Pigmented radish (Raphanus sativus): Genetic variability, heritability and interrelationships of total phenolics, anthocyanins and antioxidant activity
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Authors
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B K SINGH
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T K KOLEY
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PRADIP KARMAKAR
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AJAY TRIPATHI
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BIJENDRA SINGH
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MAJOR SINGH
Keywords:
Anthocyanins, Antioxidants, Correlation, Inheritance, Radish, Raphanus sativus, VariabilityAbstract
Radish (Raphanus sativus L.) is an important salad vegetable grown and consumed throughout the world for fleshy roots which has numerous categories' varying in root colour, size, shape and flavour. The uses of coloured radishes in the salads and their anthocyanins as colourants are gaining popularity because of the colour characteristics, health benefits as well as antioxidant activities. However, information on the genetic variability, heritability and inter-relationship of total phenolics, anthocyanins and antioxidant activities in pigmented radish is very limited, but pre-requisite to initiate breeding programme; and therefore investigated in the present study. Radish genotypes were significantly diverse for all the antioxidants; differed by 4.98-fold for total phenolics, 36.16-fold for anthocyanins content, 4.96-fold for FRAP activity and 4.03-fold for CUPRAC activity; and the genotypes accounted for >97% of total variations. The meager differences between phenotypic and genotypic coefficient of variation reveals the greater role of genotypes and lesser influence of the environment on the biosynthesis and accumulation of antioxidants. Significantly positive correlations along with higher magnitude for anthocyanins content, total phenolics, FRAP activity and CUPRAC activity (r= 0.823 to 0.964) could be used as indirect selection criteria for improving levels of antioxidant compounds. The estimates of heritability and genetic advance indicate the role of additive and non-additive genes for biosynthesis of antioxidants and root development, respectively; therefore, recurrent selection would be the best breeding approach to improve both the traits simultaneously in coloured radish.
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References
Apak R, Guclu K, Ozyurek M and Celik S E. 2008. Mechanism of antioxidant capacity assays and the CUPRAC (cupric ion reducing antioxidant capacity) assay. Microchimica Acta 160: 413–9. DOI: https://doi.org/10.1007/s00604-007-0777-0
Benzie I F F and Strain J J. 1996. The ferric reducing ability of plasma (FRAP) as a measure of “antioxidant power”: The FRAP assay. Analytical Biochemistry 239: 70–6. DOI: https://doi.org/10.1006/abio.1996.0292
Burton G W and DeVane D H. 1953. Estimating heritability in fall fescue from replicated clonal material. Agronomy Journal 4: 78–81.
Cartea M E, Francisco M, Soengas P and Velasco P. 2011. Phenolic compounds in Brassica vegetables. Molecules 16: 251–80. DOI: https://doi.org/10.3390/molecules16010251
Castilla P, Davalos A, Teruel J L, Cerrato F, Fernandez-Lucas M, Merino J L, Sanchez-Martin C C, Ortuno J and Lasuncion M A. 2008. Comparative effects of dietary supplementation with red grape juice and vitamin E on production of superoxide by circulating neutrophil NADPH oxidase in hemodialysis patients. American Journal of Clinical Nutrition 87: 1053–61. DOI: https://doi.org/10.1093/ajcn/87.4.1053
Chalker-Scott L. 1999. Environmental significance of anthocyanins in plant stress responses. Photochemistry and Photobiology 70: 1–9. DOI: https://doi.org/10.1111/j.1751-1097.1999.tb01944.x
Chander S, Meng Y, Zhang Y, Yan J and Li J. 2008. Comparison of nutritional traits variability in selected eighty-seven inbreds from Chinese maize (Zea mays L.) germplasm. Journal of Agricultural and Food Chemistry 56: 6506–11. DOI: https://doi.org/10.1021/jf7037967
Chu Y F, Sun J, Wu X and Liu R H. 2002. Antioxidant and anti-proliferative activities of common vegetables. Journal of Agricultural and Food Chemistry 50: 6910–6. DOI: https://doi.org/10.1021/jf020665f
Connor A M, Stephens M J, Hall H K and Alspach P A. 2005. Variation and heritabilities of antioxidant activity and total phenolic content estimated from a red raspberry factorial experiment. Journal of the American Society for Horticultural Science 130: 403–11. DOI: https://doi.org/10.21273/JASHS.130.3.403
Delgado-Vargas F and Paredes-Lopez O. 2003. Natural Colorants for Food and Nutraceutical Uses. CRC Press, Boca Raton, Florida, USA. DOI: https://doi.org/10.1201/9781420031713
Dixon R A and Palva N L. 1995. Stress-induced phenylpropanoid metabolism. Plant Cell 7: 1085–97. DOI: https://doi.org/10.1105/tpc.7.7.1085
Estruch R, Sacanella E, Badia E, Antunez E, Nicolas J M, Fernandez-Sola J, Rotiliob D, Gaetanoc G D, Rubind E and Urbano-Márqueza A. 2004. Different effects of red wine and gin consumption on inflammatory biomarkers of atherosclerosis: a prospective randomized crossover trial: effects of wine on inflammatory markers. Atherosclerosis 175: 117–23. DOI: https://doi.org/10.1016/j.atherosclerosis.2004.03.006
George R A T and Evans D R. 1981. A classification of winter radish cultivars. Euphytica 30: 483–92. DOI: https://doi.org/10.1007/BF00034013
Ghosh D and Konishi T. 2007. Anthocyanins and anthocyanin-rich extracts: role in diabetes and eye function. Asia Pacific Journal of Clinical Nutrition 16(2): 200–208.
