Influence of different carbon sources on in vitro induction of anthocyanin pigments in callus cultures of petunia (Petunia hybrida)

USHA; T JANAKIRAM; K V PRASAD; CHARANJIT KAUR; P SRINIVASAN; C TARA SATYAVATHI

doi:10.56093/ijas.v84i8.43085

Authors

USHA Indian Agricultural Research Institute, New Delhi 110 012
T JANAKIRAM Indian Agricultural Research Institute, New Delhi 110 012
K V PRASAD Indian Agricultural Research Institute, New Delhi 110 012
CHARANJIT KAUR Indian Agricultural Research Institute, New Delhi 110 012
P SRINIVASAN Indian Agricultural Research Institute, New Delhi 110 012
C TARA SATYAVATHI Indian Agricultural Research Institute, New Delhi 110 012

https://doi.org/10.56093/ijas.v84i8.43085

Keywords:

Anthocyanin, Callus cultures, Colour value index, Fructose, Galactose, Glucose, Petunia hybrida, Sucrose

Abstract

Anthocyanins are naturally occurring compounds that impart color to fruits, flowers, vegetables, and plants. They are probably the most important group of visible plant pigments besides chlorophyll pigments. Apart from imparting color to plants, anthocyanins also have an array of health-promoting benefits, as they can protect against a variety of free radicals through a various number of mechanisms. Development of an efficient tissue culture system for commercial production of anthocyanins requires an integrated approach through manipulation of various media constituents. The effect of varied concentrations of different carbon sources on anthocyanin production in callus cultures of Petunia hybrida cv Bravo Blue was studied. Explants from young leaves were cultured on Murashige and Skoog (MS) medium supplemented with MS + IBA (19.6 µM) + Kin. (4.65 µM) + AdS (81.45 mM), 3% sucrose and 0.7% agar. Among the various carbon sources tested, incorporation of Glucose at 5% was found to have earliest pigment induction with maximum response coefficient with highest pigment content (1.36 ± 0.012 CV/g FCW). Highest gain in fresh cell weight was noticed with the addition of sucrose 5% (3.96 ± 0.06 g). When MS medium was supplemented with different concentrations of Galactose, the explants failed to respond.

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