Application of loop-mediated isothermal amplification (LAMP) in the detection of genetically modified RT 73 rapeseed: a qualitative fast inspection

JINHUA LIU; YANYU SHI; KUN LIU; HAOTIAN WANG

doi:10.56093/ijas.v88i4.79130

Authors

JINHUA LIU School of Pharmaceutical Sciences, Jilin University, 1163 Xinmin Street, Changchun, Jilin 130000, China
YANYU SHI Jilin Province Food Inspection Institute, 2699 Yiju Road, Changchun, Jilin 130000, China
KUN LIU School of Pharmaceutical Sciences, Jilin University, 1163 Xinmin Street, Changchun 130000, Jilin Province, China
HAOTIAN WANG School of Pharmaceutical Sciences, Jilin University, 1163 Xinmin Street, Changchun 130000, Jilin Province, China

https://doi.org/10.56093/ijas.v88i4.79130

Keywords:

Genetically modified rapeseed, Isothermal condition, Loop-mediated isothermal amplification, Rapid detection

Abstract

The study intended to establish a rapid, simple, efficient and sensitive method for detecting transgenic RT 73 rapeseed using the loop-mediated isothermal amplification (LAMP) technology. The transgenic RT 73 rapeseed sequences locating at the interface of endogenous gene to exogenous gene was taken as the target sequences, and four LAMP primers, inner primers FIP, BIP, and outer primers F3, B3 were designed, with specific identification to six independent regions of target genes. The LAMP reaction was performed. The LAMP primers specifically amplified the exogenous DNA of transgenic RT 73 rapeseed and the detection limit was 0.01ng DNA.The results could be determined with the help of real-time turbidimeter or directly observed by eyes for the qualitative determination. LAMP was specific to amplify exogenous genes of genetically modified RT 73 rapeseed, and the detection limit of 0.1 ng DNA was similar to that of real-time PCR. The LAMP operation was simple to greatly improve the efficiency of transgenic detection and have a good application prospect.

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