Soybean (Glycine max) genotype–mediated variation in the symbiotic performance of Rhizobium


Abstract views: 185 / PDF downloads: 84

Authors

  • SATISH NAIK S K ICAR-Indian Agricultural Research Institute, New Delhi 110 012
  • K ANNAPURNA ICAR-Indian Agricultural Research Institute, New Delhi 110 012
  • ANNU KUMARI ICAR-Indian Agricultural Research Institute, New Delhi 110 012
  • L VITHAL ICAR-Indian Agricultural Research Institute, New Delhi 110 012
  • K K REDDY ICAR-Indian Agricultural Research Institute, New Delhi 110 012
  • K SWARNALAKSHMI ICAR-Indian Agricultural Research Institute, New Delhi 110 012

https://doi.org/10.56093/ijas.v87i8.73238

Keywords:

Acetylene reducing activity, Bradyrhizobium, Genotype, Sinorhizobium, Soybean

Abstract

Legume–rhizobium interaction is the result of molecular dialogue involving a succession of events on the perception of signal molecules produced and secreted by both partners. Soybean (Glycine max L.) is known to be nodulated by two different genera, Bradyrhizobium (slow growing rhizobia) and Sinorhizobium (fast growing) species. The present investigation is an attempt to understand the host genotypic effect on the fast and slow growing root nodulating bacteria of soybean which impacts the biological nitrogen fixation and would lead to the selection of best cultivar-strain compatible interaction. Five soybean genotypes of North Plain Zone, viz. DS 12-13, DS 9712, DS 2705, SL 979, SL 982 were evaluated for their symbiotic potential with two slow (KAS-1, MTCC10753) and two fast growing root nodulating bacteria (DS-1, LSR-8). Genotype DS12-13 formed significant number of nodules with KAS-1 with LS mean of 17.3. Genotype DS 2705 was poorly nodulated by the four strains. Among the strains, slow growing KAS-1 and fast growing DS-1 strains were effective across the genotypes. There was significant increase in the specific acetylene reducing activity of these strains 71.1 and 72.6 nmoles of C2H4 produced/mg/ndw respectively. These strains conformed to the biochemical identification by failing to grow on citrate, glucose peptone agar and Hoffer's alkaline media.

Downloads

Download data is not yet available.

References

Annapurna K, Balakrishnan N and Vital L. 2007.Verification and rapid identification of soybean rhizobia in Indian soils. Current Microbiology 54: 287–91. DOI: https://doi.org/10.1007/s00284-006-0423-9

Appunu C, Sen D, Singh M K and Dhar B. 2008.Variation in symbiotic performance of Bradyrhizobium japonicum strains and soybean cultivars under field conditions. Journal of Central European Agriculture 9: 185–90.

Benjamin G, Fathi B, Pascal R and Gary S. 2015. Rhizobium– legume symbioses: the crucial role of plant immunity. Trends in Plant Science 2: 186–94. DOI: https://doi.org/10.1016/j.tplants.2014.11.008

Biate D L, Kumari A, Annapurna K, Vithalkumar L, Ramadoss D, Reddy K K and Naik S. 2014a. Legume root exudates: Their role in symbiotic interactions. (In) Plant Microbes Symbiosis: Applied Facets, pp 259–71. (Ed.) Naveen Arora. Springer Verlag Publications. DOI: https://doi.org/10.1007/978-81-322-2068-8_13

Biate D L, Vithalkumar L, Ramadoss D, Kumari A, Naik S, Reddy K K and Annapurna K. 2014b. Genetic diversity of soybean root nodulating bacteria. (In) Bacterial Diversity in Sustainable Agriculture Sustainable Development and Biodiversity, Vol 1, pp 131-45. Maheshwari D K. Springer Verlag Publications. DOI: https://doi.org/10.1007/978-3-319-05936-5_5

Chen W X, Yan G H and Li J L 1988. Numerical taxonomic study of fast-growing soybean rhizobia and a proposal that Rhizobium fredii be assigned to Sinorhizobium gen. Nov. International Journal of Systematic Bacteriology 38: 392–7. DOI: https://doi.org/10.1099/00207713-38-4-392

Hardy RW F, Burns R C and Holstein R D. 1968. Applications of the acetylene-ethylene assay for measurement of nitrogen fixation. Plant Physiology 43: 1 185–207.

