Quantification of total endogenous ascorbic acid from chrysanthemum (Chrysanthemum × morifolium) cultivars and its association with postharvest life

VARUN M HIREMATH; RITU JAIN; NEELU JAIN; AJAY ARORA; KISHAN SWAROOP; M K SINGH; PRABHAT KUMAR; GUNJEET KUMAR

doi:10.56093/ijas.v88i9.83506

Authors

VARUN M HIREMATH ICAR-Indian Agricultural Research Institute, New Delhi 110 012
RITU JAIN ICAR-Indian Agricultural Research Institute, New Delhi 110 012
NEELU JAIN Division of Genetics, ICAR-Indian Agricultural Research Institute, New Delhi 110 012
AJAY ARORA Division of Plant Physiology, ICAR-Indian Agricultural Research Institute, New Delhi 110 012
KISHAN SWAROOP ICAR-Indian Agricultural Research Institute, New Delhi 110 012
M K SINGH ICAR-Indian Agricultural Research Institute, New Delhi 110 012
PRABHAT KUMAR ICAR-Indian Agricultural Research Institute, New Delhi 110 012
GUNJEET KUMAR Division of Floriculture and Landscaping, ICAR-Indian Agricultural Research Institute, New Delhi 110 012

https://doi.org/10.56093/ijas.v88i9.83506

Keywords:

Antioxidants, Ascorbic acid, Leaf longevity, Senescence, Vase life

Abstract

In the present study, an effort was made to quantify the total endogenous ascorbic acid to reveal if it has any association with vase life of chrysanthemum (Chrysanthemum × morifolium Ramat) cultivars. Among 30 cultivars, maximum total ascorbic acid content (441.29 mg/100 g fresh weight) was recorded in cv. Red Gold followed by cv. Jaya (336.34 mg/100 g fresh weight) and minimum ascorbic acid content (93.55 mg/100 g fresh weight) was observed in cv. Arka Ravi followed by cv. Yellow Reflex (101.76 mg/100 g fresh weight). Chrysanthemum cultivars were found to contain maximum total ascorbic acid content in petals (235.00 mg/100 g fresh weight) and leaves (285.12 mg/100 g fresh weight) in stage II (fully opened flower). During all the stages of flower development total ascorbic acid content in leaves was recorded higher as compared to petals. It is concluded that there is a significant association between postharvest life and ascorbic acid content in leaves (r = 0.669) and petals (r = 0.547) at fully opened flower stage in chrysanthemum.

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References

Attolico A D and De Tullio M C. 2006. Increased ascorbate content delays flowering in long-day grown Arabidopsis thaliana (L.) Heynh. Plant Physiology and Biochemistry 44(7): 462–6. DOI: https://doi.org/10.1016/j.plaphy.2006.08.002

Barth C, De Tullio M and Conklin P L. 2006. The role of ascorbic acid in the control of flowering time and the onset of senescence. Journal of Experimental Botany 57(8): 1657–65. DOI: https://doi.org/10.1093/jxb/erj198

Bartoli C G, Simontacchi M, Montaldi E R and Puntarulo S. 1997. Oxidants and antioxidants during aging of Chrysanthemum petals. Plant Science 129: 157–65. DOI: https://doi.org/10.1016/S0168-9452(97)00197-0

Cavaiuolo M, Cocetta G and Ferrante A. 2013. The antioxidants changes in ornamental flowers during development and senescence. Antioxidants 2(3): 132–55. DOI: https://doi.org/10.3390/antiox2030132

Cheruth A J, Kurup S S and Subramaniam S. 2015. Variations in hormones and antioxidant status in relation to flowering in early, mid and late varieties of date palm (Phoenix dactylifera) of United Arab Emirates. Scientific World Journal 2015: 1–8 DOI: https://doi.org/10.1155/2015/846104

Conklin P L and Barth C. 2004. Ascorbic acid, a familiar small molecule intertwined in the response of plants to ozone, pathogens and the onset of senescence. Plant, Cell & Environment 27(8): 959–70. DOI: https://doi.org/10.1111/j.1365-3040.2004.01203.x

Doi M, Nakagawa Y, Watabe S, Aoe K, Inamoto K and Imanishi H. 2003. Ethylene-induced leaf yellowing in cut chrysanthemums (Dendranthema grandiflora Kitamura). Journal of the Japanese Society for Horticultural Science 72(6): 533–5. DOI: https://doi.org/10.2503/jjshs.72.533

Ferrante A, Mensuali-Sodi A, Tognoni F and Serra G. 2005. Postharvest studies on leaf yellowing of chrysanthemum cut flowers. Advances in Horticultural Science 19(2): 81–5.

