Effect of extraction method and thermal processing on retention of bioacvtive compounds of pomegrante (Punica granatum) (cv. Bhagwa) juice


266 / 124

Authors

  • NILESH N GAIKWAD
  • R K PAL
  • SWATI SURYAWANSHI
  • K D BABU
  • ASHIS MAITY
  • SUSHEEL SARKAR

https://doi.org/10.56093/ijas.v87i11.75679

Keywords:

Bioactive compounds, Juice, Pomegranate, Shelf life, Thermal treatment

Abstract

The effect of juice extraction methods (from arils and halved fruits), temperature (70, 80, 90 and 100ÌŠC) and thermal processing time (2, 5 and 10 minutes) on quality of the pomegranate (Punica granatum L.) juice has been studied. The quality parameters studied included biochemical parameters such as ascorbic acid, total anthocyanin content, total phenolic compounds, antioxidant capacity, TSS, and acidity. The color, microbial, and sensory quality
was also evaluated. The results revealed that thermal treatment reduces important bioactive compound with increase in temperature and time of processing. The thermal processing at 80 ÌŠC for 5 min. found to be suitable for reducing microbial load to below detectable limits and maintaining sensory quality up to 7.91 on ten point hedonic scale. The juice extracted from halved fruits was found to be nutritionally rich than juice extracted from arils in terms of antioxidant capacity, ascorbic acid, total phenolic compounds, total anthocyanin and color value a*. The retention
of antioxidant capacity, total phenolic compounds,total anthocyanin content, ascorbic acid and color for thermal processing at 80 ÌŠC for 5 minutes for juice extracted from halved fruits was 22.68 mg/100ml AAE, 2189 mg/l GAE, 3.18 mg/100 ml, 18.33 mg/100 ml and 37.05 respectively.

Downloads

Download data is not yet available.

Author Biographies

  • NILESH N GAIKWAD
    Scientist, ICAR-National Research Centre on Pomegranate, Solapur, Maharashtra 413 255
  • R K PAL
    Director, ICAR-National Research Centre on Pomegranate, Solapur, Maharashtra 413 255
  • SWATI SURYAWANSHI
    Research Associate, ICAR-National Research Centre on Pomegranate, Solapur, Maharashtra 413 255
  • K D BABU
    Principal Scientist, ICAR-National Research Centre on Pomegranate, Solapur, Maharashtra 413 255

  • ASHIS MAITY
    Scientist, ICAR-National Research Centre on Pomegranate,
    Solapur 413 255.
  • SUSHEEL SARKAR
    Scientist, ICAR-Indian Agricultural Statistical Research Institute, New Delhi 110 012.

References

Ainsworth E A and Gillespie K M. 2007. Estimation of total phenolic content and other oxidation substrates in plant tissues using folin-ciocalteu reagent. Nature Protocols 2: 875–7. DOI: https://doi.org/10.1038/nprot.2007.102

Alighourchi H R, Barzegar M, Sahari M A and Abbasi S. 2013. Effect of sonication on anthocyanins, total phenolic content, and antioxidant capacity of pomegranate juices. International Food Research Journal 20(4): 1703–9.

Al-Maiman S A and Ahmad D. 2002.Changes in physical and chemical properties during pomegranate (Punica granatum L.) fruit maturation. Food Chemistry 67: 437–141. DOI: https://doi.org/10.1016/S0308-8146(01)00301-6

Alper N, Bahçeci K S¸ and Acar J. 2005. Influence of processing and pasteurization on color values and total phenolic compounds of pomegranate juice. Journal of Food Processing and Preservation 29: 357–68. DOI: https://doi.org/10.1111/j.1745-4549.2005.00033.x

Al-Said F A, Opara L U and Al-Yahyai R A. 2009.Physico-chemical and textural quality attributes of pomegranate cultivars (Punica granatum L.) grown in the Sultanate of Oman. Journal of Food Engineering 90: 129–34. DOI: https://doi.org/10.1016/j.jfoodeng.2008.06.012

AOAC. 1984.Official Methods of Analysis. Association of Official Analytical Chemists, Washington.

AOAC. 2000. Official Methods of Analysis, 17th Edition. Association of Official Analytical Chemists (AOAC), Washington DC, USA.

