SSR based genetic diversity in Abelmoschus species

RANBIR S FOUGAT; ATUR R PUROHIT; SUSHIL KUMAR; MITHIL J PAREKH; MUKESH KUMAR

doi:10.56093/ijas.v85i9.51636

Authors

RANBIR S FOUGAT Professor and Head, Anand Agricultural University, Anand, Gujarat 388 110
ATUR R PUROHIT M Sc Scholar, Anand Agricultural University, Anand, Gujarat 388 110
SUSHIL KUMAR Assistant Professor, Anand Agricultural University, Anand, Gujarat 388 110
MITHIL J PAREKH Ph D Scholar, Anand Agricultural University, Anand, Gujarat 388 110
MUKESH KUMAR Junior Research Fellow, Department of Agricultural Biotechnology, Anand Agricultural University, Anand, Gujarat 388 110

https://doi.org/10.56093/ijas.v85i9.51636

Keywords:

Diversity, Marker, Okra, PCR, SSR

Abstract

Genetic diversity analysis and germplasm characterization are essential steps in plant breeding and molecular markers are proved tool to accomplish. In spite of its high economic value of okra as fresh vegetable in tropical and subtropical regions of Asia and Africa, little attention has been paid to assess its genetic diversity at molecular level. In present study, genetic diversity analysis was performed on 24 Abelmoschus accessions using SSR markers. A total of 85 amplicons were detected from 18 SSR markers. Amplicon analysis revealed a very high level (93.72%) of polymorphism with a mean polymorphic information content (PIC) value of 0.53. Number of alleles scored per primer ranged from 2 to 15 with an average of 4.72 alleles per primer. The UPGMA cluster analysis grouped Abelmoschus genotypes into three main clusters at a cut off value of 0.62. In conclusion, SSR markers enabled discrimination among accessions and provide valuable information for future use in improvement of this genomic resource poor otherwise important vegetable crop.

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