Effect of total protein, total phenol, polyphenoloxidase, and peroxidase activity in rice (Oryza sativa) genotypes against Magnaporthe grisea

P D THONGBAM; AMIT KUMAR; KIT KUPAR LYNDOH NONGLAIT; AVINASH PANDEY

doi:10.56093/ijas.v84i2.38031

Authors

P D THONGBAM ICAR RC for NEH Region, Umiam, Meghalaya 793 103
AMIT KUMAR ICAR RC for NEH Region, Umiam, Meghalaya 793 103
KIT KUPAR LYNDOH NONGLAIT ICAR RC for NEH Region, Umiam, Meghalaya 793 103
AVINASH PANDEY ICAR RC for NEH Region, Umiam, Meghalaya 793 103

https://doi.org/10.56093/ijas.v84i2.38031

Keywords:

Peroxidase, Polyphenoloxidase, Repeated measure analysis, Rice blast, Total phenol, Total protein

Abstract

The objective of our study was to know the differences in expression of total protein, total phenol, peroxidase and polyphenoloxidase activities in self-defense in plant tissues against rice blast diseases. The activities of these compounds were studied in different genotypes of rice (Oryza sativa L.) Ngoba, Bhalum-1, Lampnah and IET 7614 with varying degrees of susceptibility to Magnaporthe grisea. Observation was taken after two successive days interval up to 15 days repeatedly (eight times) and the data was subjected to repeated measure analysis. Gradual declination was observed for total protein, whereas total phenol, peroxidase and polyphenoloxidase activity has shown increasing trend. Rice genotypes have shown considerable difference in expression which was well-correlated with the degrees of resistance in specificity of pathogen. Univariate test for between and within subject factors were calculated and found to be significant (P<0.001). Since sphericity assumption was violated, MANOVA test was also done and significant difference among characters and genotypes were observed in due course of time.

Downloads

Download data is not yet available.

References

Babujee L and Gnanamanickam S S. 2000. Molecular tools for characterization of rice blast pathogen (Magnaporthe grisea) population and molecular marker-assisted breeding for disease resistance. Current Science 78(3): 248–57.

Doke N. 1983. Involvement of superoxide anion generation in the hypersensitive response of potato tuber tissues to infection with an incompatible race of Phytophthora infestans and to the hyphal wall components. Physiological Plant Pathology 23: 345–57. DOI: https://doi.org/10.1016/0048-4059(83)90019-X

Eyduran E and Akbaþ Y. 2010. Comparison of different covariance structure used for experimental design with repeated measurement. Journal of Animal and Plant Sciences 20(1): 44– 51.

Farkas GL, Dezsi L, Horvath M, Kisban K and Udvardy J. 1963. Common pattern of enzymatic changes in detached leaves and tissues attacked by parasites. journal of phytopathology- phytopathologische zeitschrift 49: 343–54.

Gurbuz F, Baþpýnar E, Çamdeviren H and Keskin S. 2003. Analyzing experimental designs with repeated measurement. Publishing No. 130, Yuzuncu Yil University, Van.

Kar M and Mishra D. 1976. Catalase, peroxidase, and polyphenoloxidase activities during rice leaf senescence. Plant Physiology 57: 315–19. DOI: https://doi.org/10.1104/pp.57.2.315

Lamb C and Dixon R A. 1997. The oxidative burst in plant disease resistance. Annual Review of Plant Physiology and Molecular Biology of Plants 48: 251–75. DOI: https://doi.org/10.1146/annurev.arplant.48.1.251

Littell R C, Henry P R and Ammerman C B. 1998. Statistical analysis of repeated measures data using SAS procedures. Journal of Animal Science 76(4): 1 216–31. DOI: https://doi.org/10.2527/1998.7641216x

Littell R C, Milliken G A, Stroup W W and Wolfinger R D. 1996. SAS System for Mixed Models, SAS Institute Inc (Data Set 6.4). Cary, NC: http://support.sas.com/

Lowry O H, Rosebrough N J, Farr A L and Randall R J. 1951. Protein measurement with the Folin phenol reagent. Journal of Biological Chemistry 193: 265–75. DOI: https://doi.org/10.1016/S0021-9258(19)52451-6

Maraite H. 1973. Changes in polyphenol oxidases and peroxidases in muskmelon (Cucumis melo L.) infected by Fusarium oxysporum f. sp. melonis. Physiological Plant Pathology. 3: 29– 49. DOI: https://doi.org/10.1016/0048-4059(73)90020-9

Peng M and Kuc J. 1992. Peroxidase-generated hydrogen peroxide as a source of antifungal activity in vitro and on tobacco leaf disks. Phytopathology 82: 696–9. DOI: https://doi.org/10.1094/Phyto-82-696

Rodrigues F A, Benhamou N, Datnoff L E, Jones J B and Belanger R R. 2003. Ultrastructural and cytochemical aspects of silicon- mediated rice blast resistance. Phytopathology 93(5): 535–46. DOI: https://doi.org/10.1094/PHYTO.2003.93.5.535

Sasaki K, Iwai T , Hiraga S, Kuroda K, Seo S, Mitsuhara I, Miyasaka A, Iwano M, Ito H, Matsui H and Ohashi Y. 2004. Ten rice peroxidases redundantly respond to multiple stresses including infection with rice blast fungus. Plant Cell Physiology 45(10): 1442–52. DOI: https://doi.org/10.1093/pcp/pch165

Soanes D M, Chakrabarti A, Paszkiewicz K H, Dawe A L and Talbot NJ. 2012. Genome-wide transcriptional profiling of appressorium development by the rice blast fungus magnaporthe oryzae. PLOS Pathogens 8(2): e1002514. doi:10.1371/journal.ppat.1002514. DOI: https://doi.org/10.1371/journal.ppat.1002514

Sridhar R and Ou S H. 1974. Biochemical changes associated with the development of resistant and susceptible types of rice blast lesions. Phytopathology 79: 222–30. DOI: https://doi.org/10.1111/j.1439-0434.1974.tb02704.x

Sridhar R. 1972. Influence of nitrogen fertilization and Pyricularia oryzae development on some oxidases, their substrates and respiration of rice plant. Acta Phytopathologica Academiae Scientiarum Hungaricae 7: 57–70.

Tabachnick B G and Fidel L S. 2001. Using Multivariate Statistics. Allyn and Bacon, USA.

Tanabe S, Minami N I, Saitoh K I, Otake Y, Kaku H, Shibuya N, Nishizawa Y and Minami E. 2011. The role of catalase-peroxidase secreted by Magnaporthe oryzae during early infection of rice cells. Molecular Plant-Microbe Interactions 24(2): 163–71. DOI: https://doi.org/10.1094/MPMI-07-10-0175

Toyoda S and Suzuki N. 1960. Histochemical studies on rice blast lesions. IV. Changes in the activity of oxidases in infected tissue. Annals of the Phytopathologicial Society of Japan 25: 172–77. DOI: https://doi.org/10.3186/jjphytopath.25.172

Yun SC, Kim PG and Park EW. 2000. Alteration of gas exchange in rice leaves infected with Magnaporthe grisea. Plant Pathology Journal 16(5): 279–85.