Aviram M, Dornfeld L, Rosenblat M, Volkova N, Kalplan M, Coleman R, Hayek T, Presser D and Fuhrman B. 2000. Pomegranate juice consumption reduces oxidative stress, atherogenic modifications to LDL and platelet aggregation, Studies in humans and in atheroscierotic E- deficient mice. American Journal of Clinical Nutrition 71: 1062–76. DOI: https://doi.org/10.1093/ajcn/71.5.1062

Aviram M, Rosenblat M and Gaitini D. 2004. Pomegranate juice consumption for 3 years by patients with carotid artery stenosis reduces common carotid intima-media thickness, blood pressure and LDL oxidation. Clinical Nutrition 23(3): 423–33. DOI: https://doi.org/10.1016/j.clnu.2003.10.002

Barros Z M P, Salgado J M, Melo P S and Biazotto F O. 2014. Enrichment of commercially-prepared juice with pomegranate (Punica granatum L.) peel extract as a source of antioxidants. Journal of Food Research 3: 6179–87. DOI: https://doi.org/10.5539/jfr.v3n6p179

Benzie I F F and Strain J J. 1996. The ferric reducing ability of plasma (FRAP) as a measure of antioxidant power. The FRAP assay. Analytical Chemistry 239: 70–6. DOI: https://doi.org/10.1006/abio.1996.0292

Dar G H, Zargar Y and Shah G H. 1992. Effect of processing operations and heat treatment on physico-chemical characteristics and microbiological load of apple juice concentrate. Indian Food Packer 46: 45–50.

El-Nemr S E, Ismail I A and Ragab M. 1992.The chemical composition of juice and seeds of pomegranate fruits. Fruit Processing 2(11): 162–4.

FDA 1992. Bacteriological analytical manual, 6th Edn.Association of Official Analytical Chemists, Food and Drug Administration. Arlington, VA.

Food safety and standards (Food product standards and food additives) regulation, 2011 (part II), Ministry of Health And Family Welfare (Food Safety and Standards Authority of India) Notification, New Delhi, dated the 1st August, 2011.

Gaikwad N N, Pal R K and Babu K D. 2014.Entrepreneurship development in pomegranate through value addition. Souvenir National Seminar on Pomegranate for Nutrition, Livelihood Security and Entrepreneurship Development, 5-7 December 2014, Solapur, pp 264–71.

Ghorai K and Khurdiya D S. 1998. Storage of heat processed Kinnow mandarin juice. Journal of Food Science Technology 35: 422–4.

Gil M I, Tomas-Barberan F A, Hess-Pierce B, Holcroft D M and Kader A A. 2000. Antioxidant activity of pomegranate juice and its relationship with phenolic composition and processing. Journal of Agriculture and Food Chemistry 48: 4581–9. DOI: https://doi.org/10.1021/jf000404a

Harris L J and S N Ray. 1935. Determination of plasma ascorbic acid by 2, 6-dichlorophenol indophenol titration. Lancet 1: 462.

Heyn B. 1990. Ayurveda: The Indian Art of Natural Medicine and Life Extension, pp 178. Healing Art Press, Rochester, Vermont.

Janbi A H A and Al-Said S A. 2014. The effect of technological treatments of the pasteurized juice processing on the antioxidant compounds content and the antioxidant activity of pomegranate (Punica granatum L.) fruits juice. Middle East Journal of Applied Sciences 4(2): 232–42.

Lansky P E and Newman R A. 2007. Punica granatum (pomegranate) and its potential for prevention and treatment of inflammation and cancer. Journal of Ethnopharmacology 109: 177–206. DOI: https://doi.org/10.1016/j.jep.2006.09.006

Li Y, Guo C, Jijun Y, Wei J, Xu J, and Cheng S. 2006. Evaluation of antioxidant properties of pomegranate peel extract in comparison with pomegranate pulp extract. Food Chemistry 96(2): 254–60. DOI: https://doi.org/10.1016/j.foodchem.2005.02.033

Melgarejo P, Salazar D M and Art´es F. 2000.Organic acids and sugars composition of harvested pomegranate fruits. European Food Research and Technology 211(3): 185–90. DOI: https://doi.org/10.1007/s002170050021

