Microsatellites markers to study genetic relationships among cowpea (Vigna unguiculata) genotypes

HIRA LAL; NAGENDRA RAI; KRISHNA KUMAR RAI; SHAILESH KUMAR TIWARI

doi:10.56093/ijas.v86i5.58351

Authors

HIRA LAL ICAR-Indian Institute of Vegetable Research, PO Jakhini, Shhahanshahpur,Varanasi,Uttar Pradesh 221 305
NAGENDRA RAI ICAR-Indian Institute of Vegetable Research, PO Jakhini, Shhahanshahpur,Varanasi,Uttar Pradesh 221 305
KRISHNA KUMAR RAI ICAR-Indian Institute of Vegetable Research, PO Jakhini, Shhahanshahpur,Varanasi,Uttar Pradesh 221 305
SHAILESH KUMAR TIWARI ICAR-Indian Institute of Vegetable Research, PO Jakhini, Shhahanshahpur,Varanasi,Uttar Pradesh 221 305

DOI:

https://doi.org/10.56093/ijas.v86i5.58351

Keywords:

Cowpea, Genetic diversity, Germplasm management, Microsatellites, Vigna unguiculata

Abstract

Genetic diversity and phylogenetic relationships among 48 cowpea [Vigna unguiculata (L.) Walp.] genotypes collected from different places in India were evaluated using simple sequence repeat molecular markers. A set of 40 primer combinations developed from cowpea genomic/expressed sequence tags and evaluated for their ability to detect polymorphisms among the various cowpea genotypes. Eleven primer combinations detected polymorphisms; sixteen primer combinations were monomorphic, with the remaining 13 primer sets failing to yield PCR amplification products. From one to 5 alleles were found among the informative primer combinations. The genetic diversity of the sample varied from 0.02 to 0.39 (mean = 0.205). The polymorphic information content ranged from 0.12 to 0.86 (mean = 0.49). The inter-cluster representatives of distant clusters (based on morphological and molecular markers) would be more useful for choosing the parents in cowpea breeding programmes.

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