Microwave assisted alkali pretreatment of fruit peel wastes for enzymatic hydrolysis

GARIMA TIWARI; ABHISHEK SHARMA; MANU DALELA; RISHI GUPTA; SATYAWATI SHARMA; RAMESH CHANDER KUHAD

doi:10.56093/ijas.v87i4.69413

Authors

GARIMA TIWARI Indian Institute of Technology Delhi, Hauz Khas, New Delhi 110 016
ABHISHEK SHARMA Indian Institute of Technology Delhi, Hauz Khas, New Delhi 110 016
MANU DALELA Indian Institute of Technology Delhi, Hauz Khas, New Delhi 110 016
RISHI GUPTA Indian Institute of Technology Delhi, Hauz Khas, New Delhi 110 016
SATYAWATI SHARMA Indian Institute of Technology Delhi, Hauz Khas, New Delhi 110 016
RAMESH CHANDER KUHAD Indian Institute of Technology Delhi, Hauz Khas, New Delhi 110 016

https://doi.org/10.56093/ijas.v87i4.69413

Keywords:

Enzymatic hydrolysis, Fruit peel wastes, Microwave assisted alkali pretreatment, Reducing sugars

Abstract

The present study proved the potential of generating reducing sugars from various fruit peel wastes (pineapple, mango, orange) using microwave assisted alkali pretreatment. The results showed that enzymatic hydrolysis of pretreated pineapple peel waste produced maximum reducing sugar, i.e. 0.774 g/g dry biomass. X-ray diffraction
study revealed the prominent role of microwave heating in the disruption of recalcitrant lignocellulosic structures and improving the enzymatic digestibility of FPWs. We believe that microwave assisted alkali pretreatment of FPWs could be an energy-saving technology aimed to valorize the agro-industrial wastes in a cost effective way.

Downloads

Download data is not yet available.

References

Binod P, Satyanagalakshmi K, Sindhu R, Janu K U, Sukumaran R K and Pandey A.2012. Short duration microwave assisted pretreatment enhances the enzymatic saccharification and fermentable sugar yield from sugarcane bagasse. Renewable Energy 37: 109–16. DOI: https://doi.org/10.1016/j.renene.2011.06.007

Choi I S, Lee Y G, Khanal S K, Park B J and Bae H J.2015. A low-energy, cost-effective approach to fruit and citrus peel waste processing for bioethanol production. Applied Energy 140: 65–74. DOI: https://doi.org/10.1016/j.apenergy.2014.11.070

Goering H K and Van Soest P J.1975. Forage fiber analysis. Agriculture Research Series Handbook, p 379.

Gong G, Liu D and Huang Y.2010. Microwave-assisted organic acid pretreatment for enzymatic hydrolysis of rice straw. Biosystem Engineering 107: 67–73. DOI: https://doi.org/10.1016/j.biosystemseng.2010.05.012

Gupta R, Sharma K K and Kuhad R C.2010.Separate hydrolysis and fermentation (SHF) of Prosopis juliflora, a woody substrate, for the production of cellulosic ethanol by Saccharomyces cerevisiae and Pichia stipitis-NCIM 3498. Bioresource Technology 100: 1 214–20. DOI: https://doi.org/10.1016/j.biortech.2008.08.033

Gothwal R, Gupta A, Kumar A, Sharma S and Alappat B J.2012. Feasibility of dairy waste water (DWW) and distillery spent wash (DSW) effluents in increasing the yield potential of Pleurotus flabellatus (PF 1832) and Pleurotus sajor-caju (PS 1610) on bagasse. 3 Biotech 2: 249–57. DOI: https://doi.org/10.1007/s13205-012-0053-9

Miller G M.1959.Use of dinitrosalicylic acid reagent for determination of reducing sugar. Analytical Chemistry 31: 426–8. DOI: https://doi.org/10.1021/ac60147a030

Nomanbhay S M, Hussain R and Palanisamy K.2013.Microwave-assisted alkaline pretreatment and microwave assisted enzymatic saccharification of oil palm empty fruit bunch fiber for enhanced fermentable sugar yield. Journal of Seismology and Earthquake Engineering 3: 7–17. DOI: https://doi.org/10.4236/jsbs.2013.31002

Park S, Baker J O, Himmel M E, Parilla P A and Johnson D K.2010. Cellulose crystallinity index: measurement techniques and their impact on interpreting cellulase performance. Biotechnology for Biofuels 3: 1-10. DOI: https://doi.org/10.1186/1754-6834-3-10

Reddy L V, Reddy O V S and Wee Y J.2011. Production of ethanol from mango (Mangifera indica L.) peel by Saccharomyces cerevisiae CFTRI101. African Journal of Biotechnology 10: 4 183–9.

Segal L, Greely J J, Martin A E J and Conrad L M.1959.An empirical method for estimating the degree of crystallinity of native cellulose using X-ray diffractometer. Textile Research Journal 29: 780–94. DOI: https://doi.org/10.1177/004051755902901003

Updegraff D M.1969. Semi micro determination of cellulose in biological materials. Analytical Biochemistry 32: 420–4. DOI: https://doi.org/10.1016/S0003-2697(69)80009-6

Xuebin L, Bo X, Yimin Z and Irini A.2011. Microwave pretreatment of rape straw for bioethanol production: Focus on energy efficiency. Bioresource Technology 102: 7 937–40. DOI: https://doi.org/10.1016/j.biortech.2011.06.065

Zentek J, Knorr F and Mader A.2014.Reducing waste in fresh produce processing and households through use of waste as animal feed. (In) Global Safety of Fresh Produce: A Handbook of Best Practice, pp 140–52 Innovative Commercial Solutions and Case Studies. DOI: https://doi.org/10.1533/9781782420279.2.140