Genetic analysis of bioactive compounds and antioxidant properties in lettuce (Lactuca sativa)

SWATI SAHA; PRITAM KALIA; A K SUREJA; ARPITA SRIVASTAVA; S K SARKAR

doi:10.56093/ijas.v86i11.62931

Authors

SWATI SAHA ICAR-Indian Agricultural Research Institute, Regional Station, Pune, Maharashtra
PRITAM KALIA ICAR-Indian Agricultural Research Institute, Regional Station, Pune, Maharashtra
A K SUREJA ICAR-Indian Agricultural Research Institute, Regional Station, Pune, Maharashtra
ARPITA SRIVASTAVA ICAR-Indian Agricultural Research Institute, Regional Station, Pune, Maharashtra
S K SARKAR ICAR-Indian Agricultural Statistics Research Institute, New Delhi 110 012

https://doi.org/10.56093/ijas.v86i11.62931

Keywords:

Antioxidant, Carotenoids, Chlorophyll, CUPRAC, FRAP, Lactuca sativa, Lettuce, Lycopene, Phenol, Phytochemicals

Abstract

Leaves of lettuce (Lactuca sativa L.) are the store house of various phytonutrients which have protective properties. Being an important dietary leafy vegetable, it is primarily consumed fresh as salad and in sandwiches, burgers etc. Its beneficial effects are primarily due to the presence of different phytochemicals such as ascorbic acid, carotenoids, polyphenols and fibre which helps in protecting key biological constituents such as lipoproteins, membranes and DNA. However, systematic biochemical nutrient analysis has not been carried out in this important salad vegetable so far. In the present investigation, 36 genotypes were analysed for phytochemicals such as total carotenoids, lycopene, ascorbic acid, total phenolic content, Cupric ion Reducing Antioxidant Capacity (CUPRAC) and Ferric Reducing Antioxidant Power (FRAP). The CUPRAC ranged from 0.05 to 1.98 Î¼mol trolox/g with the highest content in Stem lettuce Angustana, whereas FRAP ranged from 0.06 to 4.70 Î¼mol trolox/g showing, thereby, a considerable variation amongst genotypes. Total phenolics ranged from 41.94 to 501.88 Î¼g gallic acid/g fresh weight. Total carotenoids were found in appreciable amount in Wo Suen (46.13 mg/100g fresh weight), whereas lycopene in New Chicken (17.01 mg/100g fresh weight). Ascorbic content ranged from 1.14 to 3.75 mg/100g fresh weight, whereas per cent moisture ranged from 86.50 (NVRS 10:001818) to 97.32 (Sheetal). Positive correlation was observed between total carotenoids and lycopene, chlorophyll b with chlorophyll a, total chlorophyll with both chlorophyll a and b, FRAP with CUPRAC and phenols with total chlorophyll, chlorophyll a and b. Maximum phenotypic and genotypic coefficients of variance were calculated for FRAP (165.98, 165.98) followed by CUPRAC (122.10,122.10) and lycopene content (83.33, 80.84), respectively. These genotypes can be further utilized for development of multinutrient rich varieties. Regular consumption of lettuce can go a long way in tackling osteoporosis, anemia, and cardiovascular related problems.

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References

Apax R, Guclu K, Ozyurek M and Karademir S E. 2004. A novel total antioxidant capacity index for dietary polyphenols, vitamins C and E, using their cupric ion reducing capability in the presence of neocuproine: CUPRAC method. Journal of Agricultural and Food Chemistry 52: 970–81. DOI: https://doi.org/10.1021/jf048741x

Barnes J D, Balaguer L, Maurigue E, Elvira S and Davidson A W. 1992. A reappraisal of the use of DMSO for the extraction and determination of chlorophyll a and b in lichens and higher plants. Environment Experimental Botany 32: 87–99. DOI: https://doi.org/10.1016/0098-8472(92)90034-Y

Benzie F F and Strain J J. 1999. Ferric reducing antioxidant power assay: direct measure of total antioxidant activity of biological fluids and modified version for simultaneous measurement of total antioxidant power and ascorbic acid concentration. Methods in Enzymology 299: 15–23. DOI: https://doi.org/10.1016/S0076-6879(99)99005-5

Bunning M and Kendall P. 2012. Salad greens: Health benefits and safe handling. Food and Nutrition Series Fact sheet No 9: 373.

Burton G W. 1952. Quantitative inheritance in grasses. Proceedings of Sixth International Grassland Congress 7: 277–83.

Deighton N, Brennan R, Finn C and Davies H V. 2000. Antioxidant properties of domesticated and wild Rubus species. Journal of the Science of Food and Agriculture 80: 1 307–13. DOI: https://doi.org/10.1002/1097-0010(200007)80:9<1307::AID-JSFA638>3.0.CO;2-P

Dolma T and Gupta A. 2008. Character association and path coefficient analysis in lettuce (Lactuca sativa L.) genotypes under Kashmir condition. Vegetable Science 35: 185–9.

