Effects of gamma irradiation on germination and physiological parameters of maize (Zea mays) genotypes

ACHCHHELAL YADAV; B SINGH; D K SHARMA; S AHUJA

doi:10.56093/ijas.v85i9.51564

Authors

ACHCHHELAL YADAV Scientist, ICAR-Indian Agricultural Research Institute, New Delhi 110 012
B SINGH Principal Scientist, ICAR-Indian Agricultural Research Institute, New Delhi 110 012
D K SHARMA Principal Scientist, ICAR-Indian Agricultural Research Institute, New Delhi 110 012
S AHUJA Senior Research Fellow, ICAR-Indian Agricultural Research Institute, New Delhi 110 012

https://doi.org/10.56093/ijas.v85i9.51564

Keywords:

Alpha amylase activity, Irradiation, Seedling vigor index

Abstract

This study determined the effect of gamma irradiation on the seedling growth parameters and enzymatic activities of gamma irradiated seeds of two maize (Zea mays L.) genotypes. Twelve doses of gamma irradiation ranging from 0.0025 to 2.0 kGy were given to the seeds with the help of ⁶⁰Co γ-radiation source facility available at NRL, IARI, and New Delhi. Water activity, germination per cent, seedling growth, dry weight of seedlings and enzymatic activity were determined by using the standard procedures and protocols. Data obtained showed that the seedling length increased in lower doses (0.0.005 to 0.1 kGy) of gamma irradiation and reduced on and beyond the 0.2 kGy dose for both the genotypes. Similarly seedling vigor index I reduced on and beyond 0.2 kGy of gamma irradiation dose. The germination per cent and alpha amylase activity was significantly more in lower doses (0.0025 to 0.2 kGy) of gamma irradiation and reduced on 0.3 kGy or beyond for both maize genotypes. Water activity of irradiated seeds did not change significantly.

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References

Afrasiab H and Iqbal J. 2012.Genetic analysis of somaclonal variants and induced mutants of potato (Solanum tuberosum L.) cv. Diamant using RAPD markers. Pakistan Journal of Botany 44(1): 215–20.

Bahadori Aliakbar, Mobasser Hamid Reza and Ganjali Hamid Reza .2015. Influence of water stress and plant density on some characteristics in corn. Biological Forum–An International Journal 7(1): 673–8.

Aparna M, Chaturvedi A, Sreedhar M, Pavan Kumar D, Venu- Babu P and Singhal R K. 2013. Impact of gamma rays on the seed germination and seedling parameters of groundnut (Arachis Hypogaea L.). Asian Journal of Experimental Biology 4(1): 61–8.

Borzouei A M, Kafi H, Khazaei B,Naseriyan and Majdabadi A.2010. Effects of gamma radiation germination and physiological aspects of wheat (Triticum aestivum L.) seedlings. Pakistan Journal of Botany 42: 281–90.

Delia Marcu, Grigore Damian, Constantin Cosma.2013. Gamma radiation effects on seed germination, growth and pigment content, and ESR study of induced free radicals in maize (Zea mays). Journal of Biological Physics 39: 625–34 DOI 10.1007/ s10867-013-9322-z DOI: https://doi.org/10.1007/s10867-013-9322-z

Din R, Khan M M, Qasim M, Jehan S and Khan MMI. 2003. Induced mutability studies in three wheat (Triticumaestivum L.) varieties for some morphological and agronomic. Characteristics. Asian Journal of Plant Science 17(2): 1 179– 82. DOI: https://doi.org/10.3923/ajps.2003.1179.1182

DMR. 2014. Annual Report 2013-14, Directorate of Maize Research, Pusa Campus, New Delhi; p 98.

GOI. 2014. Directorate of Economics and statistics. Department of Agriculture and Co-operation, p 86.

Gustavo, Barbosa-Canvas, Anthony, Fontana Jr, Shelly J, Scmidt, Theodore P and Labuza. 2008. Water Activity in foods: Fundamentals and application, 365 p.

Khawar A, Bhatti I A, Khan Q M, Bhatti H N and Sheikh M A. 2010. A germination test: An easy approach to know the irradiation history of seeds. Pakistan Journal of Agricultural Science 47: 279–85.

Kazama Y H, Saito M, Miyagai H, Takehisa H, Ichida Y, Miyazawa K I, Mishiba. 2008. Effect of heavy ion beam irradiation on plant growth and mutation induction in Nicotiana tabacum. Plant Biotechnology 25: 105–11. DOI: https://doi.org/10.5511/plantbiotechnology.25.105

Kiong A, Ling A,Pick S H, Grace Lai and. Harun A R. 2008. Physiological responses of Orthosiphon stamineus plantlets to gamma irradiation. Am-Eurasian Journal of Sustainable . Agriculture 2(2): 135–49.

Kon E, Ahmed O H, Saamin Sand Majid N M. 2007.Gamma Radio sensitivity study on long bean (Vigna sesquipedalis). Americal Journal of Applied Science 4(12): 1 090–3. DOI: https://doi.org/10.3844/ajassp.2007.1090.1093

Maisuria K M and Patel S T. 2009. Seed germinability, root and shoot length and vigour index of soybean as influenced by rhizosphere fungi. Karnataka journal of Agricultural Science 22(5): 1120–2.

Mehlo L M, Bambo Z, Bado S, Lin J, Moasqi S M,Buthelezi S, Stochev B and Chkwamba R .2013. Induced protein polymorphisms and nutritional quality of gamma irradiation mutants of sorghum. Mutation Research 46(1-2): 66–72. DOI: https://doi.org/10.1016/j.mrfmmm.2013.05.002

Maryam L,Yaldagard, Seyed Ali, Mortazavi and FaridehTabatabaie. 2008. Effect of Ultrasonic Power on the activity of barley’s alpha-amylase from post-sowing treate of seeds. World Applied Sciences Journal 3(1): 91–5.

Mongi Melki and Marouni A. 2009. Effects of gamma rays irradiation on seed germination and growth of hard wheat. Environmental Chemistry Lletters 8(4): 307–10. DOI: https://doi.org/10.1007/s10311-009-0222-1

Noreen, Z and M Ashraf. 2009. Changes in antioxidant enzymes and some key metabolites insome genetically diverse cultivars of radish (Raphanus sativus L.). Environmental Experimental Botany 67: 395–402. DOI: https://doi.org/10.1016/j.envexpbot.2009.05.011

Sharma D K and Rana D S. 2007. Response of castor (Ricinus communis L.). Genotypes to low doses of gamma irradiation. Indian Journal of Agricultural Sciences 77(7): 467–9.

Sasikala R and Kalaiyarasi R. 2010. Sensitivity of rice varieties to gamma irradiation. Electronic Journal of Plant Breeding 1(4): 885–9.

Singh B and Datta PS. 2009. Gamma irradiation to improve plant vigour, grain development and yield attributes of wheat. Radiation Physics Chemistry 10: 1 016–22.

Stanley Duncan, Kevin J, Farnden and Elspeth A. 2005.Plant áamylases: functions and roles in carbohydrate metabolism Biologia Bratislava 60/Suppl. 16: 65–71.