Foyer C H, Lelandais M, Edwards E A and Mullineaux P M. 1991. The role of ascorbate in plants, interactions with photosynthesis and regulatory significance. In: E Pell and K Steffen eds. Active Oxygen/Oxidative Stress and Plant Metabolism: Current Topics in Plant Physiology, American Society of Plant Physiologists, Rockville, M D 6: 131–44.

Franceschi V R and Tarlyn N M. 2002. L-Ascorbic acid is accumulated in source leaf phloem and transported to sink tissues in plants. Plant Physiology 130(2): 649–56. DOI: https://doi.org/10.1104/pp.007062

Gan S and Amasino R M. 1997. Making sense of senescence (molecular genetic regulation and manipulation of leaf senescence). Plant Physiology 113(2): 313–9. DOI: https://doi.org/10.1104/pp.113.2.313

Halevy A H and Mayak S. 1979. Senescence and postharvest physiology of cut flowers—Part 1. Horticultural Reviews 3: 59–143. DOI: https://doi.org/10.1002/9781118060766.ch3

Harman D. 1956. Aging: a theory based on free radical and radiation chemistry. Journal of Gerontology 11(3): 298–300. DOI: https://doi.org/10.1093/geronj/11.3.298

Hensel L L, Grbić V, Baumgarten D A and Bleecker A B. 1993. Developmental and age-related processes that influence the longevity and senescence of photosynthetic tissues in Arabidopsis. Plant Cell 5(5): 553–64. DOI: https://doi.org/10.1105/tpc.5.5.553

Jain R, Janakiram T, Singh K P and Kumawat G L. 2014. Reduction of foliage discoloration and extending vase life of chrysanthemum (Dendranthema × grandiflora) with different floral preservatives. Indian Journal of Agricultural Sciences 84(10): 1250–3.

Kofranek A M and Halevy A H. 1980. Chemical pretreatment of chrysanthemum before shipment. In II International Symposium on Post-harvest Physiology of Cut Flowers 113: 89–96. DOI: https://doi.org/10.17660/ActaHortic.1981.113.13

Kotchoni S O, Larrimore K E, Mukherjee M, Kempinski C F and Barth C.2009. Alterations in the endogenous ascorbic acid content affect flowering time in Arabidopsis. Plant Physiology 149: 803–15. DOI: https://doi.org/10.1104/pp.108.132324

Law M Y, Charles S A and Halliwell B. 1983. Glutathione and ascorbic acid in spinach (Spinacia oleracea) chloroplasts. The effect of hydrogen peroxide and paraquat. Biochemical Journal 210(3): 899–903. DOI: https://doi.org/10.1042/bj2100899

Loewus F A, Loewus M W and Seib P A. 1987. Biosynthesis and metabolism of ascorbic acid in plants. Critical Reviews in Plant Sciences 5(1): 101–19. DOI: https://doi.org/10.1080/07352688709382235

Mukherjee S P and Choudhari M A. 1983. Implications of water stress-induced changes in the levels of endogenous ascorbic acid and hydrogen peroxide in vigna seedlings. Physiologia Plantarum 58(2): 166–70. DOI: https://doi.org/10.1111/j.1399-3054.1983.tb04162.x

Pastori G M, Kiddle G, Antoniw J, Bernard S, Veljovic-Jovanovic S, Verrier P J and Foyer C H. 2003. Leaf vitamin C contents modulate plant defense transcripts and regulate genes that control development through hormone signaling. Plant Cell 15(4): 939–51. DOI: https://doi.org/10.1105/tpc.010538

Pavet V, Olmos E, Kiddle G, Mowla S, Kumar S, Antoniw J and Foyer C H. 2005. Ascorbic acid deficiency activates cell death and disease resistance responses in Arabidopsis. Plant Physiology 139(3): 1291–1303. DOI: https://doi.org/10.1104/pp.105.067686

Petridou M, Voyiatzi C and Voyiatzis D. 2001. Methanol, ethanol and other compounds retard leaf senescence and improve the vase life and quality of cut chrysanthemum flowers. Postharvest Biology and Technology 23: 79–83. DOI: https://doi.org/10.1016/S0925-5214(01)00102-8

Smart C M. 1994. Gene expression during leaf senescence. New Phytologist 126(3): 419–48. DOI: https://doi.org/10.1111/j.1469-8137.1994.tb04243.x

Smirnoff N and Pallanca J E. 1996. Ascorbate metabolism in relation to oxidative stress. Biochemical Society Transactions 24: 472–78. DOI: https://doi.org/10.1042/bst0240472

Sohal R S and Weindruch R. 1996. Oxidative stress, caloric restriction and aging. Science 273(5271): 59. DOI: https://doi.org/10.1126/science.273.5271.59

Zimmermann Petra and Zentgraf Ulrike. 2005. The correlation between oxidative stress and leaf senescence during plant development. Cellular and Molecular Biology Letters 10(3): 515.