Genetic diversity in Indian bean (Lablab purpureus) accessions as revealed by quantitative traits and cross-species transferable SSR markers

NAGENDRA RAI; SANJEEV KUMAR; RAMESH KUMAR SINGH; KRISHNA KUMAR RAI; GARIMA TIWARI; SARVESH PRATAP KASHYAP; MAJOR SINGH; AWADHESH BAHADUR RAI

doi:10.56093/ijas.v86i9.61518

Authors

NAGENDRA RAI ICAR-Indian Institute of Vegetable Research, Varanasi, Uttar Pradesh 221 305
SANJEEV KUMAR Indian Institute of Sugarcane Research, Dilkusha, Lucknow
RAMESH KUMAR SINGH ICAR-Indian Institute of Vegetable Research, Varanasi, Uttar Pradesh 221 305
KRISHNA KUMAR RAI ICAR-Indian Institute of Vegetable Research, Varanasi, Uttar Pradesh 221 305
GARIMA TIWARI ICAR-Indian Institute of Vegetable Research, Varanasi, Uttar Pradesh 221 305
SARVESH PRATAP KASHYAP ICAR-Indian Institute of Vegetable Research, Varanasi, Uttar Pradesh 221 305
MAJOR SINGH ICAR-Indian Institute of Vegetable Research, Varanasi, Uttar Pradesh 221 305
AWADHESH BAHADUR RAI ICAR-Indian Institute of Vegetable Research, Varanasi, Uttar Pradesh 221 305

https://doi.org/10.56093/ijas.v86i9.61518

Keywords:

Cross-species marker transferability, Diversity, Indian bean, Polymorphism, Quantitative traits, Simple sequence repeats (SSRs)

Abstract

Genetic diversity in 143 Indian bean [Lablab purpureus (L.) Sweet] accessions from India was assessed using simple sequence repeat (SSR) markers. A total of 134 SSR markers developed from common bean, cowpea, mung bean, moth bean and Faba bean were used to assess their transferability to Indian bean. Quantitative traits as well as SSR marker data was used to analyse the genetic diversity analysis in 143 Indian bean accessions from different agroecological zones of India. Of the 134 SSR primers, 55 (41.0%) were found to be transferable since they showed reproducible polymorphic amplification products in Indian bean. The SSR primers derived from common bean fail to amplify any product in Indian bean. The results indicated that there is a potential for transferring SSR markers within legumes. The identification of a set of transferable SSR markers would enable the application of the SSR technology in Indian bean molecular research. To our knowledge, this is the first ever attempt to evaluate cross-species microsatellite (SSR) markers for a possible dissection of genetic diversity in Indian bean.

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