Giusti M M, Rodriguez-Saona L E, Baggett J R, Reed G L, Durst R W and Wrolstad R E. 1998. Anthocyanin pigment composition of red radish cultivars as potential food colorants. Journal of Food Science 63(2): 219–24. DOI: https://doi.org/10.1111/j.1365-2621.1998.tb15713.x
Giusti M M and Wrolstad R E. 1996. Characterization of radish anthocyanins. Journal of Food Science 61: 322–6. DOI: https://doi.org/10.1111/j.1365-2621.1996.tb14186.x
Giusti M M and Wrolstad R E. 2003. Acylated anthocyanins from edible sources and their applications in food systems. Biochemical Engineering Journal 14: 217–25. DOI: https://doi.org/10.1016/S1369-703X(02)00221-8
Gould K S, McKelvie J and Markham K R. 2002. Do anthocyanins function as antioxidants in leaves? Imaging of H2O2 in red and green leaves after mechanical injury. Plant, Cell and Environment 25: 1261–9. DOI: https://doi.org/10.1046/j.1365-3040.2002.00905.x
Hanson P M, Yang R, Wu J, Chen J, Ledesma D and Tsou S C S. 2004. Variation for antioxidant activity and antioxidants in tomato. Journal of the American Society for Horticultural Science 129(5): 704–11. DOI: https://doi.org/10.21273/JASHS.129.5.0704
Harakotr B, Suriharn B, Scott M P and Lertrat K. 2015. Genotypic variability in anthocyanins, total phenolics, and antioxidant activity among diverse waxy corn germplasm. Euphytica 203: 237–48. DOI: https://doi.org/10.1007/s10681-014-1240-z
Holton T A and Cornish E C. 1995. Genetics and biochemistry of anthocyanin biosynthesis. Plant Cell 7: 1071–83. DOI: https://doi.org/10.1105/tpc.7.7.1071
Horbowicz M, Kosson R, Grzesiuk A and Debski H. 2008. Anthocyanins of fruits and vegetables- their occurrence, analysis and role in human nutrition. Vegetable Crops Research Bulletin 68: 5–22. DOI: https://doi.org/10.2478/v10032-008-0001-8
Hu Q P and Xu J G. 2011. Profiles of carotenoids, anthocyanins, phenolics, and antioxidant activity of selected color waxy corn grains during maturation. Journal of Agricultural and Food Chemistry 59: 2026–33. DOI: https://doi.org/10.1021/jf104149q
Iwasaki-Kurashige K, Loyaga-Rendon Y, Matsumoto H, Tokunaga T and Azuma H. 2006. Possible mediators involved in decreasing peripheral vascular resistance with blackcurrant concentrate (BC) in hind-limb perfusion model of the rat. Vascular Pharmacology 44: 215–23. DOI: https://doi.org/10.1016/j.vph.2005.12.001
Jing P, Zhao S, Ruan S, Sui Z, Chen L, Jiang L and Qian B. 2014. Quantitative studies on structure-ORAC relationships of anthocyanins from eggplant and radish using 3D-QSAR. Food Chemistry 145: 365–71. DOI: https://doi.org/10.1016/j.foodchem.2013.08.082
Kallithraka S, Mohdaly A A, Makris D P and Kefalas P. 2005. Determination of major anthocyanin pigments in Hellenic native grape varieties (Vitis vinifera): association with antiradical activity. Journal of Food Composition and Analysis 18: 375–86. DOI: https://doi.org/10.1016/j.jfca.2004.02.010
Kaneko Y and Matsuzawa Y. 1993. Radish (Raphanus sativus L.). (In): Genetic Improvement of Vegetable Crops, pp 487–505. Kalloo G and Bergh B O (Eds). Pergamon Press Ltd., Oxford, England. DOI: https://doi.org/10.1016/B978-0-08-040826-2.50039-4
Kapusta-Duch J, Kopec A, Piatkowska E, Borczak B and Leszczynska T. 2012. The beneficial effects of Brassica vegetables on human health. Roczniki Państwowego Zakładu Higieny 63(4): 389–95.
