Influence of different carbon sources on in vitro induction of anthocyanin pigments in callus cultures of petunia (Petunia hybrida)


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Authors

  • USHA Indian Agricultural Research Institute, New Delhi 110 012
  • T JANAKIRAM Indian Agricultural Research Institute, New Delhi 110 012
  • K V PRASAD Indian Agricultural Research Institute, New Delhi 110 012
  • CHARANJIT KAUR Indian Agricultural Research Institute, New Delhi 110 012
  • P SRINIVASAN Indian Agricultural Research Institute, New Delhi 110 012
  • C TARA SATYAVATHI Indian Agricultural Research Institute, New Delhi 110 012

https://doi.org/10.56093/ijas.v84i8.43085

Keywords:

Anthocyanin, Callus cultures, Colour value index, Fructose, Galactose, Glucose, Petunia hybrida, Sucrose

Abstract

Anthocyanins are naturally occurring compounds that impart color to fruits, flowers, vegetables, and plants. They are probably the most important group of visible plant pigments besides chlorophyll pigments. Apart from imparting color to plants, anthocyanins also have an array of health-promoting benefits, as they can protect against a variety of free radicals through a various number of mechanisms. Development of an efficient tissue culture system for commercial production of anthocyanins requires an integrated approach through manipulation of various media constituents. The effect of varied concentrations of different carbon sources on anthocyanin production in callus cultures of Petunia hybrida cv Bravo Blue was studied. Explants from young leaves were cultured on Murashige and Skoog (MS) medium supplemented with MS + IBA (19.6 µM) + Kin. (4.65 µM) + AdS (81.45 mM), 3% sucrose and 0.7% agar. Among the various carbon sources tested, incorporation of Glucose at 5% was found to have earliest pigment induction with maximum response coefficient with highest pigment content (1.36 ± 0.012 CV/g FCW). Highest gain in fresh cell weight was noticed with the addition of sucrose 5% (3.96 ± 0.06 g). When MS medium was supplemented with different concentrations of Galactose, the explants failed to respond.

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References

Cordts J, Scorza R and Bell R L. 1987. Effects of carbohydrates and nitrogen on the development of anthocyanins of a red leaf peach (Prunus persica L. Batsch) in vitro. Plant Cell Tissue Organ Culture 9: 103–10. DOI: https://doi.org/10.1007/BF00044245

Do C B and Cormie F. 1990. Accumulation of anthocyanins enhanced by a high osmotic potential in grape (Vitis vinifera L.) cell suspensions. Plant Cell Reports 9: 143–6. DOI: https://doi.org/10.1007/BF00232091

Dornenburg H and Knorr D. 1996. Generation of colors and flavors in plant cell and tissue cultures. Critical Reviews in Plant Sciences 15: 141–68. DOI: https://doi.org/10.1080/07352689.1996.10393184

Jang J C, Leon P, Zhou L and Sheen J. 1997. Hexokinase as sugar sensor in higher plants. Plant Cell 9: 5–19. DOI: https://doi.org/10.1105/tpc.9.1.5

Larronde F, Krisa S, Decendit A, CheÁ ze C, De eux G and MeÂrillon J M. 1998. Plant Cell Reports 17: 946. DOI: https://doi.org/10.1007/s002990050515

Lila MA. 2004. Anthocyanins and human health: an in vitro investi- gative approach. Journal of Biomed Biotechnol 5: 306–13. DOI: https://doi.org/10.1155/S111072430440401X

Mederos S and Enriquez M J. 1987. In vitro propagation of golden times roses, factors affecting shoot tips and axillary bud growth and morphogenesis. Acta Horticulturae 212: 619–24. DOI: https://doi.org/10.17660/ActaHortic.1987.212.103

Misawa M. 1994. Plant Tissue Culture: an alternative for production of useful metabolites. Agriculture Services Bull., FAO, Rome, p 87.

