Genetic diversity of Xanthomonas campestris pv. campestris isolated from Brassica crops using RAPD and Rep-PCR

DINESH SINGH; PRIYANKA SINGH RATHAUR; ASHWARIYA SINGH; RICHA RAGHUWANSHI

doi:10.56093/ijas.v85i6.49230

Authors

DINESH SINGH Principal Scientist (Plant Pathology), Indian Agricultural Research Institute, New Delhi 110 012
PRIYANKA SINGH RATHAUR Senior Research Fellow (Plant Pathology), Division of Pathology, Indian Agricultural Research Institute, New Delhi 110 012
ASHWARIYA SINGH M Sc Student, GLA University Mathura
RICHA RAGHUWANSHI Assistant Professor, Department of Botany, Mahila Maha Vidyalya, Banaras Hindu University, Varanasi, Uttar Pradesh 221 005

https://doi.org/10.56093/ijas.v85i6.49230

Keywords:

Brassica, Crucifers, Genetic diversity, Rep-PCR, RAPD, X. campestris pv. campestris

Abstract

Xanthomonas campestris pv. campestris (Xcc) is causal agent of black rot disease in crucifers. Genetic diversity, 28 isolates of Xcc collected from cole crops (cauliflower, cabbage, broccoli and knol khol), turnip, brown and yellow mustard crops from northern region of India was performed by using rep-PCR fingerprinting (BOX, REP and ERIC) and random amplified polymorphic DNA (RAPD). Five DNA type groups were formed by using rep-PCR fingerprinting. In RAPD study, six primers showed monomorphic bands and remaining six primers (OPB-10, OPB- 15, UBC-245, D-11, UBC-220 and OPA-11) exhibited polymorphic amplified product in all isolates of Xcc. Xcc- C281 and Xcc-C261 and Xcc-C268 were formed separate group as DNA types, 1, 2, 3, 4, 5 and 6 respectively. Genetic variability was found among the isolates of Xcc based on their hosts. They were categorized into eight groups at DNA typing at ~50% similarity coefficient and formed 8 DNA type groups at ~ 50% similarity coefficient. Eighteen isolates of Xcc were grouped under DNA type 7, whereas in DNA type 8 (4 isolates).

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