Karmakar P, Munshi A D, Behera T K, Kumar R, Kaur C and Singh B K. 2013. Hermaphrodite inbreds with better combining ability to improve antioxidant properties in ridge gourd [Luffa acutangula (Roxb.) L.]. Euphytica 191(1): 75–84. DOI: https://doi.org/10.1007/s10681-013-0862-x
Koes R E, Quattrocchio F and Mol J N M. 1993. The flavonoid biosynthetic pathway in plants: function and evolution. Bioessays 16: 123–32. DOI: https://doi.org/10.1002/bies.950160209
Koley T K, Singh S, Khemariya P, Sarkar A, Kaur C, Chaurasia S N S and Naik P S. 2014. Evaluation of bioactive properties of Indian carrot (Daucus carota L.): A chemometric approach. Food Research International 60: 76–85. DOI: https://doi.org/10.1016/j.foodres.2013.12.006
Kramer J H. 2004. Anthocyanosides of Vaccinium myrtillus (bilberry) for night vision—a systematic review of placebo-controlled trials. Survey of Ophthalmology 49: 618. DOI: https://doi.org/10.1016/j.survophthal.2004.08.009
Kumar R, Sharma R, Gupta R K and Singh M. 2014. Determination of genetic variability and divergence for root yield and quality characters in temperate radishes. International Journal of Vegetable Science 18(4): 307–18. DOI: https://doi.org/10.1080/19315260.2011.623761
Kutty C N and Sirohi P S. 2003. Combining ability studies in radish (Raphanus sativus L.). Vegetable Science 30(2): 120–3.
Li J, Ou-Lee T M, Raba R, Amundson R G and Last R L. 1993. Arabidopsis flavonoid mutants are hypersensitive to UV-B irradiation. Plant Cell 5: 171–9. DOI: https://doi.org/10.1105/tpc.5.2.171
Liang Z, Yang C, Yang J, Wu B, Wang L, Cheng J and Li S. 2009. Inheritance of anthocyanins in berries of Vitis vinifera grapes. Euphytica 167: 113–25. DOI: https://doi.org/10.1007/s10681-008-9868-1
Mahan A L, Murray S C, Rooney L W and Crosby K M. 2013. Combining ability for total phenols and secondary traits in a diverse set of coloured (red, blue and purple) maize. Crop Science 53: 1–8. DOI: https://doi.org/10.2135/cropsci2012.06.0385
Matsufuji H, Kido H, Misawa H, Yaguchi J, Otsuki T, Chino M, Takeda M and Yamagata K. 2007. Stability to light, heat, and hydrogen peroxide at different pH values and DPPH radical scavenging activity of acylated anthocyanins from red radish extract. Journal of Agricultural and Food Chemistry 55: 3692–3701. DOI: https://doi.org/10.1021/jf063598o
McGhie T K and Walton M C. 2007. The bioavailability and absorption of anthocyanins: towards a better understanding. Molecular Nutrition and Food Research 51: 702–13. DOI: https://doi.org/10.1002/mnfr.200700092
Misyura M. 2014. ‘High density stress response in plants and the role of anthocyanin biosynthesis under adverse environmental conditions.’ Ph D Thesis, Molecular and Cellular Biology, University of Guelph, Guelph, Ontario, Canada.
Mo Y, Nagel C and Taylor L P. 1992. Biochemical complementation of chalcone synthase mutants defines a role for flavonols in functional pollen. Proceedings of the National Academy of Sciences USA 89: 7213–7. DOI: https://doi.org/10.1073/pnas.89.15.7213
Panse V G and Sukhatme P V. 1967. Statistical Methods for Agricultural Workers. Indian Council of Agricultural Research (ICAR), New Delhi, India.
