Standardization of protocol for in vitro multiplication of rose (Rosa × hybrida) cv. Happiness

NAMITA NAMITA; D V S RAJU; K V PRASAD; KANWAR P SINGH; SURENDRA KUMAR

doi:10.56093/ijas.v85i11.53763

Authors

NAMITA NAMITA Scientist, Indian Agricultural Research Institute, New Delhi 110 012
D V S RAJU Senior Scientist, Indian Agricultural Research Institute, New Delhi 110 012
K V PRASAD Principal Scientist, Indian Agricultural Research Institute, New Delhi 110 012
KANWAR P SINGH Principal Scientist, Indian Agricultural Research Institute, New Delhi 110 012
SURENDRA KUMAR Senior Technical Officer, Division of Floriculture and Landscaping, Indian Agricultural Research Institute, New Delhi 110 012

https://doi.org/10.56093/ijas.v85i11.53763

Keywords:

Axillary bud, In vitro, Micropropagation, Rosa Ã— hybrida, Regeneration

Abstract

An efficient protocol for in vitro multiplication of Rosa × hybrida L. cv. Happiness was standardized using axillary bud segments. Out of different pre treatments for explants, the highest explant survival (80.25%) was obtained with T₁ pre-treatment comprising 0.2% Carbendazim + 0.2% Mancozeb-45 +150 mg/l 8-HQC for 4 hragitation on a horizontal shaker (200 rpm). Sucrose concentration of 30g/l in the medium was found to be optimum for in vitro shoot multiplication. Murashige and Skoog (MS) medium supplemented with 2.5 mg/l BAP + 5.0 mg/l kinetin + 0.1 mg/l NAA + 0.5 mg/l GA₃ was found most effective for culture establishment, however, MS medium comprising 2.5 mg/l BAP + 2.5 mg/l kinetin + 0.1mg/l NAA+ 0.5mg/l GA₃ along with 40 mg/l adenine sulphate was found to be better for shoot proliferation with highest number of micro shoots (7.10 shoots/explant). Rooting of micro shoots was induced on half strength MS basal medium supplemented with NAA (0.5 mg/l) and IBA (0.5 mg/l) rooting growth regulators. The regenerated plantlets were efficiently hardened in glass jars filled with coco peat + vermiculite + perlite (2:1:1) moistened with half strength MS medium salts and covered with polypropylene lids, thereafter, plants were successfully transferred to the glasshouse with good survival.

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