Shelf-life of Trichoderma viride in talc and charcoal based formulations

Abstract

The shelf-life of Trichoderma viride was studied in talc and charcoal based formulations. Potato dextrose broth talc based formulation was prepared by adding three different volumes of biomass of T. viride along with medium @ 30, 40 and 50 ml/g talc. The initial mean CFU of T. viride at 30, 40 and 50 ml/100 g talc on 0 day was 227.2 × 10⁹, 256.00 × 10⁹ and 291.03 × 10⁹ CFU/g sample, respectively. It gradually declined and at 120 days of storage the population came down to 70.33 × 10⁹, 80.67 × 10⁹ and 96.67 × 10⁹ CFU/g which in terms of roeduction in viability were 69.04, 68.48 and 66.78% respectively. Similar trends of results were recorded in all the three sorghum grains charcoal based formulations almost at par with each other ranging from 71.14 to 72.44% in 120 days. Sufficient numbers of spores were viable even after 120 days of storage in the formulation indicating that the formulation can be stored further for more time.