Peng C H, Liu L K, Chuang C M, Chyau C C, Huang C N and Wang C J. 2011. Mulberry water extracts possess an anti-obesity effect and ability to inhibit hepatic lipogenesis and promote lipolysis. Journal of Agricultural and Food Chemistry 59: 2663–71. DOI: https://doi.org/10.1021/jf1043508
Pojer E, Mattivi F, Johnson D and Stockley C S. 2013. The case for anthocyanin consumption to promote human health: A review. Comprehensive Reviews in Food Science and Food Safety 12: 483–508. DOI: https://doi.org/10.1111/1541-4337.12024
Prior R L and Wu X. 2006. Anthocyanins: structural characteristics that result in unique metabolic patterns and biological activities. Free Radical Research 40: 1014–28. DOI: https://doi.org/10.1080/10715760600758522
Rahman M M, Ichiyanagi T, Komiyama T, Hatano Y and Konishi T. 2006. Superoxide radical- and peroxynitrite-scavenging activity of anthocyanins; structure-activity relationship and their synergism. Free Radical Research 40: 993–1002. DOI: https://doi.org/10.1080/10715760600815322
Rodriguez V M, Soengas P, Landa A, Ordas A and Revilla P. 2013. Effects of selection for color intensity on antioxidant capacity in maize (Zea mays L.). Euphytica 193: 339–45. DOI: https://doi.org/10.1007/s10681-013-0924-0
Ross J A and Kasum C M. 2002. Dietary flavonoids: bioavailability, metabolic effects and safety. Annual Review of Nutrition 22: 19–34. DOI: https://doi.org/10.1146/annurev.nutr.22.111401.144957
Searle S R. 1961. Phenotypic, genotypic and environmental correlations. Biometrics 17: 474–80. DOI: https://doi.org/10.2307/2527838
Shirley B W. 1996. Flavonoid biosynthesis: ‘‘new’’ functions for an ‘‘old’’ pathway. Trends in Plant Science 1: 377–82. DOI: https://doi.org/10.1016/1360-1385(96)10040-6
Singh A, Singh B K, Deka B C, Sanwal S K, Patel R K and Verma M R. 2011. The genetic variability, inheritance and inter-relationships of ascorbic acid, β-carotene, phenol and anthocyanin content in strawberry. Scientia Horticulturae 129(1): 86–90. DOI: https://doi.org/10.1016/j.scienta.2011.03.011
Singh B K. 2007. ‘Studies on vand heterosis of important economic and nutritive traits in cabbage.’ Ph D Thesis. IARI, Pusa, New Delhi, India.
Singh B K and Karmakar P. 2015. Improved production technology for root crops. (In): Improved Production Technologies in Vegetable Crops, pp. 120–33. Singh N, Roy S, Karmakar P, Chaurasia S N S, Gupta S and Singh B (Eds). IIVR Training Manual No. 59, Indian Institute of Vegetable Research, Varanasi.
Singh B K, Sharma S R and Singh B. 2010. Antioxidant enzymes in cabbage: variability and inheritance of superoxide dismutase, peroxidase and catalase. Scientia Horticulturae 124(1): 9–13. DOI: https://doi.org/10.1016/j.scienta.2009.12.011
Singh R K and Chaudhary B D. 1977. Biometrical Methods in Quantitative Genetic Analysis. Kalyani Publishers, Ludhiana, India.
Singleton V L, Orthofer R and Lamuela-Ranventos R M. 1999. Analysis of total phenols other oxidation substrates and antioxidants by means of Folin–Ciocalteu reagent. Methods in Enzymology 299: 152–78. DOI: https://doi.org/10.1016/S0076-6879(99)99017-1
Soengas P, Sotelo T, Velasco P and Cartea M E. 2011. Antioxidant properties of Brassica vegetables. Functional Plant Science and Biotechnology 5(2): 43–55.
Tatsuzawa F, Saito N, Toki K, Shinoda K, Shigihara A and Honda T. 2010. Acylated cyanidin 3-sophoroside-5-glucosides from the purple roots of red radish (Raphanus sativus L.) ‘Benikanmi’. Journal of the Japanese Society for Horticultural Science 79(1): 103–7. DOI: https://doi.org/10.2503/jjshs1.79.103
Tsuda T. 2012. Dietary anthocyanin-rich plants: biochemical basis and recent progress in health benefits studies. Molecular Nutrition and Food Research 56: 159–70. DOI: https://doi.org/10.1002/mnfr.201100526
Wang L S and Stoner G D. 2008. Anthocyanins and their role in cancer prevention. Cancer Letter 269: 281–90. DOI: https://doi.org/10.1016/j.canlet.2008.05.020
Wrolstad R E, Durst R W and Lee J. 2005. Tracking color and pigment changes in anthocyanin products. Trends in Food Science and Technology 16(9): 423–8. DOI: https://doi.org/10.1016/j.tifs.2005.03.019
Zilic S, Serpen A, Akıllioglu G, Gokmen V and Vancetovic J. 2012. Phenolic compounds, carotenoids, anthocyanins and antioxidant capacity of colored maize (Zea mays L.) kernels. Journal of Agricultural and Food Chemistry 5: 1224–31. DOI: https://doi.org/10.1021/jf204367z
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