Gas chromatography-Mass spectrometry analysis for profiling of essential oil constituents derived from leaves of French marigold (Tagete patula)
298 / 143
Keywords:
Essential oil, GC-MS, Hydrodistillation, Leaves, Tagetes patulaAbstract
Different genotypes of marigold (Tagetes sp) belonging to T. patula were investigated for essential oil content in leaves by hydro distillation and examined by gas chromatography (GC) and GC-mass spectrometry (GC-MS). Results indicated that there were wide variations in genotypes for essential oil yield and chemical composition. Essential oil content in leaves of T. patula ranged from 0.08 to 0.15% on fresh weight basis. The maximum essential oil content in leaves was found in cultivar Boy-o-Boy followed by Dainty Marietta , Gulzafri Orange, Bolero Red, Gulzafri Yellow. The essential oil of leaves contained higher amounts of terpinolene, (Z)-β-ocimene, piperitone, piperitone, limonene, (E)-ocimenoxide, β-caryophyllene, (E)-β-ocimene,(Z)-ocimenone and (E)-tagetone. The results of our study clearlyshowed the presence of wide variations in essential oil yieldcontent in the leaves. The genotypes showed very similar qualitative oil composition and differing only in their relative essential oil percentages. Therefore, thorough screening of genotypes for its phytoconstituents will be beneficial for further crop improvement programme.
Downloads
References
Adams R P. 2001. Identification of essential oil components by gas chromatography/mass spectrometry, carolnstream, 1L.Allured Publishing Corporation, USA.
Annoymus. 1976. Raw Materials: Wealth of India. NISCOM, Council of Scientific and Industrial Research, New Delhi.
Anonymous. 2014. Indian Horticulture Database (NHB). 2015.
Baslas R K and Singh A K. 1980.Chromatographic separation of essential oil of Tagete serecta. Indian Perfumer 24: 163-165.
Davies N W. 1990. Gas chromatography retention indices of monoterpenes and sesquiterpenes on methyl silicone and Carbowax 20 m phases. Journal of Chromatography. 503: 1–24. DOI: https://doi.org/10.1016/S0021-9673(01)81487-4
Dhingra S N and Dhingra D R. 1956.Essential oil of Tagetes patula. Perfumer, Journal of Essential Oil Research 47: 391–4.
Hethelyi E, Tetenyi P, Kaposi P, Danos B, Kernoczi Z S, Buki G Y and Koczka I. 1988. GC/MS investigation of antimicrobial and repellent compounds, Herba Hung. 27: 89–105.
Igolen G. 1936. Tagetes. perfumer14: 6–11.
Isiaka A, Ogunwande and Nureni O. 2006. The essential oil from the leaves and flowers of African marigold, Tagetes erecta L. Journal of Essential Oil Research 18(4): 366–8. DOI: https://doi.org/10.1080/10412905.2006.9699115
Krishna A, Kumar S, Mallavarapu G R and Ramesh S. 2004. Composition of the essential oils of the leaves and flowers of Tagetes erecta L. Journal of Essential Oil Research 16: 520–2. DOI: https://doi.org/10.1080/10412905.2004.9698786
Machado M I L, Silva M G V, Matos F J A, Craveiro A A and Alencar J W. 1994. The presence of Indole as minor constituents of Tageteserecta leaf oil.Journal of Essential Oil Research 6: 203–5. DOI: https://doi.org/10.1080/10412905.1994.9698358
Marotti M, Piccaglia R, Biavati B and Marotti I. 2004. Chracterization and yield evaluation of essential oils from different Tagetes species.Journal of Essential Oil Research 6: 440–4. DOI: https://doi.org/10.1080/10412905.2004.9698767
Rondon M, Velasco J, Morales A, Rojas J, Carmona J, Gualtieri M and Hernández V. 2005. Composition and antibacterial activity of the essential oil of Salvia leucantha Cav. cultivated in Venezuela Andes. RevistaLatinoamericana de Química. 33: 40–4.
Rydberg P A. 1915. Tagetes, North Am. Flora 34: 148–59.
Sigh P, Krishna A, Kumar V, Krishna S, Sigh K, Gupta M and Singh S. 2016. Chemistry and biology of industrial crop Tagetes species: A review. Journal of Essential Oil Research 28(1): 1–14. DOI: https://doi.org/10.1080/10412905.2015.1076740
SzarkaSz, He E , Thelyi E, Lemberkovics I N, Kuzovkina P Ba and Nyai E. 2006. GC and GC-MS studies on the essential oil and thiophenes from Tagetes patula L. Chromatographia Supplement 63. DOI: https://doi.org/10.1365/s10337-006-0729-6
Downloads
Submitted
Published
Issue
Section
License
Copyright (c) 2017 The Indian Journal of Agricultural Sciences

This work is licensed under a Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International License.
The copyright of the articles published in The Indian Journal of Agricultural Sciences is vested with the Indian Council of Agricultural Research, which reserves the right to enter into any agreement with any organization in India or abroad, for reprography, photocopying, storage and dissemination of information. The Council has no objection to using the material, provided the information is not being utilized for commercial purposes and wherever the information is being used, proper credit is given to ICAR.