Studies on floral sources, phenolic, flavonoid, proline contents and antioxidant capacity of honey from the Khirsoo area of Pauri, Uttarakhand, India

Authors

  • P L UNIYAL Professor, Department of Botany, Delhi University, Delhi 110 007
  • P TIWARI Assistant Professor, HNB Garhwal University, Srinagar, Garhwal, Uttarakhand 246 174
  • EKTA CHAUDHARY Reseach Scholar, Department of Botany and Microbiology, HNB Garhwal University, Srinagar, Garhwal, Uttarakhand 246 174

DOI:

https://doi.org/10.56093/ijas.v88i10.84216

Keywords:

Antioxidant, Flavonoid, Phenolic, Pollen spectra, Proline

Abstract

Present study was carried out to determine the floral sources, phenolic, flavonoid, proline contents and antioxidant capacity of 24 honey samples collected from the Khirsoo area of the Pauri district, Uttarakhand (India). The phenolic, flavonoid, proline contents, and antioxidant capacity of the honey samples ranged from 36.83 to 106.66 mg GAE/100g, 3.99 to 20.56 mg QE/100g, 27.3 to 621.22 mg/kg and 16.24 to 25.96 mg AAE/100g, respectively. Maximum quantity of phenolic, flavonoid contents and antioxidant capacity was found in Thapla honey representing the dominant floral sources Aesculus indica, Rhododendron arboreum, Myrica esculentum, Berberis aristata, Coriandrum sativa, Brassicaceous and Rosaceous members. Highest proline content was found in Gwaad honey, extracted in the month of November with dominant bee forage species Prunus cerasoides, Sesamum indicum, Impatiens balsaminea, Zeamays and Luffa spp. Study has shown that there is a positive correlation between the antioxidant capacity and phenolic contents (R=0.844) as well as in between antioxidants and the flavonoid contents (R=0.694). The observation revealed that the region has a good potential of bee forage species and quality of honey is good for dietary and medicinal purpose.

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References

Alzahrani H A, Alsabehi R, Boukraa L, Abdellah F, Bellik Y and Bakhotmah B A. 2012. Antibacterial and antioxidant potency of floral honeys from different botanical and geographical origins. Molecules 17: 10540–9. DOI: https://doi.org/10.3390/molecules170910540

Bogdanov S. 1999. Honey quality and international regulatory standards: review by the International Honey Commission. Bee World 80(2): 61–9. DOI: https://doi.org/10.1080/0005772X.1999.11099428

Bursell E, Biling K C, Hagrovem J W, McCabem C T and Slack E. 1974. Metabolism of the blood meal in tsetse flies (a review). Acta Tropica Parasit 31: 297–320.

Boulanouar B, Mounir H, Ahmed B and Abdelaziz G. 2017. Total phenolic, flavonoid contents and antioxidant activities of honey and propolis collected from the region of Laghouat (South of Algeria). International Journal of Pharmacognosy and Chinese Medicine 1(2): 000110. DOI: https://doi.org/10.23880/IPCM-16000110

Codex A C. 2001. Revised Standards for Honey, pp 12-1981. Codex Standard Rome, FAO.

Jose M, Demalsy F, Parent J and Alexander A S. 1989. Microscopic analysis of honey from Manitoba, Canada. Journal of Apicultural Research 28(1): 41–9. DOI: https://doi.org/10.1080/00218839.1989.11100819

Geronimo J D and Fritz R. 2001. Proline in Argentine honeys. Proceedings of the 37th International Apicultural Congress, 28 October-1 November 2001, Durban, South Africa.

Erdtman G. 1952. Pollen Morphology and Plant Taxonomy. Angiosperms. Chronica Botanica Co., Waltham, Massachusettes. DOI: https://doi.org/10.1080/11035895209453507

Erdtman G. 1960. The acetolysis method. A revised description. Svensk Botanisk Tidskrift 54: 561–4.

Ferreres F, Andrade P, Tomás-Barberán F A. 1994. Flavonoids from Portuguese heather honey. Zeitschrift für Lebensmittel untersuchung und -Forschung A 199: 32–7. DOI: https://doi.org/10.1007/BF01192949

Free J B. 1993. Insect Pollination of Crops. Academic Press, London-New York.

Gheldof N, Wang X H and Engeseth N J. 2002. Identification and quantification of antioxidant components of honeys from various floral sources. Journal of Agricultural and Food Chemistry 50: 5870–7. DOI: https://doi.org/10.1021/jf0256135

Nayik G A and Nanda V. 2015. Physico-chemical, enzymatic, mineral and colour characterization of three different varieties of honeys from Kashmir valley of India with a multivariate approach. Polish Journal of Food and Nutrition Sciences 65(2): 101–8. DOI: https://doi.org/10.1515/pjfns-2015-0022

Kao Y T, Lu M J and Chen C. 2011. Preliminary analyses of phenolic compounds and antioxidant activities in tea pollen extracts. Journal of Food Drug Analysis 19: 470–7. DOI: https://doi.org/10.38212/2224-6614.2177

Klatt B K, Holzschuh A and Westphal C. 2014. Bee pollination improves crop quality, shelf life and commercial value. Proceedings of the Royal Society B: Biological Sciences 281: 2013–40. DOI: https://doi.org/10.1098/rspb.2013.2440

Krell R. 1996.Value-added Products from Beekeeping. FAO Agricultural Services Bulletin 124: 87–113.

