC50, Ligand blotting

Abstract

The interaction of two crystal proteins, Cry1Ac and Cry1Jb was evaluated against cotton bollworm (Helicoverpa armigera). The 50% lethal concentrations (LC₅₀s) were estimated to be 2.29 ng/ml and 6 039 ng/ml for Cry1Ac and Cry1Jb, respectively. When mixtures of these toxins in different proportion were assayed, the expected LC₅₀s were lower than the observed LC₅₀s, indicating a clear antagonism between the two toxins. Ligand blotting showed that both the toxins bind to 110kDa APN corroborating the results. This is the first report of antagonism of Cry1Jb with Cry1Ac toxin against cotton bollworm. This trait may prove useful for combating insecticide resistance and for improving the activity of microbial insecticides.

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Author Biographies

ANURADHA CHELLIAH, National Research Centre on Plant Biotechnology, Pusa, New Delhi 110 012
GORAKH PRASAD GUPTA, National Research Centre on Plant Biotechnology, Pusa, New Delhi 110 012
SASIKUMAR KARUPPIAH, National Research Centre on Plant Biotechnology, Pusa, New Delhi 110 012
POLUMETLA ANANDA KUMAR, National Research Centre on Plant Biotechnology, Pusa, New Delhi 110 012

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