Molecular characterization of African marigold (Tagetes erecta) genotypes using RAPD markers

SAPNA PANWAR; KANWAR PAL SINGH; T JANAKIRAM; C BHARADWAJ; NAMITA NAMITA; HUMA SONAH; R K DESHMUKH; T R SHARMA

doi:10.56093/ijas.v87i5.70190

Authors

SAPNA PANWAR ICAR- Indian Agricultural Research Institute, New Delhi 110 012
KANWAR PAL SINGH ICAR- Indian Agricultural Research Institute, New Delhi 110 012
T JANAKIRAM ICAR- Indian Agricultural Research Institute, New Delhi 110 012
C BHARADWAJ ICAR- Indian Agricultural Research Institute, New Delhi 110 012
NAMITA NAMITA ICAR- Indian Agricultural Research Institute, New Delhi 110 012
HUMA SONAH ICAR- Indian Agricultural Research Institute, New Delhi 110 012
R K DESHMUKH ICAR- Indian Agricultural Research Institute, New Delhi 110 012
T R SHARMA ICAR- Indian Agricultural Research Institute, New Delhi 110 012

https://doi.org/10.56093/ijas.v87i5.70190

Keywords:

Genetic diversity, RAPD, Tagetes erecta, UPGMA

Abstract

The present investigation was carried out to characterize 22 genotypes of marigold (Tagetes erecta L.) with RAPD (randomly amplified polymorphic DNA) markers. A total of 26 RAPD markers were utilized for assessment of genetic divergence in marigold which amplified a total of 241 amplicons, out of which 238 (98.80%) were found polymorphic. The average number of amplicon per primer was 9.27. The Jaccard's similarity coefficient ranged from
0.18 to 0.80. The mean values of polymorphic information content (PIC), resolving power (RP) and marker index (MI) of RAPD primers were 0.38, 8.36 and 3.44 respectively. Cluster analysis was done by unweighted pair group method for arithmetic average (UPGMA) and Principal Component analysis (PCA) was also done for the grouping of genotypes. The broad range of Jaccard's similarity coefficient observed for the genotypic data generated suggest the wide genetic base for marigold genotypes which will be effectively explored for future breeding programmes.

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