Ferreting out blast disease resistance in advanced breeding lines of rice (Oryza sativa) by phenotypic evaluation and microsatellite markers

J P BHATT

doi:10.56093/ijas.v88i3.78401

Authors

J P BHATT Senior Research Assistant, Regional Cotton Research Station, Anand Agricultural University, Viramgam, Ahmedabad, Gujarat 387 540

https://doi.org/10.56093/ijas.v88i3.78401

Keywords:

Blast disease, Disease resistance, Microsatellite markers, Resistance genes, Rice

Abstract

Rice (Oryza sativa L.) is the second most important cereal crop of developing countries and the staple food of about 65% of the world's population. Rice blast is a serious disease affecting rice, caused by a fungal pathogen Magnaporthe grisea (anamorph - Pyricularia grisea). In this endeavor, it is important to identify durable resistance sources. Forty two elite advanced breeding lines containing local and IRRI cultivars in their parentage were screened for the blast disease in uniform blast nursery during wet season of 2012 and 2013 using local land race Pankhali-203 as an inoculum spreader. Four elite lines, viz. GR-7/CRMAS-2231-36(8-4-1-1-1), GR-7/DDR-8(1-5-1-1), IR-28/IET-16804(4-1-1-1-1,2) and Jaya/IR-64(52-2-3-2) and GR-7, a released cultivar, were found highly resistant. Screening of these lines was also done using gene specific eighteen SSR markers. All markers amplified a total of 757 bands with an average of 42.10 bands per marker. The SSR marker RM535 for Pib gene and RM28050 for Pi20 gene produced maximum number (19) of alleles. Most of the breeding lines were found to have all the resistance genes with a diversity of allele range. The diversity analysis revealed the clustering pattern of these genotypes based on their parentage and not the genes present. The highest similarity index value of 0.53 was found between GR-7/NWGR-2002(3-1-1-1-1) and GR-7/NWGR-2002(2-3-1-2-1). Thus, present study revealed the resistance source for rice blast disease from advanced breeding lines simultaneously with the resistance genes present in them.

Downloads

Download data is not yet available.

References

Ashkani S, Rafii M Y, Rusli I, Sariah M, Abdullah S N A, Rahim H A and Latif M A. 2012. SSRs for marker-assisted selection for blast resistance in rice (Oryza sativa L.). Plant Molecular Biology Report. 30: 79–86. DOI: https://doi.org/10.1007/s11105-011-0315-4

Bonman J M, Estrada B A and Bandong J M. 1989. Leaf and neck blast resistance in tropical lowland rice cultivars. Plant Disease 73: 388–90. DOI: https://doi.org/10.1094/PD-73-0388

Bonman J M. 1992. Durable resistance to rice blast disease-environmental influences. Euphytica 63(1-2): 115–123. DOI: https://doi.org/10.1007/BF00023917

Chen X, Temnykh S, Xu Y, Cho Y G and McCouch S R. 1997. Development of a microsatellite framework map providing genome-wide coverage in rice (Oryza sativa L.). Theoretical and Applied Genetics. 95: 553. DOI: https://doi.org/10.1007/s001220050596

Dean R A, Lee Y H, Mitchell T K, and Whitehead D S. 1994. Signalling system and gene expression regulating appressorium formation in Magnaporthe grisea. Rice Blast Disease. 2: 23–6.

Eizenga G C, Agama H A, Lee F N and Jia Y. 2006. Identifying novel resistance genes in newly introduced blast resistant rice germplasm. Crop Science 46: 1870–1878. DOI: https://doi.org/10.2135/cropsci2006.0143

Ghaley B B, Christiansen J L and Andersen S B. 2012. Genetic diversity in blast resistance of Bhutan rice landraces. Euphytica, 184: 119–30. DOI: https://doi.org/10.1007/s10681-011-0582-z

Idowu O O, Salami A O, Ajayi S A, Akinwale R O and Sere Y. 2013. Varietal resistance of rice to blast fungus Magnaporthe oryzae at two sites in southwestern Nigeria. African Journal of Biotechnology 12(33): 5173–82. DOI: https://doi.org/10.5897/AJB2012.2959

IRRI. 1996. Standard Evaluation System for Rice. International Rice Testing Programme. IRRI. Philippines.