Louveaux J, Maurizio A and Vorwohl G. 1970. Methods of melissopalynology. Bee World 51(3): 125–38. DOI: https://doi.org/10.1080/0005772X.1970.11097312

Maheshwari H K. 1986. Pollen Flora of North-West Himalaya. Association Of Palynotrotigrapher, Janki Bhawan, Narhi Lucknow.

Martos I, Ferreres F, Yao L D, Arcy B, Caffin N and Tomas- Barberan F A. 2000. Flavonoids in monospecific Eucalyptus honeys from Australia. Journal of Agriculture and Food Chemistry 48(10): 4744–8. DOI: https://doi.org/10.1021/jf000277i

Meda A, Lamien C E, Romito M, Millogo J and Nacoulma O G. 2005. Determination of the total phenolic, flavonoid and proline contents in Burkina Fasan honey, as well as their radical scavenging activity. Food Chemistry 91: 571–7. DOI: https://doi.org/10.1016/j.foodchem.2004.10.006

Michener C D. 1974. The Social Behavior of the Bees. Bilknap Press of Harvard University, Cambridge, UK.

Moniruzzaman M, Sulaiman S A, Azlan S A M and Gan S H. 2013. Two-year variations of phenolics, flavonoids and antioxidant contents in Acacia honey. Molecules, 18: 14694–710. DOI: https://doi.org/10.3390/molecules181214694

Ohe von de W, Persano oddo L, Piana M and Martin P. 2004. Harmonized methods of mellissopalynology. Apidologie 35: 237–40. DOI: https://doi.org/10.1051/apido:2004050

Osanai M and Yonezawa Y. 1986. Changes in amino acid pools in the silkworm Bombyx mori during embryonic life. Alanine accumulation and its conversion to proline during diapause. Insect Biochemistry 16: 373–97. DOI: https://doi.org/10.1016/0020-1790(86)90050-8

Ough C. 1969. Rapid determination of proline in grapes and wines. Journal of Food Science 34: 228–30. DOI: https://doi.org/10.1111/j.1365-2621.1969.tb10327.x

Pashte V V and Kulkarni S R. 2015. Role of pollinators in qualitative fruit crop production: a review. Trends in Biosciences 8(15): 3743–9.

Sahney M, Kumar A and Rahi S. 2016. Pollen analysis of honeys from Varanasi district, Uttar Pradesh, India. Biological Foruman International Journal 8(2): 126–33.

Sant'Ana L D, Sousa J P, Salgueiro F B, Lorenzon M C and Castro R N. 2012. Characterization of monofloral honeys with multivariate analysis of their chemical profile and antioxidant activity. Journal of Food Science 77(1): 135–40. DOI: https://doi.org/10.1111/j.1750-3841.2011.02490.x

Saxena S, Gautam S and Sharma A. 2010. Physical, biochemical and antioxidant properties of some Indian honeys. Food Chemistry 118: 391–7. DOI: https://doi.org/10.1016/j.foodchem.2009.05.001

Silici S, Sagdic O and Ekici L. 2010. Total phenolic content, antiradical, antioxidant and antimicrobial activities of Rhododendron honeys. Food Chemistry 121: 238–43. DOI: https://doi.org/10.1016/j.foodchem.2009.11.078

Tiwari P, Tiwari J K, Singh D and Singh D. 2013. Traditional beekeeping with the Indian honey bee (Apis cerana F.) in District Chamoli, Uttarakhand. India. International Journal of Rural Studies 20: 1–6.

Viuda-Martos M, Ruiz–Navajas Y, Fernandez–Lopez J and Perez– Alvarez J A. 2008. Functional Properties of Honey, Propolis, and Royal Jelly. Journal of Food Science 73(9): 117–24. DOI: https://doi.org/10.1111/j.1750-3841.2008.00966.x

White J W and Doner L W. 1980. Honey Composition and Properties, pp 82-91. Beekeeping in the United States Agriculture Handbook.

Woodhouse R P. 1935. Pollen Grains-Their Structure, Identification and Significance in Science and Medicines, First edition. McGraw Hill Book Company, Inc, New York, London.

Zhishen J, Mengcheng T and Jianming W. 1999. The determination of flavonoid contents in mulberry and their scavenging effects on superoxide radicals. Food Chemistry 64(4): 555–9. DOI: https://doi.org/10.1016/S0308-8146(98)00102-2

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2018-10-24

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How to Cite

UNIYAL, P. L., TIWARI, P., & CHAUDHARY, E. (2018). Studies on floral sources, phenolic, flavonoid, proline contents and antioxidant capacity of honey from the Khirsoo area of Pauri, Uttarakhand, India. The Indian Journal of Agricultural Sciences, 88(10), 1537-1542. https://doi.org/10.56093/ijas.v88i10.84216