Morphological and pathogenic variability among Indian populations of Sclerotinia sclerotiorum causing stem rot of chickpea (Cicer arietinum)

A K MANDAL; S C DUBEY

doi:10.56093/ijas.v86i5.58246

Authors

A K MANDAL Bidhan Chandra Krishi Viswavidyalaya, Kalyani, Nadia, West Bengal 741 235
S C DUBEY ICAR-NBPGR, New Delhi 110 012

DOI:

https://doi.org/10.56093/ijas.v86i5.58246

Keywords:

Chickpea, Differentials, Pathotypes, Sclerotinia sclerotiorum, Stem rot, Virulence

Abstract

Stem rot caused by Sclerotinia sclerotiorum (Lib.) de Bary is one of the most important diseases of chickpea (Cicer arietinum L.). Irrespective of hosts of origin, isolates were proved to be pathogenic on chickpea cultivar BDG 1005. Majority of the isolates (20) produced white to dull white and fluffy growth while, only 4 isolates, namely, SS5 (Delhi), SS14 (Uttar Pradesh), SS20 (Haryana) and SS24 (West Bengal) produced suppressed growth without sclerotia which is the rare evidence in this pathogen. Isolates were grouped into three categories on the basis of their growth rate as slow, medium and fast growing. Sclerotia formed in different isolates were highly variable in number as well as in size. Isolate SS6 (Delhi) produced maximum number (50/plate) of smaller size (2.16 mm) sclerotia whereas, isolate SS15 (Himachal Pradesh) produced 21 sclerotia/plate with largest size (4.54 mm). Isolates were also variable in respect of their virulence. They were grouped into 7 pathotypes based on differential reactions on a set of 10 chickpea differential cultivars, namely, DCP 92-3, Pusa 212, Vishal, JG 74, KRW 108, GNG 469, JG 62, GPF 2, Pusa 1073 and WR 315. In the present study, cultivars DCP 92-3, Pusa 212, Vishal, JG 74, GPF 2 and WR 315 were considered to be host differentials for grouping of S. sclerotiorum into 7 pathotypes. Pathotype groups were not followed the continuity with that of geographical origin of isolates. It indicated that each region had mosaic of pathotypes. This is the first study in which the pathotypes of S. sclerotiorum populations were determined.

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References

Allen T D, Dawe A L and Nuss D L. 2003. Use of cDNA microarrays to monitor transcriptional responses of the chestnut blight fungus Cryphonectria parasitica to infection by virulence attenuating hypoviruses. Eukaryotic Cell 2: 1 253–65. DOI: https://doi.org/10.1128/EC.2.6.1253-1265.2003

Asoufi H, Hameed K M and Mahasneh A. 2007. The cellulose and pectinase activities associated with the virulence of indigenous Sclerotinia sclerotiorum isolates in Jordan Valley. Plant Pathology Journal 23: 233–8. DOI: https://doi.org/10.5423/PPJ.2007.23.4.233

Attanyake R, Porter L, Johnson D and Chen W. 2011. Genetic and phenotypic diversity of Sclerotinia sclerotiorum on a small geographic scale. Fungal Genetics Reports 58: 214.

Chen W B, Schatz B, Henson K E, McPhee and Muehlbauer F J. 2006. First report of Sclerotinia stem rot of chickpea caused by Sclerotinia sclerotiorum in North Dakota and Washington. Plant Disease 90: 114. DOI: https://doi.org/10.1094/PD-90-0114A

Fuhlbohm M J, Tatnell J R and Ryley M J. 2003. First report of stem rot and wilt of chickpea caused by Sclerotinia minor in Queensland, Australia. Australasian Plant Pathology 32: 323– 4. DOI: https://doi.org/10.1071/AP03017

Gerwal J S and Pal M. 1986. Fungal disease problems of chickpea. In: Vistas in Plant Pathology, pp 157–70.

Varma A and Verma J P (Eds). MPH, New Delhi, Hambleton S, Walker C and Kohn L M. 2002. Clonal lineages of Sclerotinia sclerotiorum previously known from other crops predominate in 1999-2000 samples from Ontario and Quebec soybean. Canadian Journal of Plant Pathology 24: 309–15. DOI: https://doi.org/10.1080/07060660209507014

Hilton S A. 2000. Canadian plant disease survey. Agriculture and Agri-Food Canada 80: 1–151.

Kim H S, Hartman G L, Manandhar J B, Grief G L, Steadman J R and Diers B W. 2000. Reaction of soybean cultivars to sclerotinia stem rot in field, greenhouse, and laboratory evaluations. Crop Sciences 40: 665–9. DOI: https://doi.org/10.2135/cropsci2000.403665x

Melouk H A, Akem C N and Bowen C. 1992. A detached shoot technique to evaluate the reaction of peanut genotypes to Sclerotinia minor. Peanut Sciences 19: 58–62. DOI: https://doi.org/10.3146/i0095-3679-19-1-16

Sharma S K. 1995. ‘Managemnt of stem rot of gram caused by Sclerotinia sclerotiorum (Lib.) de Bary’. M Sc thesis, Himachal Pradesh Krishi Vishvavidyalaya, Palampur, p 87.

Sun J M, Irzykowski W, Jedryczka M and Han F X. 2005. Analysis of the genetic structure of Sclerotinia sclerotiorum populations from different regions and host plants by random amplified polymorphic DNA markers. Journal of Integrative Plant Biology 47: 385–95. DOI: https://doi.org/10.1111/j.1744-7909.2005.00077.x

Vaid A, Kalha C S, Razdan V K, Tewari A K and Gupta S. 2005. Stem rot of chickpea, its prevalence and management through host resistance in Jammu Division. (In) Abstract book of 1st J&K State Science Congress, p 209.

Xie J, Wei D, Jiang D, Fu Y, Li G, Ghabria S and Peng Y. 2006. Characterization of debilitation-associated mycovirus infecting the plant- pathogenic fungus Sclerotinia sclerotiorum. Journal of General Virology 87: 241–9. DOI: https://doi.org/10.1099/vir.0.81522-0

Xu L, Meichun X, White D and Chen W. 2011. Oxalate-minus mutants of Sclerotinia sclerotiorum via random mutagenesis retain pathogenicity. Phytopathology 101: S104.

Yli-Mattila T, Kalko G, Hannukkala A, Paavanen-Huhtala S and Hakala K. 2010. Prevalence, species composition, genetic variation and pathogenicity of clover rot (Sclerotinia trifolioum) and Fusarium spp. in red colver in Finland. European Journal of Plant Pathology 126: 13–27. DOI: https://doi.org/10.1007/s10658-009-9516-1

Zhang L, Fu Y, Xie J, Jiang D, Li G and Yi X. 2009. A novel virus that infecting hypovirulent strain XG36-1 of plant fungal pathogen Sclerotinia sclerotiorum. Virology Journal 6: 96– 104. DOI: https://doi.org/10.1186/1743-422X-6-96