Mori T and Sakurai M. 1994. Production of anthocyanin from strawberry cell suspension cultures; effects of sugar and nitrogen. Journal of Food Science 59: 588–93. DOI: https://doi.org/10.1111/j.1365-2621.1994.tb05569.x

Nagarajan R P, Keshavarz E and Gerson D F. 1989. Optimization of anthocyanin yield in a mutated carrot cell line (Daucus carota) and its implications in large scale production. J. Ferm. Bioeng 68: 102–6. DOI: https://doi.org/10.1016/0922-338X(89)90056-1

Rajendran L, Ravishankar G A, Venkataraman L V and Pratibha K R. 1992. Anthocyanin production in callus cultures of Daucus carota as influenced by nutrient stress and osmoticum. Biotechnology Letters 14: 707–12. DOI: https://doi.org/10.1007/BF01021647

Ram M. 2012. ‘Induction and characterization of anthocyanin pigments from callus cultures of rose (Rosa hybrida L.).’ Ph D thesis, Indian Agricultural Research Institute, New Delhi.

Ram M, Prasad K V, Kaur C, Singh S K, Arora A and Kumar S. 2011. Induction of anthocyanin pigments in callus cultures of Rosa hybrida L. in response to sucrose and ammoniacal nitrogen levels. Plant Cell Tissue Organ Culture 104: 171–9. doi: 10.1007/s11240-010-9814-5 DOI: https://doi.org/10.1007/s11240-010-9814-5

Simoes C, Bizarri C H B, da Silva Cordeiro L, de Castro T C, Coutada L C M, da Silva A G R, Albarello N and Mansur E. 2009. Anthocyanin production in callus cultures of Cleome rosea: Modulation by culture conditions and characterization of pigments by means of HPLC-DAD/ESIMS. Plant Physiology and Biochemistry, 47: 895–903. DOI: https://doi.org/10.1016/j.plaphy.2009.06.005

Suzuki M. 1995. Enhancement of anthocyanin accumulation by high osmotic stress and low ph in grape cells (Vitis hybrids). Journal of Plant Physiology 147: 152–5. DOI: https://doi.org/10.1016/S0176-1617(11)81428-8

Tsukaya H, Ohshima T, Naito S, Chino M, and Komeda Y. 1991. Sugar-dependent expression of the CHS-A gene for chalcone synthase from Petunia in transgenic Arabidopsis. Plant Physiology 97: 1 414–21. DOI: https://doi.org/10.1104/pp.97.4.1414

X Vitrac, F Larronde, S Krisa, A Decendit, G Deffieux and J M Me Rillon. 2000. Sugar sensing and Ca2þ-calmodulin requirement in Vitis vinifera cells producing anthocyanins. Phytochemistry 53: 659–65. DOI: https://doi.org/10.1016/S0031-9422(99)00620-2

Zhang W and Furusaki S. 1999. Production of anthocyanins by plant cell cultures. Biotechnology and Bioprocess Engineering 4: 231–52. DOI: https://doi.org/10.1007/BF02933747

Zhou S, Sauve R J and Howard E F. 2002. Identification of a cell wall peroxidase in red calli of Prunus incisa Thunb. Plant Cell Report 21: 380–4. DOI: https://doi.org/10.1007/s00299-002-0527-z

Zwayyed S K, Frazier G C and Dougall D K. 1991. Growth and anthocyanin accumulation in carrot cell suspension cultures growing on fructose, glucose, or their mixtures. Bio-technology Progress 7: 288–90. DOI: https://doi.org/10.1021/bp00009a014

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2014-08-07

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2014-08-08

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USHA, JANAKIRAM, T., PRASAD, K. V., KAUR, C., SRINIVASAN, P., & SATYAVATHI, C. T. (2014). Influence of different carbon sources on in vitro induction of anthocyanin pigments in callus cultures of petunia (Petunia hybrida). The Indian Journal of Agricultural Sciences, 84(8), 957–61. https://doi.org/10.56093/ijas.v84i8.43085
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