Dupont S, Mondi Z, Willamson G and Price K. 2000. Effect of variety, processing, and storage on the flavonoid glycoside and composition of lettuce and chicory. Journal of Agricultural and Food Chemistry 48: 3 957–64. DOI: https://doi.org/10.1021/jf0002387

Gravios K A and Helms R S. 1992. Path analysis of rice yield and yield components as affected by seedling rate. Agronomy Journal 84: 1–4. DOI: https://doi.org/10.2134/agronj1992.00021962008400010001x

Hanson C H, Robinson H F and Comstock R E. 1956. Biometric studies of yield in segregating population of Korean Lespedezer. Agronomy Journal 48: 268–72. DOI: https://doi.org/10.2134/agronj1956.00021962004800060008x

Heimler D, Isolani L, Vignolini P, Tombelli S and Romani A. 2007. Polyphenol content and antioxidative activity in some species of freshly consumed salads. Journal of Agricultural and Food Chemistry 55: 1 724–9. DOI: https://doi.org/10.1021/jf0628983

Johnson H W, Robinson H F and Comstock R E. 1955. Estimation of genetic and environmental variability in soybean. Agronomy Journal 47: 314–8. DOI: https://doi.org/10.2134/agronj1955.00021962004700070009x

Kang H M and Saltveit M E. 2002. Antioxidant capacity of lettuce leaf tissue increases after wounding. Journal of Agriculture and Food Chemistry 50: 7 536–41. DOI: https://doi.org/10.1021/jf020721c

Lebeda A, Ryder E J, Grube R, Dolezalova I and Kristkova E. 2007. Lettuce. In: Genetic Resources, Chromosome Engineering and Crop Improvement, Vegetable Crops, pp 377–82. CRC Press, Taylor and Francis, Boca Raton. DOI: https://doi.org/10.1201/9781420009569.ch9

Lee K and Kader A. 2000. Preharvest and postharvest factors influencing vitamin C content of horticultural crops. Postharvest Biology and Technology 20: 207–20. DOI: https://doi.org/10.1016/S0925-5214(00)00133-2

Liu S, Ardo M, Bunning J, Parry K and Zhou C. 2007. Total phenolic content and DPPH radical scavenging activity of lettuce (Lactuca sativa L.) grown in Colorado. Food Science and Technology 40: 552–7. DOI: https://doi.org/10.1016/j.lwt.2005.09.007

Liu W X and Coa S C. 1993. A study on evaluation and related characteristic of non-heading Chinese cabbage and under summer high temperature. Journal of Shandong Agricultural University 24: 176–82.

Lush J L. 1949. Heritability of quantitative characters in farm animals. Heriditas 35: 256–61.

Manach C, Scalbert A, Morand C, Remesy C and Jimenez L. 2004. Polyphenols: Food sources and bioavailability. American Journal of Clinical Nutrition 79: 727–47. DOI: https://doi.org/10.1093/ajcn/79.5.727

Mou B and Ryder E J. 2004. Relationship between the nutritional value and the head structure of lettuce. Acta Horticulturae 637: 361–7. DOI: https://doi.org/10.17660/ActaHortic.2004.637.45

Nicolle C, Carnat A, Fraisse D, Lamaison J L, Rock E and Michel H. 2004. Characterization and variation of antioxidant micronutrients in lettuce (Lactuca sativa). Journal of the Science of Food and Agriculture 84: 2 061–9. DOI: https://doi.org/10.1002/jsfa.1916

Panse V G and Sukhatme P V. 1967. Statistical Methods for Agriculture Workers, p 381. ICAR Publication, New Delhi.

Ranganna S. 1986. Handbook of Analysis and Quality Control for Fruit and Vegetable Products, pp 84–99.Tata Mc Graw Hill Publishing company Ltd, New Delhi.

SAS. 2010. Statistical Analysis Software System, Version 9.3. SAS Institute, Cary, NC, USA.

Sharma J P. 2002. Crop Production Technology for Cold Arid Region, pp 220–6. Kalyani Publisher, Ludhiana, New Delhi.

Shyamala B N, Gupta S, Lakshmi J A and Prakash J. 2005. Leafy vegetable extracts-antioxidant activity and effect on storage stability of heated oils. Innovative Food Science and Emerging Technologies 6: 239–45. DOI: https://doi.org/10.1016/j.ifset.2004.12.002

Singleton V L and Rossi J A. 1965. Colorimetry of total phenolics with phosphomolybdic-phosphotungstic acid reagent. American Journal of Enology Viticulture 16: 144–58. DOI: https://doi.org/10.5344/ajev.1965.16.3.144

Szeto Y T, Kwok T C and Benzie I F. 2004. Effects of a long term vegetarian diet on biomarkers of antioxidant status and cardiovascular disease risk. Nutrition 20: 863-66. DOI: https://doi.org/10.1016/j.nut.2004.06.006

Tomas-Barberan F A, Ferreres F and Gil M I. 2000. Antioxidant phenolic metabolites from fruit and vegetables and changes during postharvest storage and processing. (In) Bioactive and Natural Products, Part D, pp 739–95. Rahman A (Ed.). Elsevier Science, Amsterdam, The Netherlands. DOI: https://doi.org/10.1016/S1572-5995(00)80141-6

Usmani G, Pravin H, Mishra Y and Mandal A K. 2014. Variability, heritability and character association analysis of chemoagronomic traits in Rauwolfia serpentina. Euphytica 200: 259–68. DOI: https://doi.org/10.1007/s10681-014-1153-x

Varalakshmi B and Reddy V V P. 1997. Variability, heritability and correlation studies in vegetable amaranth. Indian Journal of Horticulture 54: 167–70.

Yuan H, Sun Y, Yuan H L and Sun Y W. 2001. Correlation and path and path analysis of some agronomic characters of savoy Chinese cabbage (Brassica chinensis var. rosularis). China Vegetables 5: 17–8.