Jaccard P. 1908. Nouvelles researches sur la distribution florale. Bull. Soc. Vaudoise Sci. Natl. 44: 223–70.

Jia Y L, Bryan G T and Farrall L 2004. Natural variation at the Pi-ta rice blast resistance locus. Phytopathology 93: 1452–9. DOI: https://doi.org/10.1094/PHYTO.2003.93.11.1452

Jia Y, Wang Z and Singh P. 2002. Development of dominant rice blast Pi-ta resistance gene markers. Crop science 42: 2145–9. DOI: https://doi.org/10.2135/cropsci2002.2145

Kim H K, Lee S K, Cho J I, Lee S, An G, Jwa N S, Kim B R, Cho Y C, Han S S, Bhoo S H, Lee Y H, Hong Y K, Yi G, Park D S, Hahn T R and Jeon J S. 2005. Characterization of rice mutants with enhanced susceptibility to rice blast. Molecular Cells 20(3): 385–91.

Li W, Lei C, Cheng Z, Jia Y, Huang D, Wang J, Wang J, Zhang X, Su N and Guo X. 2008. Identification of SSR markers for a broad-spectrum blast resistance gene Pi20(t) for marker-assisted breeding. Molecular Breeding 22: 141–9. DOI: https://doi.org/10.1007/s11032-008-9163-9

Liu G, Bernhardt J L, Jia M H, Wamishe Y A and Jia Y. 2008. Molecular characterization of the recombinant inbred line population derived from a japonica-indica rice cross. Euphytica 159 (1-2): 73–82. DOI: https://doi.org/10.1007/s10681-007-9459-6

McRae W. 1922. Report of the imperial mycologist. Scientific Report, Pusa Agricultural Research Institute, 1921 – 22, pp 44 – 50.

Padmanabhan S Y. 1965. Breeding for blast resistance in India. (In) Rice Blast Disease, pp. 203 –21. Johns Hopkins Press, Baltimore, Maryland, USA.

Rathour R, Chopra M and Sharma T R. 2008. Development and validation of microsatellite markers linked to the rice blast resistance gene Pi-z of Fukunishiki and Zenith. Euphytica 163: 275–82. DOI: https://doi.org/10.1007/s10681-008-9646-0

Ravikumar B N V S R, Koteswara Rao K, Chamundeswari N, Bhuvaneswari V, Nagakumari, P, Venkata Ramana Rao P, Girija Rani M, Satyanarayana P V, Suryanarayana Y, Bharatalakshmi M and Vishnuvardhan Reddy A. 2014. Screening of advanced breeding lines for seedling blast resistance in rice (Oryza sativa L.) using microsatellite markers Current Biotica 8 (2): 125–31.

Rohlf F J. 1994. NTSYS-PC Numerical Taxonomy and Multivariate analysis System, ver. 2.02. State University of New York, Stonybrook, New York.

Roumen E C. 1992. Small differential interactions for partial resistance in rice cultivars to virulent isolates of the blast pathogen. Euphytica 64: 143–8. DOI: https://doi.org/10.1007/BF00023548

Shrestha S M and Mishra N K. 1994. Evaluation of common cultivars of rice against leaf and neck blast in Nepal. Nepal Journal of Agriculture and Animal Science 15: 101–3.

Wang G L, Mackill D J, Bonman J M, McCouch S R, Champox M C and Nelson R J. 1994. RFLP mapping of genes conferring complete and partial resistance to blast in a durably resistant rice cultivar. Genetics 136: 1421–34. DOI: https://doi.org/10.1093/genetics/136.4.1421

Yu Z H, Mackill D J and Bonman J M. 1987. Inheritance of resistance to blast in some traditional and improved rice cultivars. Phytopathology 77(2): 323–6. DOI: https://doi.org/10.1094/Phyto-77-323

Zidani S, Ferchichi A and Chaieb M. 2005. Genomic DNA extraction method from pearl millet (Pennisetum glaucum) leaves. African Journal of Biotechnology 4(8): 862–6.