Effect of seed moisture content and storage temperature on seed longevity of hemp (Cannabis sativa)


798 / 241

Authors

  • S S PARIHAR Indian Agricultural Research Institute, New Delhi 110 012
  • M DADLANI Indian Agricultural Research Institute, New Delhi 110 012
  • S K LAL Indian Agricultural Research Institute, New Delhi 110 012
  • V A TONAPI Indian Agricultural Research Institute, New Delhi 110 012
  • P C NAUTIYAL Indian Agricultural Research Institute, New Delhi 110 012
  • SUDIPTA BASU Indian Agricultural Research Institute, New Delhi 110 012

https://doi.org/10.56093/ijas.v84i11.44551

Keywords:

Cannabis sativa, Hemp, Orthodox seeds, Storage behaviour

Abstract

Hemp (Cannabis sativa L.) is one of the earliest domesticated plants grown for its protein and oil rich seed, fiber and psychoactive substances and it is one of the earliest known medicinal plants in human history. Studies were conducted on seed germinability (germination test) and viability (topographical tetrazoliun chloride test) in three seed lots to determine the seed quality. Studies conducted on effect of five seed moisture contents (5, 7, 8, 10 and 12 % on fresh weight basis), three storage temperature (ambient, 15°C and -20°C) and eight storage periods (0, 3, 6, 9, 12, 18, 24 and 36 months) on seed longevity revealed that the critical moisture content (moisture content required in seeds for retaining initial germination after storage of seeds up to 36 months) of seeds for ambient storage condition of Delhi was 5 %, which increased to 7 % in 15°C and 12 % at -20°C storage temperature. The seeds are desiccation as well as chilling tolerant, therefore, exhibit orthodox storage behavior and are ideal for ex-situ conservation of seeds in seed/gene banks.

Downloads

Download data is not yet available.

References

Ali N, Probert R, Hay F, Davies H, Stuppy W. 2007. Post-dispersal embryo growth and acquisition of desiccation tolerance in Anemone nemorosa L. seeds. Seed Science Research 17: 155– 63. DOI: https://doi.org/10.1017/S0960258507783149

AOAC. 2005. Official Methods of Analysis, 15th ed. AOSA Inc, USA.

Baskin J M and Baskin C C. 2004. A classification system for seed dormancy. Seed Science Research 14: 1–16. DOI: https://doi.org/10.1079/SSR2003150

Buitink J, Leprince O, Hemminga M A and Hoekstra F A. 2000. Molecular mobility in the cytoplasm: a new approach to describe and predict lifespan of dry germplasm. Proceedings of the National Academy of Sciences of the USA 97: 2385–90. DOI: https://doi.org/10.1073/pnas.040554797

Buitink J and Hoekstra F A. 2004. Understanding and predicting optimal storage condition and longevity. Seed Conservation: Turning Science into Practice, pp 747–59. Smith R D et al. (Eds.) The Royal Botanic Gardens, Kew, London.

Courtwright D T. 2001. Forces of Habit: Drugs and the Making of Modern World. Harverd University Press. DOI: https://doi.org/10.4159/9780674029903

DFSC. 1999. Desiccation and storage protocols. Newsletter No.5, Danida Forest Seed Centre, Humleback, Denmark.

Ellis R H and Roberts E H. 1980. Improved equations for the prediction of seed longevity. Annals of Botany 45: 13–30. DOI: https://doi.org/10.1093/oxfordjournals.aob.a085797

Ellis R H. 1998. Longevity of seeds stored hermetically at low moisture content. Seed Science Research 8 (Supplement No. 1): 9–10.

Ellis R H and Hong T D. 2007. Seed longevity-moisture content relationship in hermetic and open storage. Seed Science & Technology 35: 423–31. DOI: https://doi.org/10.15258/sst.2007.35.2.17

Englemann F and Engels J M M. 2002. Technologies and strategies for ex-situ conservation. Managing Plant Genetic Diversity, pp 89-103. Engles J M M et al. (Eds). BABI Publishing, Wallingford, UK. DOI: https://doi.org/10.1079/9780851995229.0089

Finch-Savage W E and Leubner-Metzer G .2006. Seed dormancy and control of germination. New Phytologist 171: 501–23. DOI: https://doi.org/10.1111/j.1469-8137.2006.01787.x

Forbis T A, Floyd S K and de Queiroz A. 2002. The evolution of embryo size in angiosperms and other seed plants: implications for the evolution of seed dormancy. Evolution 56: 2 112–25 DOI: https://doi.org/10.1554/0014-3820(2002)056[2112:TEOESI]2.0.CO;2

Gold K and Hay F .2008. Equilibrating Seeds to Specific Moisture Levels. Technical information sheet 09, Millennium Seed bank Project, Wakehurst Place, West Sussex, UK.

Harrington J F. 1972. Seed storage longevity. Seed Biology, Volume 3, pp 145–245. Kozlowski T T (Ed), Academic Press, New York. DOI: https://doi.org/10.1016/B978-0-12-395605-7.50009-0

Hong T D and Ellis R H.1996. A Protocol to Determine Seed Storage Behaviour. IPGRI Technical Bulletin No. 1, IPGRI, Rome, pp 1–62.

Ibrahim A E and Roberts E H. 1983. Viability of lettuce seeds. I. Survival in hermetic storage. Journal of Experimental Botany 34:620–30. DOI: https://doi.org/10.1093/jxb/34.5.620

ISTA. 2003. ISTA Working Sheets on Tetrazolium Testing. Vol I Agricultural, Vegetable & Horticultural Species. International Seed Testing Association (ISTA), Bassersdorf, CH-Switzerland. ISTA. 2008. International Rules for Seed Testing, Edition 2008. International Seed Testing Association (ISTA), Bassersdorf, CH-Switzerland.

Lehner A, Corbineau F and Bailly C .2006. Changes in lipid status and glass properties in cotyledons of developing sunflower seeds. Plant Cell Physiology 47:818–28. DOI: https://doi.org/10.1093/pcp/pcj053

Linkies A, Graeber K, Knight C and Leubner-Metzger G .2010. The evolution of seeds. New Phytologist 186: 817-21. DOI: https://doi.org/10.1111/j.1469-8137.2010.03249.x

Liu K, Eastwood R J, Flynn S, Turner R M and Stuppy W H. 2008. Seed information database (release 7.1). http://www.kew.org/data/sid

Nagel M and Borner A. 2010. The longevity of crop seeds stored under ambient condition. Seed Science Research 20: 1–12. DOI: https://doi.org/10.1017/S0960258509990213

Priestley D A, Cullinan V I and Wolfe J . 1985. Differences in seed longevity at the species level. Plant, Cell & Environment 8: 557–62. DOI: https://doi.org/10.1111/j.1365-3040.1985.tb01693.x

Powell A A and Mathews S. 1978. The damaging effect of water on dry pea embryos during imbibition. Journal of Experimental Botany 29: 1 215–29. DOI: https://doi.org/10.1093/jxb/29.5.1215

Downloads

Submitted

2014-11-05

Published

2014-11-12

Issue

Section

Articles

How to Cite

PARIHAR, S. S., DADLANI, M., LAL, S. K., TONAPI, V. A., NAUTIYAL, P. C., & BASU, S. (2014). Effect of seed moisture content and storage temperature on seed longevity of hemp (Cannabis sativa). The Indian Journal of Agricultural Sciences, 84(11), 1303–9. https://doi.org/10.56093/ijas.v84i11.44551
Citation