Mohan M, Waghulde H and Kasture S. 2010. Effect of pomegranate juice on Angiotensin II-induced hypertension in diabetic Wistar rats. Phytotherapy Research 24: 196–203. DOI: https://doi.org/10.1002/ptr.3090

Pareek S, Paliwal R and Mukherjee S. 2011.Effect of juice extraction methods and processing temperature-time on juice quality of Nagpur mandarin (Citrus reticulata Blanco) during storage. Journal of Food Science and Technology 48(2): 197–203. DOI: https://doi.org/10.1007/s13197-010-0154-6

Patricia C. 1995. Test 17.2.01, pp 3-4. Official Method of Analysis of AOAC International, 16th Edn. AOAC International, Virginia, USA,

Paul R. and Ghosh U. 2012.Effect of thermal treatment on ascorbic content of pomegranate juice. Indian Journal of Biotechnology 11: 309–13.

Ranganna S. 2000. Handbook of Analysis and Quality Control for Fruits and Vegetable Products, p 1152, 2nd edn. Tata McGraw- Hill Publishing Company Ltd, New Delhi.

Rosenblat M and Aviram M. 2006. Pomegranates: Ancient Roots to Modern Medicine, In Vitro Studies, pp 31–43. Seeram N P and Heber D (Eds.). Taylor and Francis Group, New York.

Sadasivam and Balasubramanian T. 1987. Practical Manual in Biochemistry, pp 14. Tamil Nadu Agricultural University, Coimbatore.

Shiban M S, Al-Otaibi M M, and Al-Zoreky N S. 2012 Antioxidant activity of pomegranate (Punica granatum L.) fruit peels. Food and Nutrition Sciences 3: 991–6. DOI: https://doi.org/10.4236/fns.2012.37131

Shwartz E, Glazer I, Bar-Ya'akov I, Matityahu I, Bar-Ilan I, Holland D and Amir R. 2009a.Changes in chemical constituents during the maturation and ripening of two commercially important pomegranate accessions. Food Chemistry 115: 965–73. DOI: https://doi.org/10.1016/j.foodchem.2009.01.036

Shwartz E, Tzulker R, Glazer I, Bar-Ya'akov I, Wiesman Z, Tripler E, Bar-Ilan I, Fromm H, Neori H B, Holland D and Amir R. 2009b. Environmental conditions affect the color, taste, and antioxidant capacity of 11 pomegranate accessions’ fruits. Journal of Agricultural and Food Chemistry 57: 9197–209. DOI: https://doi.org/10.1021/jf901466c

Singleton V L and Rossi J A J. 1965.Colorimetry of total phenolics with phospomolybdic – phospotungstic acid reagents. American Journal of Enology and viticulture, 16: 144–58.

Stowe C B. 2011. The effects of pomegranate juice consumption on blood pressure and cardiovascular health. Complementary Therapies in Clinical Practice 17: 113–5. DOI: https://doi.org/10.1016/j.ctcp.2010.09.004

Tezcan F, Gültekin-Özgüven M, Diken T, Ozçelik B and Erim F B. 2009. Antioxidant activity and total phenolic, organic acid and sugar content in commercial pomegranate juices. Food Chemistry 115(3): 873–7. DOI: https://doi.org/10.1016/j.foodchem.2008.12.103

Vanderzant C and Splittstroesser D F. 1992. Compendium of Methods for the Microbiological Examination of Foods, pp 75–87. Washington DC.

Wrolstad R E, Durst R W and Lee J. 2005.Tracking color and pigment changes in anthocyanin products. Trends in Food Science and Technology 16(9): 423–8. DOI: https://doi.org/10.1016/j.tifs.2005.03.019

Downloads

Submitted

2017-11-08

Published

2017-11-13

Issue

Section

Articles

How to Cite

GAIKWAD, N. N., PAL, R. K., SURYAWANSHI, S., BABU, K. D., MAITY, A., & SARKAR, S. (2017). Effect of extraction method and thermal processing on retention of bioacvtive compounds of pomegrante (Punica granatum) (cv. Bhagwa) juice. The Indian Journal of Agricultural Sciences, 87(11), 1445–1152. https://doi.org/10.56093/ijas.v87i11.75679
Citation