Marker assisted screening of F2 population for late blight (Phytophthora infestans) resistance in indeterminate tomato (Solanum lycopersicum) under protected condition

MANISHA DUBEY; M S DHALIWAL; S K JINDAL; ABHISHEK SHARMA

doi:10.56093/ijas.v88i4.79110

Authors

MANISHA DUBEY Women Scientist, Punjab Agricultural University, Ludhiana, Punjab 141004
M S DHALIWAL Additional Director of Research (Horticulture and Food Science), Punjab Agricultural University, Ludhiana, Punjab 141004
S K JINDAL Assistant Vegetable Breeder, Punjab Agricultural University, Ludhiana, Punjab 141004
ABHISHEK SHARMA Assistant Virologist, Department of Vegetable Science, Punjab Agricultural University, Ludhiana, Punjab 141004

https://doi.org/10.56093/ijas.v88i4.79110

Keywords:

Markers assisted selection, Phytophthora infestans, Resistance breeding, Solanum lycopersicum

Abstract

Late blight (LB) (Phytophthora infestans (Mont.) de Bary) is a highly destructive disease of tomato (Solanum lycopersicum L.) worldwide and attacks all the aerial parts of the plant including leaves, stems and immature green fruits causing 100% yield loss under congenial environmental conditions. The objective of this study was to identify molecular markers associated with Ph-2 and Ph-3 gene conferring LB resistance in tomato. Two co-dominant markers (TG422 and TG328) were associated with Ph-2 and Ph-3, which were cleaved amplified polymorphic sequences (CAPS) type. The tomato line PAU IND 2372 was crossed with the late blight resistant line LBR 21 to develop F2 population. A total of 540 plants were screened for the target loci and tagged. From 540 F2 plants, 112 were resistant for Ph-2 and 106 for Ph-3 gene and 281 plants were positive for both Ph-2 and Ph-3 gene. The pattern of segregation of susceptibility or resistance was investigated using the chi-square (χ2) test.

Downloads

Download data is not yet available.

References

Becktell M C, Daughtrey M L and Fry W E. 2005. Epidemiology and management of petunia and tomato late blight in the greenhouse. Plant Disease 89: 1000–8. DOI: https://doi.org/10.1094/PD-89-1000

Brouwer D J and St. Clair D A. 2004. Fine maping of three quantitative trait loci for late blight resistance in tomato using near isogenic lines (NILS) and sub – NILS. Theoretical and Applied Genetics 108: 628–38. DOI: https://doi.org/10.1007/s00122-003-1469-8

Chowdappa P, Nirmal Kumar B J, Madhura S, Kumar Mohan S P, Myers K L, Fry W E, Squires J N and Cooke D E L. 2013. Emergence of 13_A2 Blue lineage of Phytophthora infestans was responsible for severe outbreaks of late blight on tomato in south-west India. Journal of Phytopathology 161: 49–58. DOI: https://doi.org/10.1111/jph.12031

Chowdappa P, Nirmal Kumar B J, Madhura S, Kumar Mohan S P, Myers K L, Fry W E and Cooke D E L. 2014. Severe outbreaks of late blight on potato and tomato in South India caused by recent changes in the Phytophthora infestans population. Plant Pathology 64: 191–9. DOI: https://doi.org/10.1111/ppa.12228

Dorrance A E and Inglis D A.1997. Assessment of greenhouse and laboratory screening methods for evaluating potato foliage for resistance to late blight. Plant Disease 81: 1206–13. DOI: https://doi.org/10.1094/PDIS.1997.81.10.1206

FAOSTAT. 2013. FAO Statistical Databases. Food and Agriculture Organization of the United Nations, Statistics Division (http:// faostat.fao.org).

Foolad M R, Merk H L and Ashrafi H. 2008: Genetics, genomics and breeding of late blight and early blight resistance in tomato. Critical Review on Plant Science 27: 75–107. DOI: https://doi.org/10.1080/07352680802147353

Fry W. 2008. Phytophthora infestans: the plant (and R gene) destroyer. Molecular Plant Pathology 9: 385–402. DOI: https://doi.org/10.1111/j.1364-3703.2007.00465.x

Foolad M R and Panthee D R. 2012. Marker assisted selection in tomato breeding. Critical Review on Plant Sciences 31: 93–123. DOI: https://doi.org/10.1080/07352689.2011.616057

Fulton T M, Chunwongse J and Tanksley S D. 1995. Microprep protocol for extraction of DNA from tomato and other herbaceous plants. Plant Molecular Biology Reporter 13: 207–9. DOI: https://doi.org/10.1007/BF02670897

Gotoh K, Akino S, Maeda A, Kondo N, Naito S, Kato M and Ogoshi A. 2005. Characterization of some Asian isolates of Phytophthora infestans. Plant Pathology 54: 733–9. DOI: https://doi.org/10.1111/j.1365-3059.2005.01286.x

Hakiza J J. 1999. The importance of resistance to late blight in potato breeding in Africa. (In) Proceedings of the Global Initiative on Late Blight Conference, Quito, Ecuador, March 16–19, p 4.

Li J, Liu L, Bai Y, Finkers R and Wang F. 2011. Identification and mapping of quantitative resistance to late blight (Phytophthora infestans) in Solanum habrochaites LA1777. Euphytica 179: 427–38. DOI: https://doi.org/10.1007/s10681-010-0340-7

Merk H L, Ashrafi H and Foolad M R. 2012. Selective genotyping to identify late blight resistance genes in an accession of the tomato wild species Solanum pimpinellifolium. Euphytica 187: 63–75. DOI: https://doi.org/10.1007/s10681-012-0729-6

Michalska A M, Zimnoch-Guzowska E, Sobkowiak S and Plich J. 2011. Resistance of potato to stem infection by Phytophthora infestans and a comparison to detached leaflet and field resistance assessments. American Journal of Potato Research 88: 367–73. DOI: https://doi.org/10.1007/s12230-011-9202-7

Mukalazi J, Adipala E, Sengooba T, Hakiza J J, Olanya M and Kidanemariam H M. 2001. Variability in potato late severity and its effect on tuber yield in Uganda. African Crop Science Journal 9: 195–201. DOI: https://doi.org/10.4314/acsj.v9i1.27639

Nilson H E. 2006. Bioassay to detect small differences in resistance of tomato to late blight according to leaf age, leaf and leaflet position, and plant age. Australasian Plant Pathology 35: 297–301. DOI: https://doi.org/10.1071/AP06020

Nowicki M, Foolad M R, Nowakowska M and Kozik E U. 2012. Potato and tomato late blight caused by Phytophthora infestans: An overview of pathology and resistance breeding. Plant Disease 96: 4–17. DOI: https://doi.org/10.1094/PDIS-05-11-0458

Pearson K. 1922. On the chi – square test of goodness of fit. Biometrika 14: 186–418. DOI: https://doi.org/10.2307/2331860

Robbins M D, Masud M A T, Panthee D R, Gardner C O, Francis D and Stevens M A.2010. Marker –assisted selection for coupling phase resistance to tomato spotted wilt virus and Phytophthora infestans in tomato. Hort Science 45: 1424–8. DOI: https://doi.org/10.21273/HORTSCI.45.10.1424

Thind T S, Moha C, Sokhi S S and Bedi J S. 1989. A detached leaf technique for maintenance and multiplication of Phytophthora infestans and evaluation of fungicides. Current Science 58: 388–9.

Wilson C and Tisdell C. 2001. Why farmers continue to use pesticides despite environmental health and sustainability costs. Ecological Economics 39: 449–62. DOI: https://doi.org/10.1016/S0921-8009(01)00238-5