Gamma radiation induced variations in corn marigold (Glebionis segetum) and their RAPD-based genetic relationship

MANISH KAPOOR; PRABHAT KUMAR; SHANT LAL

doi:10.56093/ijas.v84i7.41930

Authors

MANISH KAPOOR Punjabi University, Patiala, Punjab 147 002
PRABHAT KUMAR Indian Agricultural Research Institute, New Delhi
SHANT LAL College of Agriculture, G B Pant University of Agriculture and Technology, Pantnagar, Uttarakhand 263 145

https://doi.org/10.56093/ijas.v84i7.41930

Keywords:

Gamma rays, Glebionis segetum, Irradiation, Mutation, Mutants, RAPD

Abstract

The present investigation was conducted during the kharif season of 2010-2012 to study hormesis, morphological and biochemical attributes associated with mutation and purification of novel types in corn marigold. The seeds of Glebionis segetum were treated with gamma rays (Source ⁶⁰Co) at the dose of 20, 40, 60, 80 and 100 Gy were sown in the field along with control (un-irradiated seeds). Low doses of gamma irradiation resulted in hormesis and induced encouraging novelties, while the higher doses induced higher degree of abnormalities which led to mortality. The M₂ seeds were sown to observe their characters and mutants in each population. Five promising mutants, viz. S₁-S₃ at 20 Gy and S₄-S₅ at 40 Gy gamma irradiation treatments were tagged, screened and checked for stability of characters for genetic study and possible uses of the traits. RAPD molecular marker technique was used for the study of genetic divergence and establishment of distinctiveness or similarity between the mutants developed as a result of mutagenic treatment.

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References

Banerji B K and Datta S K. 1992. Gamma rays induced flower shape mutation in Chrysanthemum cv ‘Jaya’. Journal of Nuclear Agriculture and Biology 21: 73–9.

Banerji B K and Datta S K. 2001. Induction and analysis of somatic mutation in Chrysanthemum cv.‘Surekha’. Journal of Nuclear Agriculture and Biology 30: 83–90.

Banerji B K and Datta S K. 2002. Induction and analysis of gamma ray-induced flower head shape mutation in ‘Lalima’ Chrysanthemum (Glebionis morifolium). Indian Journal of Agricultural Sciences 72: 6–10.

Broertjes C. 1968. Mutation breeding of vegetatively propagated crops. (In) Mutation in Plant Breeding II. Proc. FAO/IAEA, Milan, Italy, pp 139–65.

Datta S K. 1984. Radiosensitivity. Everyman’s Science 19: 269–73.

Datta S K. 1988. ‘Agnishikha’ - A new Chrysanthemum cultivar evolved by gamma irradiation. Floriculture 9: 10.

Dilta B S, Sharma Y D, Gupta Y C, Bhalla, R and Sharma B P. 2003. Effect of gamma rays on vegetative and flowering parameters of Chrysanthemum. Journal of Ornamental Horticulture 6: 328–34.

Doyle J J and Doyle J L. 1990. Isolation of plant DNA from fresh tissues. Focus 12: 13–5.

Gaul H. 1970. Plant injury and lethality. (In) Manual on Mutation Breeding. Vienna, FAO/IAEA, pp 85–90.

Gomez K A and Gomez A A. 1984. Statistical Procedures for Agricultural Research, 2nd ed. John Wiley and Sons, New York.

Gordon S A. 1957. The effect of ionizing radiation on plants: biochemical and physiological aspects. Quarterly Review Biology 32: 3–14. DOI: https://doi.org/10.1086/401669

Gunckel J E and Sparrow A H. 1961. Ionizing radiations:biochemical, physiological aspects of their effects on plants. Encyclopedia of Plant Physiology 16: 555–617.

Hiscox J D and Israelstam G F. 1979. A method for the extraction of chlorophyll from leaf tissue without maceration. Canadian Journal of Botany 57: 1 332–4. DOI: https://doi.org/10.1139/b79-163

Jaccard P. 1908. Nouvelles recherches sur la distribution florale. Bulletin of Society Vaudoise Science and Nature 44: 223.

Jain H K and Mazumdar P K. 1957. Some cytological and phenotypic effects of x-irradiation in annual Chrysanthemum. Indian Journal of Horticulture 14: 131–40.

Jain H K, Bose A K, Sathpathy D and Sur S C. 1961. Mutation studies in annual Chrysanthemum. I. Radiation induced variation in flower form. Indian Journal of Genetics and Plant Breeding 21: 68–74.

Lamseejan S, Jompuk P and Deeseepan S. 2003. Improvement of Chrysanthemum variety ‘Taihei’ through in vitro induced mutation with chronic and acute gamma rays. Journal of Nuclear Society (Thailand). 4: 2–13.

Mahure H R, Choudhry M L, Prasad K V and Singh S K. 2010. Mutation in Chrysanthemum through gamma irradiation. Indian Jounal of Horticulture 67: 356–8.

Misra P, Banerji B K and Kumari A. 2009. Effect of gamma irradiation on Chrysanthemum cultivar ‘Pooja’ with particular reference to induction of somatic mutation in flower colour and form. Jounal of Ornamental Horticulture 12: 213–6.

Rana R S. 1965. Radiation induced variation in ray floret characteristic of annual Chrysanthemum. Euphytica 14: 296– 300. DOI: https://doi.org/10.1007/BF00149515

Riek D J. 2001. Are molecular markers strengthening plant variety registration and protection? (In) Proc XX EUCARPIA Symposium Strategies foe New Ornamentals. Part I. Van Huylenbroeck J, Van Bockstaele E, Debergh P (Eds). Acta Horticulture 552: 215–23. DOI: https://doi.org/10.17660/ActaHortic.2001.552.24

Rohlf F J. 2009. NTSYSpc: Numerical Taxonomy and Multivariate Analysis Systems. Version 2.2E. Exeter Publishing Ltd, Setauket, New York.

Schum A. 2003. Mutation breeding in ornamentals: an efficient breeding method. Acta Horticulture 612: 47–60. DOI: https://doi.org/10.17660/ActaHortic.2003.612.6

William J G, Kubelik A R, Livak K J and Raafalski J A. 1990. DNA polymorphisms amplified by arbitrary primers are useful as genetic markers. Nucleic Acids Research 18: 6 531–5. DOI: https://doi.org/10.1093/nar/18.22.6531

Zargar G H, Singh H C P, Zeerak N A, Wani S A and John A Q. 1998. Gamma ray induced somatic mutations in Chrysanthemum. (In) Exploring the Chrysanthemum in India. Proceedings of National Conference on Chrysanthemum, 4-5 December 1997, NBRI, Lucknow, India, pp 105–10.