Jordan D C. 1982. Transfer of Rhizobium japonicum Buchana 1980 to Bradyrhizobium gen. Nov., genus of slow growing and root nodules bacteria from leguminous plants. International Journal of Systematic Bacteriology 32: 136–9. DOI: https://doi.org/10.1099/00207713-32-1-136

Maseko S T, Mathews and, Dakora. 2015. Differences in plant growth, nodulation and intrinsic water use efficiency of Rhizobium-inoculated and un-inoculated promiscuous and commercial soybean genotypes grown at Mpumalanga, South Africa. South African Journal of Botany 98: 189–90. DOI: https://doi.org/10.1016/j.sajb.2015.03.082

Patten C L and Glick B R. 2002. Role of Pseudomonas putida indole acetic acid in development of the host root system. Applied and Environmental Microbiology 66: 3795–801. DOI: https://doi.org/10.1128/AEM.68.8.3795-3801.2002

Pueppke S G and Broughton W J. 1999. Rhizobium sp NGR234 and R. fredii USDA257 share exceptionally broad, nested host ranges. Molecular Plant-Microbe Interactions 12: 293–18. DOI: https://doi.org/10.1094/MPMI.1999.12.4.293

Ronner E , Franke A C, Vanlauwe B, Dianda M, Edeh E, Ukem B, Bala A, van Heerwaarden J and Giller K E. 2016. Understanding variability in soybean yield and response to P-fertilizer and rhizobium inoculants on farmers’ fields in northern Nigeria. Field Crops Research 186: 133–45. DOI: https://doi.org/10.1016/j.fcr.2015.10.023

Sangam, L. Dwivedi, Kanwar L. Sahrawat, Hari D and Upadhyaya. 2015. Advances in host plant and Rhizobium genomics to enhance symbiotic nitrogen fixation in grain legumes. Advances in Agronomy 129: 1–116. DOI: https://doi.org/10.1016/bs.agron.2014.09.001

Sameh H. Youseif Fayrouz H, Abd El-Megeed, AmrAgeez, Zeinat K and Mohamed. 2014. Phenotypic characteristics and genetic diversity of rhizobia nodulating soybean in Egyptian soils. European Journal of Soil Biology 60: 34–43. DOI: https://doi.org/10.1016/j.ejsobi.2013.10.008

Satyaprakash Ch and Annapurna K. 2006. Diversity of a soybean Bradyrhizobial population adapted to an Indian soil. Journal of Plant Biochemistry and Biotechnology 15: 27–32. DOI: https://doi.org/10.1007/BF03321897

Simms E L and Taylor D L. 2002.Partner choice in nitrogen-fixation mutualisms of legumes and rhizobia. Integrative and Comparative Biology 42: 369–80. DOI: https://doi.org/10.1093/icb/42.2.369

Vincent J M. 1970. A manual for the practical study of root nodule bacteria. IBP Handbook No. 15, Blackwell Scientific Publications, Oxford, p 164.

Downloads

Submitted

2017-08-16

Published

2017-08-17

Issue

Section

Articles

How to Cite

K, S. N. S., ANNAPURNA, K., KUMARI, A., VITHAL, L., REDDY, K. K., & SWARNALAKSHMI, K. (2017). Soybean (Glycine max) genotype–mediated variation in the symbiotic performance of Rhizobium. The Indian Journal of Agricultural Sciences, 87(8), 1051–1054. https://doi.org/10.56093/ijas.v87i8.73238
Citation