Phosphate solubilising Streptomyces spp obtained from the rhizosphere of Ceriops decandra of Corangi mangroves

D V SUBHASHINI; ANIL KUMAR V

doi:10.56093/ijas.v84i5.40467

Authors

D V SUBHASHINI Central Tobacco Research Institute, Rajahmundry, Andhra Pradesh 533 105
ANIL KUMAR V Central Tobacco Research Institute, Rajahmundry, Andhra Pradesh 533 105

DOI:

https://doi.org/10.56093/ijas.v84i5.40467

Keywords:

Indole-3-acetic acid, Mangrove, Phosphate-solubilizing microorganisms, Rhizosphere, Streptomyces

Abstract

Phosphate-solubilizing potential of Streptomyces spp isolated from the rhizosphere soil of Ceriops decandra (Griffth) Ding Hou mangrove plant was studied using culture media supplemented with insoluble tricalcium phosphate which became transparent after few days of incubation. Fifteen phosphate-solubilizing strains of Streptomyces St-1 to St-15 were isolated from the rhizosphere of mangroves during 2010-11. Phosphate-solubilizing activity of these isolates was evaluated by the formation of halos (clear zones) around the colonies growing on solid medium containing tribasic calcium phosphate as a sole phosphorus source. St-3 was the most active phosphate solubilizing strain among the isolates followed by St-11. Studies on the biomass production of St-3 and St-11 showed an increase in growth at pH 7.2 after 7, 15 and 21 days of incubation at temperature 30°C and 37°C. Culture filtrate of St-3 alone showed the production of IAA. Present study reveals that the production of organic acids by the mangrove rhizosphere microorganisms may be a possible mechanism involved in the solubilization of insoluble calcium phosphate.

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References

Ahmad F, Ahmad I and Khan M S. 2005. Indole acetic acid production by the indigenous isolates of Azotobacter and fluorescent Pseudomonas in the presence and absence of tryptophan. Turkish Journal of Biology 29: 29–34.

Bhargava B S and Raghupathi H B. 1993. Analysis of plant materials for macro and micronutrients. (In) Methods of Analysis of Soil, Plants, Waters and Fertilizers, pp 49–82. Tandon HLST (Ed). Fertilizer Development and Consultation Organization, New Delhi.

Bano N and Musarrat J. 2003. Characterization of a new Pseudomonas aeruginosa strain NJ-15 as a potential biocontrol agent. Current Microbiology 46: 324–8. DOI: https://doi.org/10.1007/s00284-002-3857-8

Berdy J. 2005. Bioactive microbial metabolites. A personal view. Journal of Antibiotics 58: 1–26. DOI: https://doi.org/10.1038/ja.2005.1

Dave A and Patel H H. 2003. Impact of different carbon and nitrogen sources on phosphate solubilization by Pseudomonas fluorescens. Indian Journal of Microbiology 43: 33–6.

Gupta N, Sahoo Dolly and Basak Uday Chand. 2010. Evaluation of in vitro solubilization potential of phosphate solubilising Streptomyces isolated from phyllosphere of Heritiera fomes (mangrove). African Journal of Microbiology Research 4(3) : 136–42.

Khamna S, Akira Yokota, John F Peberdy and Sisamorn Lumyong. 2010. Indole-3-acetic acid production by Streptomyces spp isolated from some Thai medicinal plant rhizosphere soils. European Asian Journal of Biosciences 4 : 23–32. DOI: https://doi.org/10.5053/ejobios.2010.4.0.4

Kundu B S, Gera R, Sharma N, Bhatia A and Sharma R. 2002. Host specificity of phosphate solubilising bacteria. Indian Journal of Microbiology 42 : 19–21.

Matsukawa E, Nakagawa Y, Limura Y and Hayakawa M. 2007. Stimulatory effect of indole-3-acetic acid on aerial mycelium formation and antibiotic production in Streptomyces spp. Actinomycetologica 21 : 32–9. DOI: https://doi.org/10.3209/saj.SAJ210105

Narayana K J P, Prabhakar P, Vijayalakshmi M, Venkateswarlu Y and Krishna P S J. 2007. Biological activity of phenylpropionic acid from a terrestrial Streptomycetes. Polish Journal of Microbiology 56 : 191–7.

Selvam V, Karunagaran V M. 2004. Coastal wetlands: Mangrove conservation and management. (In) Orientation guide 1. Ecology and Biology of Mangroves. M S Swaminathan Research Foundation, Chennai.

Sinha B. 2012. Global biopesticide research trends: a bibliometric assessment. Indian Journal of Agricultural Sciences 82(2) : 95–101.

Srivastav S, Yadav K S and Kundu B S. 2004. Prospects of using phosphate solubilizing Pseudomonas as biofungicide. Indian Journal of Microbiology 44: 91–4.

Subhashini D V. 2010. Biological control of damping-off (Pythium aphanidermatum) using Streptomyces lavendulae isolate 21 on tobacco seedbeds. Journal of Biological Control 24(4): 338– 42.

Subhashini D V and Padmaja K. 2011. Population dynamics and screening of phosphate-solubilizing bacteria isolated from tobacco (Nicotiana tabacum) – based cropping systems. Indian Journal of Agricultural Sciences 81(8): 740–3.

Vazquez P G, Holguin M E, Puente A, Cortes L and Bashan Y. 2000. Phosphate-solubilising microorganisms associated with the rhizosphere of mangroves in a semiarid coastal lagoon. Biology and Fertility of Soils 30: 460–8. DOI: https://doi.org/10.1007/s003740050024